scholarly journals Maternal administration of dexamethasone stimulates choline-phosphate cytidylyltransferase in fetal type II cells

1987 ◽  
Vol 241 (1) ◽  
pp. 291-296 ◽  
Author(s):  
M Post
Keyword(s):  
Enzyme Activity ◽  
Specific Activity ◽  
Active Form ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Dexamethasone Treatment ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Choline Phosphate

Administration of dexamethasone to pregnant rats at 19 days gestation increased phosphatidylcholine synthesis (45%) from radioactive choline in type II cells. This enhanced synthesis of phosphatidylcholine was accompanied by an increased conversion of choline phosphate into CDP-choline. Similar results were obtained by incubating organotypic cultures of 19-day-fetal rat lung with cortisol. The increased conversion of choline phosphate into CDP-choline correlated with an enhanced choline-phosphate cytidylyltransferase activity (31% after dexamethasone treatment; 47% after cortisol exposure) in the cell homogenates. A similar increase (26% after dexamethasone treatment; 39% after cortisol exposure) was found in the microsomal-associated enzyme. No differences in cytosolic enzyme activity were observed. The specific activity of the microsomal enzyme was 3-4 times that of the cytosolic enzyme. Most of the enzyme activity was located in the microsomal fraction (58-65%). The treatments had no effect on the total amount of enzyme recovered from the cell homogenates. These results, taken collectively, are interpreted to indicate that the active form of cytidylyltransferase in type II cells is the membrane-bound enzyme and that cytidylyltransferase activation in type II cells from fetal rat lung after maternal glucocorticoid administration occurs by binding of inactive cytosolic enzyme to endoplasmic reticulum.

Isolation of alveolar type II cells from fetal rat lung by differential adherence in monolayer culture

1988 ◽  
Vol 960 (3) ◽  
pp. 441-453 ◽  
Author(s):  
Joseph J. Batenburg ◽  
Caroline J.M. Otto-Verberne ◽  
Ank A.W. Ten Have-Opbroek ◽  
Wies Klazinga
Keyword(s):  
Monolayer Culture ◽  
Alveolar Type ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Alveolar Type Ii Cells ◽  
Fetal Rat ◽  
Fetal Rat Lung

Increased expression of CTP:phosphocholine cytidylyltransferase in maturing type II cells

1994 ◽  
Vol 267 (1) ◽  
pp. L25-L32 ◽  
Author(s):  
M. Hogan ◽  
L. J. Zimmermann ◽  
J. Wang ◽  
M. Kuliszewski ◽  
J. Liu ◽  
...  
Keyword(s):  
Rat Liver ◽  
Type Ii Cells ◽  
Mrna Levels ◽  
Type Ii ◽  
Rat Lung ◽  
Protein Levels ◽  
Ctp:Phosphocholine Cytidylyltransferase ◽  
Fetal Rat ◽  
Fetal Rat Lung ◽  
Phosphatidylcholine Synthesis

We previously reported that phosphatidylcholine synthesis increased in fetal rat lung type II cells with advancing gestation. This increase was accompanied by an increase in CTP:phosphocholine cytidylyltransferase activity, which catalyses a rate regulatory step in de novo phosphatidylcholine synthesis by fetal type II cells. To determine whether this increase in cytidylyltransferase activity is due to an increase in cytidylyltransferase protein levels, the gene and protein expression of cytidylyltransferase was investigated in maturing type II cells. The cytidylyltransferase cDNA was cloned from fetal rat type II cells and showed 99% sequence homology with rat liver cDNA. The cDNA detected two mRNA transcripts (1.8 and 7.5 kb) in fetal rat lung. By reverse-transcriptase polymerase chain reaction (RT-PCR) analysis, cytidyltransferase mRNA content increased three-fold in fetal type II cells with advancing gestation, whereas cytidylyltransferase mRNA levels in fibroblasts remained constant. An antibody against rat liver cytidylyltransferase was used to assess cytidylyltransferase protein. Western blotting revealed that cytidylyltransferase protein content increased threefold in the microsomal fraction of type II cells with advancing gestation. The enzyme protein levels in the cytosolic fraction did not significantly change with development. Enzyme activity studies confirmed these latter observations. We conclude that the increase in surfactant phosphatidylcholine synthesis by type II cells at late fetal gestation is due in part to an increase in the amount of cytidylyltransferase protein.

An Ultrastructural Comparison of Rat Lung Cells Grown in an Organotypic Culture System with Lung Tissue Found in the Whole Animal

1976 ◽  
Vol 34 ◽  
pp. 172-173
Author(s):  
Peter J. Del Vecchio ◽  
Edward P. Dougherty ◽  
William H. J. Douglas
Keyword(s):  
Lung Tissue ◽  
Organotypic Culture ◽  
Fetal Lung ◽  
Lung Cells ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Tissue Organization ◽  
Fetal Rat ◽  
Fetal Rat Lung

This study describes the ultrastructure of an organotypic system prepared from rat fetal lung and compares it to lung in rat fetuses. The preparation of the organotypic system has been described elsewhere (1).The organotypic systems in this study are prepared from 18-19 day fetal rat lung and spend two days in culture making the cells a total of 20-21 days old. The fine structure of the type II cells present in this system will be compared to the type II cells in fetal lung at 20-21 days gestation. Because the tissue organization of the organotypic system is more like the organization of fetal lung at an earlier stage (18-19 days gestation) , the cell to cell relationships of the organotypic system are compared to fetal lung tissue at this stage.The histology of the organotypic system is similar to that of the fetal lung in its glandular stage (Fig. 1). The epithelial cells are all columnar and are surrounded by relatively undifferentiated mesenchyme.

Keratinocyte Growth Factor Enhances Maturation of Fetal Rat Lung Type II Cells

1999 ◽  
Vol 20 (3) ◽  
pp. 423-432 ◽  
Author(s):  
Nadia Chelly ◽  
Oumel-Banine Mouhieddine-Gueddiche ◽  
Anne-Marie Barlier-Mur ◽  
Bernadette Chailley-Heu ◽  
Jacques R. Bourbon
Keyword(s):  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Fetal Rat Lung

scholarly journals The ErbB4 receptor in fetal rat lung fibroblasts and epithelial type II cells

2007 ◽  
Vol 1772 (7) ◽  
pp. 737-747 ◽  
Author(s):  
Washa Liu ◽  
Katja Zscheppang ◽  
Sandy Murray ◽  
Heber C. Nielsen ◽  
Christiane E.L. Dammann
Keyword(s):  
Lung Fibroblasts ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Fetal Rat ◽  
Erbb4 Receptor ◽  
Fetal Rat Lung
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