Mechanism for Biliary Cholesterol Supersaturation: Biliary Lipid Secretion Studies in Obesity with and without Gallstones

1979 ◽  
Vol 56 (3) ◽  
pp. 22P-22P ◽  
Author(s):  
A. Reuben ◽  
P. N. Maton ◽  
Y. Quereshi ◽  
R. H. Dowling
Keyword(s):  
Biliary Lipid ◽  
Biliary Cholesterol ◽  
Lipid Secretion

Bile Lipid Secretion in Obese and Non-Obese Individuals with and without Gallstones

1985 ◽  
Vol 69 (1) ◽  
pp. 71-79 ◽  
Author(s):  
A. Reuben ◽  
P. N. Maton ◽  
G. M. Murphy ◽  
R. H. Dowling
Keyword(s):  
Bile Acid ◽  
Acid Secretion ◽  
Biliary Lipid ◽  
Biliary Cholesterol ◽  
Cholesterol Gallstones ◽  
Lipid Secretion ◽  
Biliary Cholesterol Secretion ◽  
Cholesterol Secretion ◽  
Bile Acid Secretion ◽  
Secretion Rates

1. Biliary lipid secretion rates were measured in non-obese and obese individuals with and without cholesterol gallstones, using a steady-state, amino acid duodenal perfusion method. In addition, biliary lipid secretion rates were measured in five obese gallstone patients receiving high-dose chenodeoxycholic acid therapy (16-22 mg day−1 kg−1). 2. Bile acid secretion rates in the non-obese patients with cholesterol gallstones (563+sem 70 μmol/h, n = 6) were significantly lower than in the non-obese controls (1078 + 210 μmol/h, n = 10, P < 0.05), whereas cholesterol secretion rates were similar in the non-obese individuals with and without gallstones (51+7 and 42+4 μmol/h respectively). 3. In the obese, both with and without gallstones, the major abnormality was hypersecretion of cholesterol (107+7 μmol/h, n = 7, and 81 + 15 μmol/h, n = 7, respectively). Both these values were significantly greater than those in the non-obese controls (P < 0.01-0.02). 4. Biliary cholesterol secretion rates correlated significantly with bile acid secretion rates but, for every mole of bile acid secreted, the obese secreted more cholesterol than the non-obese. 5. Chenodeoxycholic acid treatment lowered biliary cholesterol saturation in obese gallstone patients by reducing biliary cholesterol secretion. 6. These results suggest that there are two major types of defect in biliary lipid secretion in gallstone patients: reduced biliary bile acid secretion in non-obese gallstone patients and excessive biliary cholesterol secretion in the obese.

Cholic acid aids absorption, biliary secretion, and phase transitions of cholesterol in murine cholelithogenesis

1999 ◽  
Vol 276 (3) ◽  
pp. G751-G760 ◽  
Author(s):  
David Q.-H. Wang ◽  
Frank Lammert ◽  
David E. Cohen ◽  
Beverly Paigen ◽  
Martin C. Carey
Keyword(s):  
Cholic Acid ◽  
Bile Flow ◽  
Cholesterol Biosynthesis ◽  
Cholesterol Absorption ◽  
Biliary Lipid ◽  
Biliary Cholesterol ◽  
Intestinal Cholesterol Absorption ◽  
Lipid Secretion ◽  
Lithogenic Diet ◽  
Secretion Rates

Cholic acid is a critical component of the lithogenic diet in mice. To determine its pathogenetic roles, we fed chow or 1% cholesterol with or without 0.5% cholic acid to C57L/J male mice, which because of lith genes have 100% gallstone prevalence rates. After 1 yr on the diets, we measured bile flow, biliary lipid secretion rates, hepatic cholesterol and bile salt synthesis, and intestinal cholesterol absorption. After hepatic conjugation with taurine, cholate replaced most tauro-β-muricholate in bile. Dietary cholic acid plus cholesterol increased bile flow and biliary lipid secretion rates and reduced cholesterol 7α-hydroxylase activity significantly mostly via deoxycholic acid, cholate’s bacterial 7α-dehydroxylation product but did not downregulate cholesterol biosynthesis. Intestinal cholesterol absorption doubled, and biliary cholesterol crystallized as phase boundaries shifted. Feeding mice 1% cholesterol alone produced no lithogenic or homeostatic effects. We conclude that in mice cholic acid promotes biliary cholesterol hypersecretion and cholelithogenesis by enhancing intestinal absorption, hepatic bioavailability, and phase separation of cholesterol in bile.

Effect of Phalloidin on Biliary Lipid Secretion in Rats

1980 ◽  
Vol 58 (6) ◽  
pp. 545-548 ◽  
Author(s):  
Marta Dubin ◽  
S. Erlinger
Keyword(s):  
Bile Acid ◽  
Saturation Index ◽  
Circumstantial Evidence ◽  
Cholesterol Concentration ◽  
Biliary Lipid ◽  
Biliary Cholesterol ◽  
Lipid Secretion ◽  
Cholesterol Saturation ◽  
Cholesterol Saturation Index ◽  
Cholesterol Secretion

1. The effect of phalloidin on bile acid, phospholipid and cholesterol secretion into bile was studied in rats with bile fistulae. 2. Phalloidin, when given for 7 days, induced a significant decrease in biliary cholesterol concentration and cholesterol saturation index. Bile acid and phospholipid concentration in bile remained unchanged. This effect was less marked in animals receiving the drug for 3 days, and not detectable in animals treated for 1 day. 3. These results provide circumstantial evidence for the hypothesis that microfilament dysfunction may lead to alterations in cholesterol secretion into bile.

SAT-406-Elevated biliary cholesterol in spite of hepatic ABCG5/G8 deficiency: Biliary lipid secretion in conditional knock-out mice

2019 ◽  
Vol 70 (1) ◽  
pp. e812-e813
Author(s):  
Friederike Poppenborg ◽  
Caroline Bopp ◽  
Annika Nicklas ◽  
Frank Lammert ◽  
Susanne N Weber
Keyword(s):  
Biliary Lipid ◽  
Biliary Cholesterol ◽  
Lipid Secretion ◽  
Knock Out ◽  
Knock Out Mice

The influence of estrogen on hepatic cholesterol metabolism and biliary lipid secretion in rats fed fish oil

1999 ◽  
Vol 1437 (3) ◽  
pp. 367-377 ◽  
Author(s):  
Elena Bravo ◽  
Alfredo Cantafora ◽  
Carla Cicchini ◽  
Michael Avella ◽  
Kathleen M. Botham
Keyword(s):  
Fish Oil ◽  
Cholesterol Metabolism ◽  
Biliary Lipid ◽  
Hepatic Cholesterol ◽  
Lipid Secretion
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