scholarly journals Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme

2002 ◽  
Vol 269 (2) ◽  
pp. 602-609 ◽  
Author(s):  
Eric Record ◽  
Peter J. Punt ◽  
Mohamed Chamkha ◽  
Marc Labat ◽  
Cees A. M. J. J. van den Hondel ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Recombinant Enzyme ◽  
Laccase Gene ◽  
Pycnoporus Cinnabarinus

Prokaryotic expression and characterization of a keratinolytic protease from Aspergillus niger

Biologia ◽  
2015 ◽  
Vol 70 (2) ◽  
Author(s):  
Xiaoling Chen ◽  
Bo Zhou ◽  
Meng Xu ◽  
Gang Jia ◽  
Zhiqing Huang ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Ethylenediaminetetraacetic Acid ◽  
Signal Sequence ◽  
Sodium Dodecyl ◽  
Recombinant Enzyme ◽  
Protease Gene ◽  
Amino Acid Residues ◽  
Triton X ◽  
Keratinolytic Protease

AbstractIn this study, a keratinolytic protease gene (named as kerD) from Aspergillus niger was cloned. The full-length coding sequence of kerD consists of 1,251 bp and encodes a protein with 416 amino acid residues with a molecular mass of 43,831 Da. A DNA fragment encoding mature kerD without its signal sequence was inserted into the expression vector pET30a(+) and successfully expressed in Escherichia coli. The recombinant protein was purified to approximately 100% purity using Ni-IDA affinity chromatography, and identified by Western blot. The recombinant enzyme had an optimal pH of 8.0 and was stable at pH 7.0-9.0. It exhibited an optimal temperature for activity of 70 °C and was stable at 30-50 °C. It was highly inhibited by 1,10-phenanthroline, ethylenediaminetetraacetic acid and sodium dodecyl sulphate, but activated by phenylmethanesulfonyl fluoride, Mg2+, Fe2+, Mn2+, Cu2+, Zn2+, Ca2+, dithiothreitol, Triton X-100, dimethyl sulfoxide and isopropyl alcohol. The recombinant enzyme could hydrolyse a broad range of protein substrates.

Homologous expression of the feruloyl esterase B gene from Aspergillus niger and characterization of the recombinant enzyme

2004 ◽  
Vol 37 (1) ◽  
pp. 126-133 ◽  
Author(s):  
Anthony Levasseur ◽  
Isabelle Benoit ◽  
Michèle Asther ◽  
Marcel Asther ◽  
Eric Record
Keyword(s):  
Aspergillus Niger ◽  
Recombinant Enzyme ◽  
Feruloyl Esterase ◽  
Homologous Expression

Cloning and characterization of a second laccase gene from the lignin-degrading basidiomycete Pycnoporus cinnabarinus

Gene ◽  
1999 ◽  
Vol 236 (1) ◽  
pp. 169-177 ◽  
Author(s):  
Ulrike Temp ◽  
Uwe Zierold ◽  
Claudia Eggert
Keyword(s):  
Laccase Gene ◽  
Pycnoporus Cinnabarinus

Extraction and Characterization of Antibacterial Compound from Aspergillus niger

2013 ◽  
Vol 16 (4) ◽  
pp. 167-174 ◽  
Author(s):  
Abdulwahid B. A. Al-Shaibani ◽  
◽  
Faiz I. Al-Shakarchi ◽  
Rasha S. Ameen ◽  
◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Antibacterial Compound

scholarly journals Purification and characterization of endoxylanase Xln-1 from Aspergillus niger B03

2009 ◽  
Vol 25 (12) ◽  
pp. 2095-2102 ◽  
Author(s):  
Georgi Dobrev ◽  
Boriana Zhekova ◽  
Ginka Delcheva ◽  
Lidia Koleva ◽  
Nicola Tziporkov ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Purification And Characterization

Characterization of the starvation-induced chitinase CfcA and α-1,3-glucanase AgnB of Aspergillus niger

2014 ◽  
Vol 99 (5) ◽  
pp. 2209-2223 ◽  
Author(s):  
Jolanda M. van Munster ◽  
Justyna M. Dobruchowska ◽  
Ruud Veloo ◽  
Lubbert Dijkhuizen ◽  
Marc J. E. C. van der Maarel
Keyword(s):  
Aspergillus Niger

Geranylgeranylglyceryl Phosphate Synthase. Characterization of the Recombinant Enzyme fromMethanobacterium thermoautotrophicum†

Biochemistry ◽  
2001 ◽  
Vol 40 (49) ◽  
pp. 14847-14854 ◽  
Author(s):  
Tim Soderberg ◽  
Anjun Chen ◽  
C. Dale Poulter
Keyword(s):  
Recombinant Enzyme ◽  
Phosphate Synthase
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