Molecular cloning and characterization of a putative cyclic nucleotide-gated channel from Drosophila melanogaster

2000 ◽  
Vol 9 (3) ◽  
pp. 283-292 ◽  
Author(s):  
M. Miyazu ◽  
T. Tanimura ◽  
M. Sokabe
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Cloning ◽  
Cyclic Nucleotide ◽  
Gated Channel

Characterization of a novel cyclic nucleotide-gated channel from zebrafish brain

2006 ◽  
Vol 348 (2) ◽  
pp. 441-449 ◽  
Author(s):  
Michelle L. Tetreault ◽  
Diane Henry ◽  
Diana M. Horrigan ◽  
Gary Matthews ◽  
Anita L. Zimmerman
Keyword(s):  
Cyclic Nucleotide ◽  
Zebrafish Brain ◽  
Gated Channel

Molecular cloning and characterization of the inositol 1,4,5-triphosphate receptor in Drosophila melanogaster

1992 ◽  
Vol 17 ◽  
pp. 140 ◽  
Author(s):  
Shingo Yoshikawa ◽  
Teiichi Tanimura ◽  
Atsushi Miyawaki ◽  
Makoto Nakamura ◽  
Michisuke Yuzaki ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Molecular Cloning

scholarly journals Characterization of TbPDE2A, a Novel Cyclic Nucleotide-specific Phosphodiesterase from the Protozoan ParasiteTrypanosoma brucei

2000 ◽  
Vol 276 (15) ◽  
pp. 11559-11566 ◽  
Author(s):  
Roya Zoraghi ◽  
Stefan Kunz ◽  
Kewei Gong ◽  
Thomas Seebeck
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Trypanosoma Brucei ◽  
Dictyostelium Discoideum ◽  
Cyclic Nucleotide ◽  
Catalytic Domain ◽  
Sequence Identity ◽  
Essential Enzyme ◽  
Identification And Characterization

This study reports the identification and characterization of a cAMP-specific phosphodiesterase from the parasitic hemoflagellateTrypanosoma brucei. TbPDE2A is a class I phosphodiesterase. Its catalytic domain exhibits 30–40% sequence identity with those of all 11 mammalian phosphodiesterase (PDE) families, as well as withPDE2fromSaccharomyces cerevisiae,duncefromDrosophila melanogaster, andregAfromDictyostelium discoideum. The overall structure of TbPDE2A resembles that of human PDE11A in that its N-terminal region contains a single GAF domain. This domain is very similar to those of the mammalian PDE2, -5, -6, -10, and -11, where it constitutes a potential cGMP binding site. TbPDE2A can be expressed inS. cerevisiae, and it complements anS. cerevisiaePDE deletion strain. Recombinant TbPDE2A is specific for cAMP, with aKmof ∼2 μm. It is entirely resistant to the nonselective PDE inhibitor 3-isobutyl-1-methylxanthine, but it is sensitive to trequinsin, dipyridamole, sildenafil, and ethaverine with IC50values of 5.4, 5.9, 9.4, and 14.2 μm, respectively. All four compounds inhibit proliferation of bloodstream form trypanosomes in culture, indicating that TbPDE2A is an essential enzyme.

scholarly journals Functional Characterization of the Cyclic Nucleotide-Gated Channel SthK

2018 ◽  
Vol 114 (3) ◽  
pp. 129a
Author(s):  
Xiaolong Gao ◽  
Philipp A.M. Schmidpeter ◽  
Crina M. Nimigean
Keyword(s):  
Cyclic Nucleotide ◽  
Functional Characterization ◽  
Gated Channel

scholarly journals Functional Characterization of Gating in a Bacterial Cyclic Nucleotide-Gated Channel

2019 ◽  
Vol 116 (3) ◽  
pp. 108a
Author(s):  
Jacob Morgan ◽  
Eric Evans ◽  
William Zagotta
Keyword(s):  
Cyclic Nucleotide ◽  
Functional Characterization ◽  
Gated Channel

Molecular Cloning and Characterization of Human PDE8B, a Novel Thyroid-Specific Isozyme of 3′,5′-Cyclic Nucleotide Phosphodiesterase

1998 ◽  
Vol 250 (3) ◽  
pp. 751-756 ◽  
Author(s):  
Masaaki Hayashi ◽  
Kazuhide Matsushima ◽  
Hiroshi Ohashi ◽  
Hiroshi Tsunoda ◽  
Sumio Murase ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cyclic Nucleotide ◽  
Cyclic Nucleotide Phosphodiesterase
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