scholarly journals Detection of cryptosporidia and Cryptosporidium parvum oocysts in environmental water samples by immunomagnetic separation-polymerase chain reaction

2000 ◽  
Vol 89 (1) ◽  
pp. 5-10 ◽  
Author(s):  
S. Hallier-Soulier ◽  
E. Guillot
Keyword(s):  
Polymerase Chain Reaction ◽  
Cryptosporidium Parvum ◽  
Water Samples ◽  
Environmental Water ◽  
Immunomagnetic Separation ◽  
Environmental Water Samples ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cryptosporidium Parvum Oocysts

Direct Detection of Cryptosporidium parvum Oocysts by Immunomagnetic Separation–Polymerase Chain Reaction in Raw Milk

2002 ◽  
Vol 65 (8) ◽  
pp. 1345-1348 ◽  
Author(s):  
A. DI PINTO ◽  
M. G. TANTILLO
Keyword(s):  
Polymerase Chain Reaction ◽  
Cryptosporidium Parvum ◽  
Direct Detection ◽  
Protozoan Parasite ◽  
Raw Milk ◽  
Immunomagnetic Separation ◽  
Enteric Infection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Food Processes

Cryptosporidium parvum is an emerging protozoan parasite responsible for several serious outbreaks of cryptosporidiosis, an enteric infection characterized by severe intestinal distress. This parasite can be transmitted through contaminated water and raw food in the oocyst form, which is resistant to many environmental stresses and food processes. C. parvum is also commonly found on dairy farms and could be transmitted to humans through contaminated raw milk and dairy products. Thus, an immunomagnetic separation–polymerase chain reaction assay for direct detection of C. parvum oocysts in milk was developed. The procedure was able to detect <10 C. parvum oocysts. Thus, it could be used for monitoring milk samples.

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