Identification and characterization of a Streptococcus pyogenes ABC transporter with multiple specificity for metal cations

1999 ◽  
Vol 34 (3) ◽  
pp. 596-606 ◽  
Author(s):  
Robert Janulczyk ◽  
Jan Pallon ◽  
Lars Bjorck
Keyword(s):  
Abc Transporter ◽  
Streptococcus Pyogenes ◽  
Metal Cations ◽  
Identification And Characterization

scholarly journals Identification and Characterization of a Streptococcus pyogenes Operon Involved in Binding of Hemoproteins and Acquisition of Iron

2003 ◽  
Vol 71 (3) ◽  
pp. 1042-1055 ◽  
Author(s):  
Christopher S. Bates ◽  
Griselle E. Montañez ◽  
Charles R. Woods ◽  
Rebecca M. Vincent ◽  
Zehava Eichenbaum
Keyword(s):  
Hydrogen Peroxide ◽  
Streptococcus Pyogenes ◽  
Iron Acquisition ◽  
Iron Complex ◽  
Surface Proteins ◽  
Leader Peptide ◽  
Hemoglobin Binding ◽  
Membrane Bound ◽  
Identification And Characterization

ABSTRACT The hemolytic Streptococcus pyogenes can use a variety of heme compounds as an iron source. In this study, we investigate hemoprotein utilization by S. pyogenes. We demonstrate that surface proteins contribute to the binding of hemoproteins to S. pyogenes. We identify an ABC transporter from the iron complex family named sia for streptococcal iron acquisition, which consists of a lipoprotein (siaA), membrane permease (siaB), and ATPase (siaC). The sia transporter is part of a highly conserved, iron regulated, 10-gene operon. SiaA, which was localized to the cell membrane, could specifically bind hemoglobin. The operon's first gene encodes a novel bacterial protein that bound hemoglobin, myoglobin, heme-albumin, and hemoglobin-haptoglobin (but not apo-haptoglobin) and therefore was named Shr, for streptococcal hemoprotein receptor. PhoZ fusion and Western blot analysis showed that Shr has a leader peptide and is found in both membrane-bound and soluble forms. An M1 SF370 strain with a polar mutation in shr was more resistant to streptonigrin and hydrogen peroxide, suggesting decreased iron uptake. The addition of hemoglobin to the culture medium increased cell resistance to hydrogen peroxide in SF370 but not in the mutant, implying the sia operon may be involved in hemoglobin-dependent resistance to oxidative stress. The shr mutant demonstrated reduced hemoglobin binding, though cell growth in iron-depleted medium supplemented with hemoglobin, whole blood, or ferric citrate was not affected, suggesting additional systems are involved in hemoglobin utilization. SiaA and Shr are the first hemoprotein receptors identified in S. pyogenes; their possible role in iron capture is discussed.

scholarly journals Changes in epidemiologic characteristics and antimicrobial resistance of Streptococcus pyogenes isolated over 10 years from Japanese children with pharyngotonsillitis

2020 ◽  
Vol 69 (3) ◽  
pp. 443-450
Author(s):  
Kimiko Ubukata ◽  
Takeaki Wajima ◽  
Miyuki Morozumi ◽  
Megumi Sakuma ◽  
Takeshi Tajima ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Antibiotic Susceptibility ◽  
Rapid Identification ◽  
Specific Sequence ◽  
Content Type ◽  
Group A ◽  
Sequence Types ◽  
Identification And Characterization ◽  
Epidemiologic Characteristics

Introduction. Pharyngotonsillitis caused by Streptococcus pyogenes (group A streptococci, or GAS) is among the most common infections treated with antibiotics in pediatric patients. Aim. This study aimed to analyse changes in molecular epidemiology and antibiotic susceptibility among GAS isolates in three study periods spanning 10 years. Methodology. GAS isolated from paediatric patients with pharyngotonsillitis during Period I (mid-2007 to 2008, n=235), Period II (2012, n=210), and Period III (2018, n=189) were analysed for emm type, multilocus sequence type (MLST), antibiotic susceptibility, and macrolide (ML)- and quinolone (QL)-resistance genes. Results. Over 20 % of isolates represented emm1 and emm12 types, remaining common in all three periods. Among other emm types, emm4 was common in Period I, emm28 and emm89 in Period II, and emm3 and emm89 in Period III. All isolates remained highly susceptible to penicillins and cephalosporins. Isolates possessing mefA, ermA, or ermB genes mediating ML resistance increased from 34.9 % in Period I to 60.9 % in Period II, but fell to 27.5 % in Period III. QL-resistant isolates with amino acid substitutions affecting ParC and/or GyrA gradually increased from 11.5 to 14.3 %. Specific sequence types identified by MLST and emm typing were associated closely with ML or QL resistance. Conclusion. Our findings indicate that even in ambulatory care, antibiotic choice for these infections should be based on rapid identification and characterization of causative pathogens.

Identification and characterization of an iron ABC transporter operon in Gluconacetobacter diazotrophicus Pal 5

2013 ◽  
Vol 195 (6) ◽  
pp. 431-438 ◽  
Author(s):  
Lucia Soto Urzúa ◽  
Ada P. Vázquez-Candanedo ◽  
Adriana Sánchez-Espíndola ◽  
Carlos Ávila Ramírez ◽  
Beatriz E. Baca
Keyword(s):  
Abc Transporter ◽  
Gluconacetobacter Diazotrophicus ◽  
Identification And Characterization
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