Isolation of an RNA-dependent RNA Polymerase from Nicotiana benthamiana Plants Infected with Tobacco Necrosis Virus (strain D) and Template-dependent Transcription of its Satellite, Satellite Tobacco Necrosis Virus (strain C) RNA

2001 ◽  
Vol 149 (3-4) ◽  
pp. 167-169 ◽  
Author(s):  
Coutts ◽  
Plante
Keyword(s):  
Rna Polymerase ◽  
Virus Strain ◽  
Nicotiana Benthamiana ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus ◽  
Rna Dependent Rna Polymerase

scholarly journals RNA-Dependent RNA Polymerase 1 from Nicotiana tabacum Suppresses RNA Silencing and Enhances Viral Infection in Nicotiana benthamiana

2010 ◽  
Vol 22 (4) ◽  
pp. 1358-1372 ◽  
Author(s):  
Xiao-Bao Ying ◽  
Li Dong ◽  
Hui Zhu ◽  
Cheng-Guo Duan ◽  
Quan-Sheng Du ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Rna Polymerase ◽  
Viral Infection ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Rna Dependent Rna Polymerase ◽  
Rna Polymerase 1

Development and Optimization of Tobacco necrosis virus A Induced Gene Silencing in Nicotiana benthamiana*

2011 ◽  
Vol 38 (10) ◽  
pp. 919-928 ◽  
Author(s):  
Yang GAO ◽  
Yong-Liang ZHANG ◽  
Xiao-Feng ZHANG ◽  
Cheng-Gui HAN ◽  
Jia-Lin YU ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

RNA polymerase from tobacco necrosis virus-infected and uninfected tobacco

Virology ◽  
1977 ◽  
Vol 81 (2) ◽  
pp. 224-236 ◽  
Author(s):  
C. Stussi-Garaud ◽  
J. Lemius ◽  
H. Fraenkel-Conrat
Keyword(s):  
Rna Polymerase ◽  
Tobacco Necrosis Virus ◽  
Necrosis Virus

scholarly journals Analysis of tombusvirus revertants to identify essential amino acid residues within RNA-dependent RNA polymerase motifs

2005 ◽  
Vol 86 (3) ◽  
pp. 823-826 ◽  
Author(s):  
K. Boonrod ◽  
S. Chotewutmontri ◽  
D. Galetzka ◽  
G. Krczal
Keyword(s):  
Rna Polymerase ◽  
Nicotiana Benthamiana ◽  
Essential Amino Acid ◽  
Selection Pressure ◽  
Rna Binding ◽  
Amino Acid Residues ◽  
Rna Dependent Rna Polymerase ◽  
Viral Rdrp ◽  
In Trans ◽  
Residue Position

The RNA-dependent RNA polymerase (RdRp) of Tomato bushy stunt virus (TBSV) contains an arginine- and proline-rich (RPR) motif. This motif functions as an RNA-binding domain and is essential for tombusvirus replication. A mutant carrying three arginine substitutions in this motif rendered the virus unable to replicate in Nicotiana benthamiana plants and protoplasts. When the replicase function was provided in trans, by expressing the TBSV RdRp in N. benthamiana plants, an infectious variant could be isolated. Sequence analysis showed that only the substituted glycine residue (position 216) had reverted to arginine; all other substitutions remained unchanged. This finding suggested that strong selection pressure is active to maintain necessary sequences of the viral RdRp and that the analysis of revertants may help to identify essential viral functions.

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