Phyllodes tumors of the breast: Correlation of nucleolar organizer regions with histopathological malignancy grading, flow cytometric DNA analysis and clinical outcome

2001 ◽  
Vol 51 (11) ◽  
pp. 866-873 ◽  
Author(s):  
Hemamali Samaratunga ◽  
Belinda Clarke ◽  
Leigh Owen ◽  
Greg Bryson ◽  
Cheryl Swanson
Keyword(s):  
Clinical Outcome ◽  
Dna Analysis ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Phyllodes Tumors ◽  
Flow Cytometric ◽  
Malignancy Grading

Flow cytometric DNA analysis of neuroblastoma. Correlation with histology and clinical outcome

Cancer ◽  
1986 ◽  
Vol 58 (11) ◽  
pp. 2453-2458 ◽  
Author(s):  
Ted Gansler ◽  
Jane Chatten ◽  
Michael Varello ◽  
Greta R. Bunin ◽  
BARBARA Atkinson
Keyword(s):  
Clinical Outcome ◽  
Dna Analysis ◽  
Flow Cytometric

Nucleolar organizer regions (NORs) and myoepitheliomas: a comparison with DNA content and clinical course

1992 ◽  
Vol 106 (7) ◽  
pp. 616-620 ◽  
Author(s):  
Rosario Carrillo ◽  
Adel K. El-Naggar ◽  
Mario A. Luna ◽  
Jose L. Rodriguez-Peralto ◽  
John G. Batsakis
Keyword(s):  
Salivary Gland ◽  
Dna Content ◽  
Clinical Course ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Redundant Information ◽  
Flow Cytometric ◽  
Clinicopathologic Parameters

AbstractNucleolar organizer regions (NOR) were studied in 15 salivary gland myoepitheliomas by an argyrophilic staining technic (AgNOR). The AgNOR data were then compared with flow cytometric DNA content of the neoplasms and also with selected clinicopathologic parameters. We conclude that AgNOR's: (1) do not correlate well with DNA cytometric indices and (2) at best, provide redundant information.

The Importance of the Eosinophil in Head and Neck Cancer

1992 ◽  
Vol 106 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Manning M. Goldsmith ◽  
Deborah A. Belchis ◽  
David H. Cresson ◽  
Walter D. Merritt ◽  
Frederic B. Askin
Keyword(s):  
Head And Neck Cancer ◽  
Clinical Outcome ◽  
Head And Neck ◽  
Neck Cancer ◽  
Prognostic Indicator ◽  
High Grade ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Malignancy Grading ◽  
Grading Scales

In a previous study, we found tumor-associated tissue eosinophilia (TATE) to be a favorable prognostic indicator for squamous cell carcinoma of the head and neck ( p < 0.05). The present expanded study was undertaken to confirm this finding. The pathology of 120 head and neck tumors was examined for histologic features suggestive of poor prognosis. Ten descriptive histopathologic variables, including two malignancy grading scales, were correlated with DNA flow cytometric data and clinical outcome. No correlation was found between the malignancy grading scales and DNA flow cytometric data or clinical outcome. The present expanded study confirmed with greater statistical significance ( p < 0.001) that high-grade TATE is a favorable prognostic indicator for head and neck cancer. Furthermore, high-grade TATE was associated with the absence of distant metastasis ( p < 0.05). Using a stepwise logistic regression analysis of the clinicopathologic variables in the study, high-grade TATE was the most influential variable affecting clinical outcome, followed by border, stage, and perineural invasion. We conclude that the grade of TATE is a significant prognostic indicator for head and neck cancer. The significance and possible role of the eosinophil in the tumor–host interaction are discussed.

Electron Microscopic investigation of crooke’s change in the pituitaries of patients with hypercorticism

1989 ◽  
Vol 47 ◽  
pp. 848-849
Author(s):  
E. Horvath ◽  
K. Kovacs ◽  
L. Stefaneanu ◽  
N. Losinski
Keyword(s):  
Nucleolar Organizer ◽  
Microscopic Investigation ◽  
Electron Microscopic Investigation ◽  
Ectopic Acth Syndrome ◽  
Nucleolar Organizer Regions ◽  
Electron Microscopic ◽  
Human Pituitary ◽  
Ectopic Acth ◽  
Crooke’S Hyalinization

Human pituitary corticotropins have unique morphologic markers: bundles of type-1 filaments, measuring approximately 70 A in width and representing cytokeratin. The extreme ring-like accumulation of type-1 filaments, known as Crooke's hyalinization, signals functional suppression of the corticotropins and occurs in endogenous and exogenous glucocorticoid excess, caused by ACTH-secreting pituitary adenoma, glucocorticoid secreting adrenocortical tumor, ectopic ACTH-syndrome and administration of pharmacologic doses of glucocorticoids. Cells of autonomous corticotroph adenomas usually do not show Crooke's hyalin change. A minority of these tumors, however, retains sensitivity to the negative feed-back effect of elevated blood glucocorticoid levels and display typical Crooke’s change.In the present study pituitary corticotropins in various phases of Crooke's hyalinization were investigated in patients with glucocorticoid excess of various origin, applying histology, immunocytochemistry, count of argyrophilic nucleolar organizer regions (AgNOR), and transmission electron microscopy.

A role for upstream binding factor in organizing ribosomal gene chromatin

2006 ◽  
Vol 73 ◽  
pp. 77-84 ◽  
Author(s):  
Jane E. Wright ◽  
Christine Mais ◽  
José-Luis Prieto ◽  
Brian McStay
Keyword(s):  
Nucleolar Organizer ◽  
Ribosomal Gene ◽  
Rna Polymerase I ◽  
Nucleolar Organizer Regions ◽  
Binding Factor ◽  
Upstream Binding Factor ◽  
Acrocentric Chromosomes ◽  
Polymerase I ◽  
Secondary Constrictions ◽  
Active Nors

Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I transcription factor UBF (upstream binding factor) binds extensively across the ribosomal gene repeat throughout the cell cycle. Evidence that UBF underpins NOR structure is provided by an examination of cell lines in which large arrays of a heterologous UBF binding sequences are integrated at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus, and during metaphase form novel silver-stainable secondary constrictions, termed pseudo-NORs, that are morphologically similar to NORs.

DNA analysis and clinical outcome in pediatric adrenal cortical tumors

Pathology ◽  
1993 ◽  
Vol 25 (2) ◽  
pp. 144-147 ◽  
Author(s):  
L. Moore ◽  
R.W. Byard ◽  
N.H. Bramwell
Keyword(s):  
Clinical Outcome ◽  
Dna Analysis

Structure and variability of nucleolar organizer regions in Parodontidae fish

1984 ◽  
Vol 26 (5) ◽  
pp. 564-568 ◽  
Author(s):  
Orlando Moreira-Filho ◽  
Luiz Antonio Carlos Bertollo ◽  
Pedro Manoel Galetti Jr.
Keyword(s):  
Chromosome Pair ◽  
Constitutive Heterochromatin ◽  
Nucleolar Organizer ◽  
Nucleolar Organizer Regions ◽  
Mitotic Chromosomes ◽  
Homozygous Condition ◽  
Nucleolar Organizing Regions ◽  
Nucleolar Chromosome

Nucleolar organizer regions (NORs) were studied in mitotic chromosomes of four species of fish of family Parodontidae: Parodon tortuosus, Apareiodon affinis, Apareiodon ibitiensis, and Apareiodon piracicabae. All four species exhibited only a single nucleolar chromosome pair in their karyotypes. Intraspecific differences were observed in the size of these chromosomes; however, these were not very clear for A. affinis and A. piracicabae, Apareiodon piracicabae exhibited two clearly visible NORs in each of the nucleolar chromosomes, which was the only configuration practically found in this species. This trait therefore predominates in a homozygous condition in the population investigated. Regions of constitutive heterochromatin adjacent to the two NORs were detected. Possible mechanisms that may have originated the two NORs are discussed.Key words: nucleolar organizing regions, fish.

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