Flow cytometric analysis of localized adenocarcinoma of the prostate: The use of archival DNA analysis in conjunction with pathological grading to predict clinical outcome following radical retropubic prostatectomy

The Prostate ◽  
1990 ◽  
Vol 17 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Kenneth S. Ring ◽  
Frieda S. Karp ◽  
Carl A. Olsson ◽  
Kathleen O'Toole ◽  
Ronda Bixon ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Dna Analysis ◽  
Flow Cytometric Analysis ◽  
Radical Retropubic Prostatectomy ◽  
Retropubic Prostatectomy ◽  
Flow Cytometric ◽  
Adenocarcinoma Of The Prostate ◽  
Archival Dna

scholarly journals Myeloid cell dynamics correlate with clinical outcomes of severe coronavirus disease 2019

2020 ◽  
Author(s):  
Tomohiro Takano ◽  
Takayuki Matsumura ◽  
Yu Adachi ◽  
Kazutaka Terahara ◽  
Saya Moriyama ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Flow Cytometric Analysis ◽  
Myeloid Cell ◽  
Suppressor Cell ◽  
Recovery Phase ◽  
Cell Compartment ◽  
Cell Dynamics ◽  
Flow Cytometric ◽  
Myeloid Derived Suppressor Cell ◽  
Plasma Cytokines

Abstract An expanded myeloid cell compartment is a hallmark of severe coronavirus disease 2019 (COVID-19); however, it remains unclear whether myeloid cells are beneficial or detrimental to the clinical outcome. Here, we tracked cellular dynamics of myeloid-derived suppressor cell (MDSC) subsets and examined whether any of them correlate with disease severity and prognosis by flow cytometric analysis of blood samples from COVID-19 patients. We observed that polymorphonuclear (PMN)-MDSCs, rather than other MDSC subsets, transiently expanded in severe cases but not in mild or moderate cases. Notably, this subset was selectively expanded in survivors of severe cases and diminished during recovery. Analysis of plasma cytokines/chemokines revealed that interleukin-8 increased prior to PMN-MDSC expansion in survivors and returned to basal levels during the recovery phase. In contrast, interleukin-6 and interferon--induced protein 10 were abundantly induced in non-survivors, suggesting possible downstream targets for the immunosuppressive effects of the MDSC subset. Our data indicate that increased cellularity of PMN-MDSCs might be beneficial for the clinical outcome and could be useful as a possible predictor of prognosis in cases of severe COVID-19.

Flow cytometric DNA analysis of neuroblastoma. Correlation with histology and clinical outcome

Cancer ◽  
1986 ◽  
Vol 58 (11) ◽  
pp. 2453-2458 ◽  
Author(s):  
Ted Gansler ◽  
Jane Chatten ◽  
Michael Varello ◽  
Greta R. Bunin ◽  
BARBARA Atkinson
Keyword(s):  
Clinical Outcome ◽  
Dna Analysis ◽  
Flow Cytometric

scholarly journals Myeloid cell dynamics correlate with clinical outcomes of severe coronavirus disease 2019

2020 ◽  
Author(s):  
Tomohiro Takano ◽  
Takayuki Matsumura ◽  
Yu Adachi ◽  
Kazutaka Terahara ◽  
Saya Moriyama ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Flow Cytometric Analysis ◽  
Myeloid Cell ◽  
Suppressor Cell ◽  
Recovery Phase ◽  
Cell Compartment ◽  
Cell Dynamics ◽  
Flow Cytometric ◽  
Myeloid Derived Suppressor Cell ◽  
Plasma Cytokines

Abstract An expanded myeloid cell compartment is a hallmark of severe coronavirus disease 2019 (COVID-19); however, it remains unclear whether myeloid cells are beneficial or detrimental to the clinical outcome. Here, we tracked cellular dynamics of myeloid-derived suppressor cell (MDSC) subsets and examined whether any of them correlate with disease severity and prognosis by flow cytometric analysis of blood samples from COVID-19 patients. We observed that polymorphonuclear (PMN)-MDSCs, rather than other MDSC subsets, transiently expanded in severe cases but not in mild or moderate cases. Notably, this subset was selectively expanded in survivors of severe cases and diminished during recovery. Analysis of plasma cytokines/chemokines revealed that interleukin-8 increased prior to PMN-MDSC expansion in survivors and returned to basal levels during the recovery phase. In contrast, interleukin-6 and interferon-g-induced protein 10 were abundantly induced in non-survivors, suggesting possible downstream targets for the immunosuppressive effects of the MDSC subset. Our data indicate that increased cellularity of PMN-MDSCs might be beneficial for the clinical outcome and could be useful as a possible predictor of prognosis in cases of severe COVID-19.

The prediction of recurrence in meningiomas

1989 ◽  
Vol 71 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Paul L. May ◽  
John C. Broome ◽  
John Lawry ◽  
Robert A. Buxton ◽  
Robert D. E. Battersby
Keyword(s):  
Dna Analysis ◽  
Flow Cytometric Analysis ◽  
World Health ◽  
Proliferative Index ◽  
Content Type ◽  
Flow Cytometric ◽  
Flow Cytometric Study ◽  
Health Organization ◽  
Fine Print

✓ Despite the complete macroscopic excision of meningiomas, there is a significant rate of recurrence approaching 20% at 20 years. The prediction of recurrence by clinical and histopathological means is inadequate. Flow cytometric analysis of deoxyribonucleic acid (DNA) in meningiomas has shown a correlation between a high proliferative index based on tumor cell-cycle stage (%S + %G2/M) and clinically aggressive behavior. Accordingly, the DNA analysis of meningioma tissue may be of value in predicting recurrence of these tumors. To test this hypothesis, the DNA of paraffin-embedded archival tissue from known recurrent meningiomas was compared with an age- and sex-matched nonrecurrent group. Both groups had comparable follow-up periods. Forty patients with total macroscopic removal at the time of surgery were analyzed. The paraffin blocks of these tumors were retrieved and reclassified histologically according to the World Health Organization system. Sections were then taken for flow cytometric study. The DNA analysis showed that the proliferative index of the recurrent group was significantly higher than that of the nonrecurrent group (p < 0.002), although the histological subtyping of the two groups was similar. These results support the suggestion that flow cytometry may be of value in the prediction of recurrence of histologically benign, macroscopically removed meningiomas.

scholarly journals Myeloid cell dynamics correlate with clinical outcomes of severe coronavirus disease 2019

2020 ◽  
Author(s):  
Tomohiro Takano ◽  
Takayuki Matsumura ◽  
Yu Adachi ◽  
Kazutaka Terahara ◽  
Saya Moriyama ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Flow Cytometric Analysis ◽  
Myeloid Cell ◽  
Suppressor Cell ◽  
Recovery Phase ◽  
Cell Compartment ◽  
Cell Dynamics ◽  
Flow Cytometric ◽  
Myeloid Derived Suppressor Cell ◽  
Plasma Cytokines

Abstract An expanded myeloid cell compartment is a hallmark of severe coronavirus disease 2019 (COVID-19); however, it remains unclear whether myeloid cells are beneficial or detrimental to the clinical outcome. Here, we tracked cellular dynamics of myeloid-derived suppressor cell (MDSC) subsets and examined whether any of them correlate with disease severity and prognosis by flow cytometric analysis of blood samples from COVID-19 patients. We observed that polymorphonuclear (PMN)-MDSCs, rather than other MDSC subsets, transiently expanded in severe cases but not in mild or moderate cases. Notably, this subset was selectively expanded in survivors of severe cases and diminished during recovery. Analysis of plasma cytokines/chemokines revealed that interleukin-8 increased prior to PMN-MDSC expansion in survivors and returned to basal levels during the recovery phase. In contrast, interleukin-6 and interferon--induced protein 10 were abundantly induced in non-survivors, suggesting possible downstream targets for the immunosuppressive effects of the MDSC subset. Our data indicate that increased cellularity of PMN-MDSCs might be beneficial for the clinical outcome and could be useful as a possible predictor of prognosis in cases of severe COVID-19.

Maximum Androgen Deprivation prior to Radical Retropubic Prostatectomy in Patients with Stage T(3) Adenocarcinoma of the Prostate

1995 ◽  
Vol 28 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Gunther E. Voges ◽  
Alexandre M. Mottrie ◽  
Jan Fichtner ◽  
Christoph Mappes ◽  
Stephan Störkel ◽  
...  
Keyword(s):  
Radical Retropubic Prostatectomy ◽  
Androgen Deprivation ◽  
Retropubic Prostatectomy ◽  
Adenocarcinoma Of The Prostate
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