Recombinant Human Erythropoietin Stimulates Production of Interleukin 2 by Whole Blood Cell Cultures of Hemodialysis Patients

The production of the cytokines IFN-gamma, IL-2, IL-1-alpha and TNF-alpha was investigated in the supernatants of mitogen-stimulated whole blood cell cultures from 52 oral contraceptive users, 33 pregnant women and 75 female controls. Cells from women taking oral contraceptives produced significantly less IFN-gamma (p≤0.05) and TNF-alpha (p≤0.05) compared to the controls. In the cultures of the pregnant women significantly lower levels of IFN-gamma (p≤0.01), IL-2 (p ≤0.001) and TNF-alpha (p≤0.01) were found than in those of the controls. Total and differential leukocyte counts were almost identical in all three groups of women. It is suggested, that lymphocyte and monocyte function as measured by cytokine production is altered in women taking oral contraceptives and in pregnant women.

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Impaired cytokine production in whole blood cell cultures of patients with gynaecological carcinomas in different clinical stages

British Journal of Cancer ◽

10.1038/bjc.1993.282 ◽

1993 ◽

Vol 68 (1) ◽

pp. 32-36 ◽

Cited By ~ 24

Author(s):

U Elsässer-Beile ◽

S von Kleist ◽

W Sauther ◽

H Gallati ◽

J Schulte Mönting

Keyword(s):

Blood Cell ◽

Cell Cultures ◽

Whole Blood ◽

Cytokine Production ◽

Clinical Stages ◽

Whole Blood Cell Cultures

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Cytokine Production (IL-6 and TNFa) in Whole Blood Cell Cultures of Patients with Systemic Lupus Erythematosus

Scandinavian Journal of Rheumatology ◽

10.3109/03009749609069992 ◽

1996 ◽

Vol 25 (4) ◽

pp. 233-238 ◽

Cited By ~ 29

Author(s):

A. J. G. Swaak ◽

H. G. Van Den Brink ◽

L. A. Aarden

Keyword(s):

Systemic Lupus Erythematosus ◽

Blood Cell ◽

Cell Cultures ◽

Lupus Erythematosus ◽

Whole Blood ◽

Cytokine Production ◽

Systemic Lupus ◽

Whole Blood Cell Cultures

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Immunomodulatory Effects of Antipsychotic Drugs in Whole Blood Cell Cultures from Healthy Subjects

Current Psychiatry Research and Reviews ◽

10.2174/2666082215666191018160333 ◽

2020 ◽

Vol 15 (4) ◽

pp. 261-266

Author(s):

Eun-Jeong Kim ◽

Yong-Ku Kim

Keyword(s):

Blood Cell ◽

Cell Cultures ◽

Whole Blood ◽

Antipsychotic Drugs ◽

Future Research ◽

Immunomodulatory Effects ◽

In Vitro Production ◽

Therapeutic Concentration ◽

Whole Blood Cell Cultures

Objective: We aimed to evaluate the effects of various antipsychotics on the in vitro production of C-reactive protein (CRP) in whole blood cell cultures from healthy volunteers. The evaluation was performed using haloperidol, quetiapine, clozapine, amisulpride, and chlorpromazine. Methods: Antipsychotic agents were added to the participants' whole blood samples, and the resulting CRP levels were measured. For each agent, three different concentrations were tested: the therapeutic concentration, one-tenth the therapeutic concentration, and ten times the therapeutic concentration. The differences in CRP concentrations before and after drug administration were investigated. Results: The Friedman test showed that haloperidol, amisulpride, and chlorpromazine significantly increased CRP levels in the blood culture samples; however, clozapine and quetiapine did not increase CRP levels. In the case of chlorpromazine, elevated CRP levels were noted at all concentrations tested. Conclusion: Our study suggests that some antipsychotics elevate CRP levels in vitro. These results agree with previous studies showing that antipsychotics have immunomodulatory effects. Future research will clarify our findings and our understanding of antipsychotic drugs and their impact on immune regulation.

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