Practicality and accuracy of prehospital rapid venous blood glucose determination

2000 ◽  
Vol 18 (6) ◽  
pp. 690-694 ◽  
Author(s):  
Andreas Holstein ◽  
Detlef Kühne ◽  
Hans-Georg Elsing ◽  
Ekkart Thiessen ◽  
Armin Plaschke ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Venous Blood ◽  
Glucose Determination ◽  
Blood Glucose Determination

Effect of Addition of WZB117 as an Inhibitor of Glucose Transporter 1 for Venous Blood Glucose Determination

2020 ◽  
Author(s):  
Lei Zhang ◽  
Yaqiong Ran ◽  
Yan Zhu ◽  
Qianna Zhen
Keyword(s):  
Blood Glucose ◽  
Glucose Level ◽  
Glucose Transporter ◽  
Venous Blood ◽  
Sugar Transport ◽  
Glucose Transporter 1 ◽  
Glucose Determination ◽  
Glucose Levels ◽  
Significant Difference ◽  
Blood Glucose Determination

Abstract Objective Sodium fluoride (NaF) has been applied to inhibit glycolysis in venous specimens for decades. However, it has had little effect on the rate of glycolysis in the first 1 to 2 hours, resulting in a decrease of glucose, so a more efficient method is needed. Recently, we discovered that WZB117, a specific Glut1 inhibitor, restricts glycolysis by inhibiting the passive sugar transport of human red blood cells and cancer cells. The purpose of this study was to evaluate the results of intravenous blood glucose determination after the addition of WZB117. Methods Venous specimens from 40 pairs of healthy volunteers were collected for several days and placed in tubes containing NaF plus EDTA-disodium (Na2) without WZB117 (the A group); citric acid, trisodium citrate, and EDTA-Na2 without WZB117 (B group); and NaF plus EDTA-Na2 with WZB117 (C group). The glucose concentration was measured after venipuncture and compared with test tubes treated for 1 hour, 2 hours, and 3 hours before centrifugation. Glucose level was determined by the hexokinase method. The paired t-test was used to examine differences in glucose values at baseline and at different time points. The number of misdiagnoses and the misdiagnosis rate were calculated at 2 diagnostic stages: high risk of diabetes (glucose level of 6.1 mmol/L) and diagnosis of diabetes (glucose level of 7.0 mmol/L). Results Glucose levels decreased by 1.0% at 1 hour and by 2.1% at 3 hours in the C group tubes and simultaneously decreased by 1.7% at 1 hour and by 2.5% at 3 hours in the B group tubes. In contrast, glucose levels decreased by 4.1% at 1 hour and by 6.3% at 3 hours in the A group tubes. There was a statistically significant difference in glucose levels measured in the A group tubes and B group tubes at 1 hour, 2 hours, and 3 hours. The misdiagnosis rate of clinical diagnosis in diabetes was highest in the A group tubes (7.0‰ at 1 hour, 0.1‰ at 3 hours at 7.0 mmol/L point; 14.6‰ at 1 hour, 0.4‰ at 3 hours at 6.1 mmol/L point) and lowest in the C group tubes (2.95‰ at 1 hour, 0‰ at 3 hours at 7.0 mmol/L point; 4.8‰ at 1 hour, 0.1‰ at 3 hours at 6.1 mmol/L point). Conclusion The tube addition of WZB117 is more suitable for minimizing glycolysis and has no effect on glucose levels even if specimens are left uncentrifuged for up to 3 hours.

scholarly journals Effect of Addition of Sodium Citrate in Plasma Extraction for Venous Blood Glucose Determination

2014 ◽  
Vol 05 (11) ◽  
pp. 625-629
Author(s):  
Tiago Tizziani ◽  
Diogo Alexandre Siebert ◽  
Tassiana Pancotte ◽  
Caio Mauricio Mendes de Cordova
Keyword(s):  
Blood Glucose ◽  
Sodium Citrate ◽  
Venous Blood ◽  
Glucose Determination ◽  
Blood Glucose Determination ◽  
Plasma Extraction

Progress of MIR Non-Invasive Blood Glucose Determination and Effect of Stratum Corneum

2011 ◽  
Vol 31 (9) ◽  
pp. 0900105
Author(s):  
陈星旦 Chen Xingdan ◽  
王动民 Wang Dongmin ◽  
卢启鹏 Lu Qipeng ◽  
丁海泉 Ding Haiquan
Keyword(s):  
Blood Glucose ◽  
Stratum Corneum ◽  
Glucose Determination ◽  
Non Invasive ◽  
Blood Glucose Determination

Blood Glucose Determination without Deproteinization, with Use of o-Toluidine in Dilute Acetic Acid

1971 ◽  
Vol 17 (5) ◽  
pp. 440-441 ◽  
Author(s):  
Giovanni Ceriotti
Keyword(s):  
Acetic Acid ◽  
Blood Glucose ◽  
Acid Solution ◽  
Acetic Acid Solution ◽  
Glucose Determination ◽  
Blood Glucose Determination

Abstract A method is described for determining glucose in 25 µl of plasma. o-Toluidine is used in weak acetic acid solution. Addition of the emulsifier "Cremophor" obviates the need for deproteinization. The reaction is complete after 15 min at 100°C, and the color is stable for longer than 24 h. The blank is almost colorless. The reagent is stable, colorless, not viscous, and convenient to handle.

Enhancing calibration models for non-invasive near-infrared spectroscopical blood glucose determination

1997 ◽  
Vol 359 (1) ◽  
pp. 78-82 ◽  
Author(s):  
C. Fischbacher ◽  
K.-U. Jagemann ◽  
K. Danzer ◽  
U. A. Müller ◽  
L. Papenkordt ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Near Infrared ◽  
Glucose Determination ◽  
Non Invasive ◽  
Calibration Models ◽  
Blood Glucose Determination

ERRORS IN BLOOD GLUCOSE DETERMINATION

The Lancet ◽  
1985 ◽  
Vol 325 (8424) ◽  
pp. 341 ◽  
Author(s):  
E. Sanders ◽  
M. Deadman
Keyword(s):  
Blood Glucose ◽  
Glucose Determination ◽  
Blood Glucose Determination
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