Antisecretory factor in the human cochlea and saccus endolymphaticus

A 2-D finite element model of human cochlea is established in this paper. This model includes the structure of oval window, round window, basilar membrane and cochlear duct which is filled with fluid. The basilar membrane responses are calculated with sound input on the oval window membrane. In order to study the effects of helicotrema on basilar membrane response, three different helicotrema dimensions are set up in the FE model. A two-way fluid-structure interaction numerical method is used to compute the responses in the cochlea. The influence of the helicotrema is acquired and the frequency selectivity of the basilar membrane motion along the cochlear duct is predicted. These results agree with the experiments and indicate much better results are obtained with appropriate helicotrema size.

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Identification of flotillin-1 as an interacting protein for antisecretory factor

Regulatory Peptides ◽

10.1016/j.regpep.2007.11.005 ◽

2008 ◽

Vol 146 (1-3) ◽

pp. 303-309 ◽

Cited By ~ 8

Author(s):

Ewa Johansson ◽

Ingela Jonson ◽

Mattias Bosaeus ◽

Eva Jennische

Keyword(s):

Interacting Protein ◽

Antisecretory Factor

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Molecular cloning and expression of rat antisecretory factor and its intracellular localization

Biochemistry and Cell Biology ◽

10.1139/o99-043 ◽

1999 ◽

Vol 77 (3) ◽

pp. 223-228 ◽

Cited By ~ 8

Author(s):

Kayoko Tateishi ◽

Yoshio Misumi ◽

Yukio Ikehara ◽

Kyoko Miyasaka ◽

Akihiro Funakoshi

Keyword(s):

Pituitary Gland ◽

Intestinal Mucosa ◽

Intracellular Localization ◽

Cloning And Expression ◽

Cdna Libraries ◽

Cdna Clones ◽

Cytoplasmic Protein ◽

Rat Pituitary ◽

26S Protease ◽

Antisecretory Factor

Antisecretory factor (AF) was identified as a pituitary protein that inhibits the intestinal fluid secretion induced by cholera toxin. One aim of this study was to elucidate whether AF is also synthesized in the intestine or if AF produced in the pituitary is transported to the intestinal tract for its function there. cDNA clones encoding a protein proposed to be AF were isolated from rat pituitary gland and intestinal mucosa cDNA libraries. The nucleotide sequences of clones isolated from the rat pituitary gland and intestinal mucosa were identical. The deduced amino acid sequence was highly homologous to the sequence for subunit 5a of the human 26S protease that exists abundantly in the cytosol and nucleus. The production of AF in the intestine was confirmed by Northern blot and immunoblot analyses. Immunocytochemical observations of cells transfected with the rat AF cDNA showed that the AF protein was localized in the cytoplasm. These findings suggest that the protein proposed to be AF may be a cytoplasmic protein, it exists in the intestine rather than being transported from the pituitary gland, and it may function in intestinal cells.Key words: rat antisecretory factor, 26S protease, S5a, cytoplasmic protein.

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