DNA Restriction-Enzyme Analysis of Herpes Simplex Virus Isolates Obtained from Patients with Encephalitis

1982 ◽  
Vol 307 (17) ◽  
pp. 1060-1062 ◽  
Author(s):  
Richard Whitley ◽  
Alfred D. Lakeman ◽  
Andre Nahmias ◽  
Bernard Roizman
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Restriction Enzyme ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Dna Restriction ◽  
Simplex Virus ◽  
Virus Isolates

Factors affecting recovery of latent herpes simplex virus from human trigeminal ganglia

1982 ◽  
Vol 28 (1) ◽  
pp. 123-129 ◽  
Author(s):  
M. E. Lewis ◽  
K. G. Warren ◽  
V. M. Jeffrey ◽  
T. K. Shnitka
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Surface Area ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Trigeminal Ganglia ◽  
Factors Affecting ◽  
Two Factors ◽  
Simplex Virus

The rate of recovery of herpes simplex virus (HSV) from human trigeminal ganglia explant monolayers is affected by two factors: (1) time elapsed from the death of an individual to the establishment of in vitro culture of ganglia and (2) surface area onto which ganglia are explanted. Spontaneous reactivation of HSV from human trigeminal ganglia can be maximized when ganglia are obtained within 12 h of death and explanted onto a surface area of 250 cm2. Viruses isolated by explantation of human trigeminal ganglia were found to be uniformly HSV type 1 by restriction enzyme analysis.

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