Short Communication: Cloning of the red kangaroo (Macropus rufus) follicle stimulating hormone beta subunit

1998 ◽  
Vol 10 (3) ◽  
pp. 289 ◽  
Author(s):  
Katherine Belov ◽  
Gavan A. Harrison ◽  
Desmond W. Cooper
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Follicle Stimulating Hormone ◽  
Hormonal Control ◽  
Beta Subunit ◽  
Brushtail Possum ◽  
Coding Region ◽  
Red Kangaroo ◽  
Antigenic Sites ◽  
Stimulating Hormone

The cDNA encoding the follicle stimulating hormone beta subunit (FSH-β) was isolated from a red kangaroo pituitary cDNA library by using a porcine probe and the nucleotide sequence for the coding region was determined. The highest degree of deduced amino acid sequence identity (91%) was observed between the red kangaroo and another marsupial, the brushtail possum (Trichosurus vulpecula), followed by eutherian species (76%, 75% and 74%, respectively, for pig, mouse and sheep). Based on the deduced red kangaroo FSH-β amino acid sequence, putative antigenic sites have been identified that may prove useful for studying the hormonal control of reproduction in marsupials.

The follicle-stimulating hormone β-subunit gene of the common brushtail possum (Trichosurus vulpecula): analysis of cDNA sequence and expression

1997 ◽  
Vol 9 (8) ◽  
pp. 795 ◽  
Author(s):  
Stephen B. Lawrence ◽  
Dominique M. Vanmontfort ◽  
David J. Tisdall ◽  
Kenneth P. McNatty ◽  
Andrew E. Fidler
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cdna Sequence ◽  
Follicle Stimulating Hormone ◽  
Evolutionary Conservation ◽  
Brushtail Possum ◽  
Predicted Amino Acid Sequence ◽  
Trichosurus Vulpecula ◽  
Β Subunit ◽  
Subunit Gene

Reverse transcription–PCR has been used to obtain a cDNA sequence from the follicle-stimulating hormone (FSH) β-subunit gene of the Australian brushtail possum (Trichosurus vulpecula). Comparisons of the possum FSHβ-mRNA coding region nucleotide sequence with that of six eutherian mammal homologues reveals a mean percent identity of 77·3% and 76·8% at the nucleotide and predicted amino acid-sequence levels respectively. Furthermore, the predicted amino acid sequence of the possum FSHβ mature protein shows evolutionary conservation of twelve cysteine residues and two potential N-linked glycosylation sites. The protein lacks the CAGY motif present in most reported glycoprotein β-subunit sequences. The translation termination codon and consensus polyadenylation sequence overlap, a feature observed in other mammalian FSHβ genes. Northern hybridization of total RNA from adult female possum pituitary revealed three hybridizing transcripts of approximately 2·8, 1·2 and 0·5 kb which may arise from utilizing alternative polyadenylation signals. In situ hybridization localized the FSHβ transcripts to a sub-population of anterior pituitary cells interpreted as being gonadotropes. In summary the results indicate considerable evolutionary conservation of the structure of the FSH b-subunit gene between the marsupial and eutherian mammalian lineages.

scholarly journals Complete amino acid sequence of an integrin beta subunit (beta 7) identified in leukocytes

1991 ◽  
Vol 266 (17) ◽  
pp. 11009-11016 ◽  
Author(s):  
D.J. Erle ◽  
C. Rüegg ◽  
D. Sheppard ◽  
R. Pytela
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Beta Subunit ◽  
Complete Amino Acid Sequence
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