STUDIES ON THE FORMATION OF TOBACCO MOSAIC VIRUS RIBONUCLEIC ACID, VIII. FATE OF PARENTAL VIRAL RNA IN THE HOST CELL

Abstract Infection of tobacco protoplasts with RNA of tobacco mosaic virus and the bromoviruses has been studied. The quality of the RNA was very important. Ribonuclease activity was controlled by incorporating 5 mᴍ ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus RNA but infection with TMV RNA was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral RNA examined.

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Location of Unique Sequences in Tobacco Mosaic Virus Ribonucleic Acid

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)34191-2 ◽

1968 ◽

Vol 243 (13) ◽

pp. 3671-3674

Author(s):

S Mandeles

Keyword(s):

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Ribonucleic Acid

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Virus reconstitution and the proof of the existence of genomic RNA

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.1999.0409 ◽

1999 ◽

Vol 354 (1383) ◽

pp. 583-586 ◽

Cited By ~ 13

Author(s):

H. Fraenkel-Conrat ◽

B. Singer

Keyword(s):

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Biological Properties ◽

Viral Rna ◽

Historical Overview ◽

Genomic Rna ◽

Primary Finding ◽

Work Done ◽

Component Protein

This paper is a historical overview of the work done on the tobacco mosaic virus. The primary finding was that a virus is capable of reassembling itself from its component protein and RNA, and that only the RNA carries the genomic capability of the virus. This was followed by detailed studies of the chemical and biological properties of viral RNA.

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Challenging the Role of Microtubules in Tobacco Mosaic Virus Movement by Drug Treatments Is Disputable

Journal of Virology ◽

10.1128/jvi.00453-06 ◽

2006 ◽

Vol 80 (13) ◽

pp. 6712-6715 ◽

Cited By ~ 25

Author(s):

Mark Seemanpillai ◽

Rabab Elamawi ◽

Christophe Ritzenthaler ◽

Manfred Heinlein

Keyword(s):

Tobacco Mosaic Virus ◽

Mosaic Virus ◽

Movement Protein ◽

Viral Rna ◽

Virus Movement ◽

Direct Role ◽

Microtubule Inhibitors ◽

Microtubule Polymerization ◽

Drug Treatments

ABSTRACT The movement protein (MP) of Tobacco mosaic virus interacts with microtubules during infection. Although this interaction is correlated with the function of MP in the cell-to-cell transport of viral RNA, a direct role of microtubules in the movement process was recently challenged by studies involving the treatment of plants with inhibitors of microtubule polymerization. Here, we report evidence suggesting that such treatments may not efficiently disrupt all microtubules. Thus, results obtained from studies using microtubule inhibitors may have to remain open to interpretation with regard to the involvement of microtubules in viral RNA trafficking.

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