scholarly journals The active immunoglobulin kappa chain gene is packaged by non-ubiquitin-conjugated nucleosomes.

1986 ◽  
Vol 83 (11) ◽  
pp. 3738-3742 ◽  
Author(s):  
S. Y. Huang ◽  
M. B. Barnard ◽  
M. Xu ◽  
S. Matsui ◽  
S. M. Rose ◽  
...  
Keyword(s):  
Chain Gene ◽  
Immunoglobulin Kappa ◽  
Kappa Chain

scholarly journals Silencing of the expression of the immunoglobulin kappa gene in non-B cells

1991 ◽  
Vol 11 (3) ◽  
pp. 1431-1437
Author(s):  
J W Pierce ◽  
A M Gifford ◽  
D Baltimore
Keyword(s):  
T Cells ◽  
B Cells ◽  
Cell Types ◽  
Chain Gene ◽  
Nf Kappa B ◽  
Activated T Cells ◽  
Independent Manner ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
B Lineage

Although the activating factor NF-kappa B can be present in the nucleus of many cell types, transcription and rearrangement of the immunoglobulin kappa chain gene is restricted to cells of the B lineage. Part of this specificity is determined by sequences within the major intron of the kappa gene that specifically silence gene expression in non-B cells (T cells and HeLa cells). These sequences are found in a 232-bp fragment located 5' of the NF-kappa B binding sequence of the enhancer. When this fragment is added back upstream of an active NF-kappa B site, it specifically decreases the expression of a linked gene by more than 10-fold in activated T cells but it has no effect on expression in B cells. The kappa silencer region acts in an orientation- and distance-independent manner and appears to be composed of multiple negative elements. The kappa silencer may act to restrict transcription and rearrangement of the C kappa locus to cells of the B lineage.

scholarly journals Recent duplication and germ-line diversification of rat immunoglobulin kappa chain gene joining segments.

1982 ◽  
Vol 79 (19) ◽  
pp. 5993-5997 ◽  
Author(s):  
Y. Burstein ◽  
A. V. Breiner ◽  
C. R. Brandt ◽  
C. Milcarek ◽  
R. W. Sweet ◽  
...  
Keyword(s):  
Germ Line ◽  
Chain Gene ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
Recent Duplication

scholarly journals Lack of feedback inhibition of V kappa gene rearrangement by productively rearranged alleles.

1991 ◽  
Vol 173 (2) ◽  
pp. 409-415 ◽  
Author(s):  
K Harada ◽  
H Yamagishi
Keyword(s):  
Gene Rearrangement ◽  
Feedback Inhibition ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
Double Recombination ◽  
Circular Dnas ◽  
Reading Frames ◽  
Multiple Recombination

Circular DNAs excised by immunoglobulin kappa chain gene rearrangements were cloned and characterized. 16 of 17 clones examined were double recombination products containing a V kappa-J kappa rearrangement (coding joint) as well as the reciprocal element (signal joint) of another V kappa-J kappa rearrangement. These products suggested multiple recombination, primary inversion, and secondary excision. In primary events, 5 of 16 translational reading frames were in-phase. Thus, V kappa gene rearrangement may not be inhibited by the presence of a productively rearranged allele. An unusually large trinucleotide (P) insertion forming a palindrome of 12 nucleotides was also observed in one of the coding joints.

scholarly journals Silencing of the expression of the immunoglobulin kappa gene in non-B cells.

1991 ◽  
Vol 11 (3) ◽  
pp. 1431-1437 ◽  
Author(s):  
J W Pierce ◽  
A M Gifford ◽  
D Baltimore
Keyword(s):  
T Cells ◽  
B Cells ◽  
Cell Types ◽  
Chain Gene ◽  
Nf Kappa B ◽  
Activated T Cells ◽  
Independent Manner ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
B Lineage

Although the activating factor NF-kappa B can be present in the nucleus of many cell types, transcription and rearrangement of the immunoglobulin kappa chain gene is restricted to cells of the B lineage. Part of this specificity is determined by sequences within the major intron of the kappa gene that specifically silence gene expression in non-B cells (T cells and HeLa cells). These sequences are found in a 232-bp fragment located 5' of the NF-kappa B binding sequence of the enhancer. When this fragment is added back upstream of an active NF-kappa B site, it specifically decreases the expression of a linked gene by more than 10-fold in activated T cells but it has no effect on expression in B cells. The kappa silencer region acts in an orientation- and distance-independent manner and appears to be composed of multiple negative elements. The kappa silencer may act to restrict transcription and rearrangement of the C kappa locus to cells of the B lineage.

Transposition of two different intracisternal A particle elements into an immunoglobulin kappa-chain gene

1984 ◽  
Vol 4 (12) ◽  
pp. 2565-2572
Author(s):  
R G Hawley ◽  
M J Shulman ◽  
N Hozumi
Keyword(s):  
Primer Binding Site ◽  
Chain Gene ◽  
Direct Repeats ◽  
Long Terminal Repeats ◽  
Single Nucleotide ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
Trna Primer ◽  
Terminal Repeats ◽  
Intracisternal A Particle

Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats upon insertion. In contrast, the other IAP element was not flanked by direct repeats and was missing a single nucleotide from its 3' terminus. Sequence analysis of the latter IAP element demonstrated that its long terminal repeats were not identical. Nevertheless, the long terminal repeats were organized like proviral long terminal repeats, and this IAP element did contain two regions that were analogous to retroviral priming sites for RNA-directed DNA synthesis. The region that corresponded to a retroviral tRNA primer binding site was complementary to the 3' ends of all mammalian phenylalanine tRNAs. These findings are discussed in the context of the presumed mode of transposition of IAP elements involving the reverse transcription of IAP RNA.

scholarly journals Transposition of two different intracisternal A particle elements into an immunoglobulin kappa-chain gene.

1984 ◽  
Vol 4 (12) ◽  
pp. 2565-2572 ◽  
Author(s):  
R G Hawley ◽  
M J Shulman ◽  
N Hozumi
Keyword(s):  
Primer Binding Site ◽  
Chain Gene ◽  
Direct Repeats ◽  
Long Terminal Repeats ◽  
Single Nucleotide ◽  
Immunoglobulin Kappa ◽  
Kappa Chain ◽  
Trna Primer ◽  
Terminal Repeats ◽  
Intracisternal A Particle

Each of two severely defective mouse kappa-chain genes has acquired a different intracisternal A particle (IAP) element within one of its introns. One IAP element generated 6-base-pair direct repeats upon insertion. In contrast, the other IAP element was not flanked by direct repeats and was missing a single nucleotide from its 3' terminus. Sequence analysis of the latter IAP element demonstrated that its long terminal repeats were not identical. Nevertheless, the long terminal repeats were organized like proviral long terminal repeats, and this IAP element did contain two regions that were analogous to retroviral priming sites for RNA-directed DNA synthesis. The region that corresponded to a retroviral tRNA primer binding site was complementary to the 3' ends of all mammalian phenylalanine tRNAs. These findings are discussed in the context of the presumed mode of transposition of IAP elements involving the reverse transcription of IAP RNA.

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