scholarly journals Cl- permeability of human sweat duct cells monitored with fluorescence-digital imaging microscopy: evidence for reduced plasma membrane Cl- permeability in cystic fibrosis.

1989 ◽  
Vol 86 (24) ◽  
pp. 10166-10170 ◽  
Author(s):  
S. J. Ram ◽  
K. L. Kirk
Keyword(s):  
Cystic Fibrosis ◽  
Plasma Membrane ◽  
Digital Imaging ◽  
Sweat Duct ◽  
Digital Imaging Microscopy ◽  
Human Sweat ◽  
Duct Cells ◽  
Human Sweat Duct ◽  
Cl Permeability

Regulation of Cl- permeability in normal and cystic fibrosis sweat duct cells

1990 ◽  
Vol 259 (5) ◽  
pp. C842-C846 ◽  
Author(s):  
S. J. Ram ◽  
M. L. Weaver ◽  
K. L. Kirk
Keyword(s):  
Cystic Fibrosis ◽  
Digital Imaging ◽  
Sweat Duct ◽  
Dependent Protein Kinase ◽  
Duct Cells ◽  
Dependent Protein ◽  
Regulatory Defect ◽  
Cells Cultured ◽  
Permeability Regulation ◽  
Cl Permeability

Reabsorptive cells of the human sweat gland normally exhibit a high basal Cl- permeability but are markedly impermeable to Cl- in cystic fibrosis (CF). We examined the possibility that the reduced basal Cl- permeability of CF sweat duct cells in primary culture is due to a defective regulation of plasma membrane Cl- permeability by prostaglandin E2 (PGE2), which is endogenously produced by cultured sweat duct cells. The macroscopic Cl- permeabilities of normal and CF sweat duct cells were assessed using a halide-specific fluorescent dye, 6-methoxy-N-(3-sulfopropyl)quinolinium, in combination with fluorescence digital-imaging microscopy. The Cl- and Br- permeabilities of normal sweat duct cells were markedly reduced by inhibiting endogenous PGE2 production with indomethacin. This inhibition of Cl- permeability by indomethacin was largely reversed by the addition of PGE2 (10 nM to 1 microM), but not forskolin. Conversely, PGE2 failed to stimulate the low Cl- permeabilities of sweat duct cells cultured from CF subjects. Our results support the following conclusions: 1) a defective regulation of Cl- permeability in CF is a feature of reabsorptive as well as secretory epithelial cells, and 2) the nature of this regulatory defect extends beyond altered Cl- permeability regulation by adenosine 3',5'-cyclic monophosphate-dependent protein kinase.

Formation of inositol polyphosphates in cultured human sweat duct cells in response to cholinergic stimulation

1989 ◽  
Vol 1010 (3) ◽  
pp. 352-356 ◽  
Author(s):  
Claire Doughney ◽  
Peter S. Pedersen ◽  
Margaret A. McPherson ◽  
Robert L. Dormer
Keyword(s):  
Sweat Duct ◽  
Cholinergic Stimulation ◽  
Inositol Polyphosphates ◽  
Human Sweat ◽  
Duct Cells ◽  
Human Sweat Duct

Patch-clamp study of cultured human sweat duct cells: amiloride-blockable Na+ channel

1989 ◽  
Vol 414 (3) ◽  
pp. 369-372 ◽  
Author(s):  
L. Joris ◽  
M. E. Krouse ◽  
G. Hagiwara ◽  
C. L. Bell ◽  
J. J. Wine
Keyword(s):  
Patch Clamp ◽  
Na Channel ◽  
Sweat Duct ◽  
Human Sweat ◽  
Duct Cells ◽  
Clamp Study ◽  
Human Sweat Duct
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