Correction: Structural Basis for the Nucleic Acid Binding Cooperativity of Bacteriophage T4 Gene 32 Protein: The (Lys/Arg)3(Ser/Thr)2 (LAST) Motif

1992 ◽  
Vol 89 (11) ◽  
pp. 5201-5201
Author(s):  
J. R. Casas-Finet
Keyword(s):  
Nucleic Acid ◽  
Bacteriophage T4 ◽  
Structural Basis ◽  
Nucleic Acid Binding ◽  
Gene 32 ◽  
T4 Gene 32 Protein

Quantitative analysis of the cooperativity of protein-nucleic acid interactions by electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 308-309
Author(s):  
Pamela S. Hicks ◽  
Christopher L. Andrews ◽  
David G. Bear
Keyword(s):  
Nucleic Acid ◽  
Rna Binding ◽  
Direct Method ◽  
Bacteriophage T4 ◽  
Specific Protein ◽  
Titration Data ◽  
Protein Nucleic Acid ◽  
Gene 32 ◽  
Nucleic Acid Interactions ◽  
T4 Gene 32 Protein

It is common for sing1e-stranded nucleic acid finding proteins to bind polynucleotides cooperatively. The parameter ω is used to describe the component of the association binding constant that is due to cooperative interactions between protein molecules on the polynucleotide lattice; ω is 1 for noncooperative binding, while ω can be as high as 103-104 for highly cooperative proteins such as bacteriophage T4 gene 32 protein. In the past, to has been determined by computer fitting of spectroscopic titration data. It has been suggested that electrom microscopy would provide a more direct method for estimation of the cooperativity parameter. An equation can be derived that relates the experimentally obtained average protein cluster size (at a specific protein/RNA saturation ratio) to ω. The only other parameter required for the calculation is the RNA binding site size obtained from stoichiometric titration measurements.

scholarly journals Nucleic binding affinity of bacteriophage T4 gene 32 protein in the cooperative binding mode.

1982 ◽  
Vol 257 (11) ◽  
pp. 6184-6193
Author(s):  
A M Bobst ◽  
P W Langemeier ◽  
P E Warwick-Koochaki ◽  
E V Bobst ◽  
J C Ireland
Keyword(s):  
Binding Affinity ◽  
Bacteriophage T4 ◽  
Binding Mode ◽  
Cooperative Binding ◽  
Gene 32 ◽  
T4 Gene 32 Protein

scholarly journals Structural basis of nucleic acid binding byNicotiana tabacumglycine-rich RNA-binding protein: implications for its RNA chaperone function

2014 ◽  
Vol 42 (13) ◽  
pp. 8705-8718 ◽  
Author(s):  
Fariha Khan ◽  
Mark A. Daniëls ◽  
Gert E. Folkers ◽  
Rolf Boelens ◽  
S. M. Saqlan Naqvi ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Structural Basis ◽  
Nucleic Acid Binding ◽  
Rna Chaperone ◽  
Chaperone Function
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