scholarly journals Molecular structure of a protein-tyrosine/threonine kinase activating p42 mitogen-activated protein (MAP) kinase: MAP kinase kinase.

1993 ◽  
Vol 90 (1) ◽  
pp. 173-177 ◽  
Author(s):  
J. Wu ◽  
J. K. Harrison ◽  
L. A. Vincent ◽  
C. Haystead ◽  
T. A. Haystead ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Map Kinase ◽  
Protein Tyrosine ◽  
Threonine Kinase ◽  
Protein Map ◽  
Mitogen Activated Protein ◽  
Map Kinase Kinase

scholarly journals Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.

1993 ◽  
Vol 13 (8) ◽  
pp. 4679-4690 ◽  
Author(s):  
D L Charest ◽  
G Mordret ◽  
K W Harder ◽  
F Jirik ◽  
S L Pelech
Keyword(s):  
Myelin Basic Protein ◽  
Map Kinase ◽  
Map Kinases ◽  
Basic Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Protein Tyrosine ◽  
Mitogen Activated Protein ◽  
Erk1 Protein ◽  
Map Kinase Kinase

p44erk1 is a member of a family of tyrosyl-phosphorylated and mitogen-activated protein (MAP) kinases that participate in cell cycle control. A full-length erk1 cDNA was isolated from a human hepatoma cell line (Hep G2) library. The erk1 cDNA clone shared approximately 96% predicted amino acid identity with partial sequences of rodent erk1 cognates, and the erk1 gene was assigned to human chromosome 16 by hybrid panel analysis. Human erk1 expressed in Escherichia coli as a glutathione S-transferase fusion (GST-Erk1) protein was substantially phosphorylated on tyrosine in vivo. It underwent further autophosphorylation in vitro (up to 0.01 mol of P per mol) at the regulatory Tyr-204 site and at additional tyrosine and serine residues. Threonine autophosphorylation, presumably at the regulatory Thr-202 site, was also detected weakly when the recombinant kinase was incubated in the presence of manganese, but not in the presence of magnesium. Before and after cleavage of the GST-Erk1 protein with thrombin, it exhibited a relatively high level of myelin basic protein phosphotransferase activity, which could be reduced eightfold by treatment of the kinase with the protein-tyrosine phosphatase CD45, but not by treatment with the protein-serine/threonine phosphatase 2A. The protein-tyrosine kinase p56lck catalyzed phosphorylation of GST-Erk1 at two autophosphorylations sites, including Tyr-204, and at a novel site. A further fivefold stimulation of the myelin basic protein phosphotransferase activity of the GST-Erk1 was achieved in the presence of a partially purified MAP kinase kinase from sheep platelets. Under these circumstances, there was primarily an enhancement of the tyrosine phosphorylation of GST-Erk1. This MAP kinase kinase also similarly phosphorylated a catalytically compromised version of GST-Erk1 in which Lys-71 was converted to Ala by site-directed mutagenesis.

scholarly journals The Mitogen-activated Protein (MAP) Kinase p38 and Its Upstream Activator MAP Kinase Kinase 6 Are Involved in the Activation of Signal Transducer and Activator of Transcription by Hyperosmolarity

1999 ◽  
Vol 274 (42) ◽  
pp. 30222-30227 ◽  
Author(s):  
Johannes G. Bode ◽  
Petros Gatsios ◽  
Stephan Ludwig ◽  
Ulf R. Rapp ◽  
Dieter Häussinger ◽  
...  
Keyword(s):  
Map Kinase ◽  
Signal Transducer ◽  
Protein Map ◽  
Mitogen Activated Protein ◽  
Map Kinase Kinase

scholarly journals A protein factor for ras p21-dependent activation of mitogen-activated protein (MAP) kinase through MAP kinase kinase.

1993 ◽  
Vol 90 (3) ◽  
pp. 975-979 ◽  
Author(s):  
T. Itoh ◽  
K. Kaibuchi ◽  
T. Masuda ◽  
T. Yamamoto ◽  
Y. Matsuura ◽  
...  
Keyword(s):  
Map Kinase ◽  
Protein Factor ◽  
Protein Map ◽  
Mitogen Activated Protein ◽  
Ras P21 ◽  
Map Kinase Kinase

scholarly journals Dynamin Is Required for the Activation of Mitogen-activated Protein (MAP) Kinase by MAP Kinase Kinase

1999 ◽  
Vol 274 (50) ◽  
pp. 35301-35304 ◽  
Author(s):  
Onno Kranenburg ◽  
Ingrid Verlaan ◽  
Wouter H. Moolenaar
Keyword(s):  
Map Kinase ◽  
Protein Map ◽  
Mitogen Activated Protein ◽  
Map Kinase Kinase
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