scholarly journals Tissue-specific Expression and Dietary Regulation of Chimeric Mitochondrial 3-Hydroxy-3-methylglutaryl Coenzyme A Synthase/Human Growth Hormone Gene in Transgenic Mice

1996 ◽  
Vol 271 (13) ◽  
pp. 7529-7534 ◽  
Author(s):  
Dolors Serra ◽  
Cristina Fillat ◽  
Raquel Matas ◽  
Fatima Bosch ◽  
Fausto G. Hegardt
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Growth Hormone Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Dietary Regulation ◽  
Human Growth Hormone Gene

Identification of somatogenic binding sitess in liver microsomes from normal mice and transgenic mice expressing human growth hormone gene

Life Sciences ◽  
1992 ◽  
Vol 50 (9) ◽  
pp. 615-620 ◽  
Author(s):  
R.C. Aguilar ◽  
H.N. Fernández ◽  
J.M. Dellacha ◽  
R.S. Calandra ◽  
A. Bartke ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Human Growth Hormone ◽  
Liver Microsomes ◽  
Human Growth ◽  
Growth Hormone Gene ◽  
Human Growth Hormone Gene

scholarly journals Tissue-specific, developmental, hormonal, and dietary regulation of rat phosphoenolpyruvate carboxykinase-human growth hormone fusion genes in transgenic mice.

1992 ◽  
Vol 12 (3) ◽  
pp. 1007-1020 ◽  
Author(s):  
M K Short ◽  
D E Clouthier ◽  
I M Schaefer ◽  
R E Hammer ◽  
M A Magnuson ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Human Growth Hormone ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Human Growth ◽  
Fusion Genes ◽  
Specific Expression ◽  
Tissue Specific ◽  
Cis Acting ◽  
Specific Manner

The cytosolic phosphoenolpyruvate carboxykinase (PEPCK) gene is expressed in multiple tissues and is regulated in a complex tissue-specific manner. To map the cis-acting DNA elements that direct this tissue-specific expression, we made transgenic mice containing truncated PEPCK-human growth hormone (hGH) fusion genes. The transgenes contained PEPCK promoter fragments with 5' endpoints at -2088, -888, -600, -402, and -207 bp, while the 3' endpoint was at +69 bp. Immunohistochemical analysis showed that the -2088 transgene was expressed in the correct cell types (hepatocytes, proximal tubular epithelium of the kidney, villar epithelium of the small intestine, epithelium of the colon, smooth muscle of the vagina and lungs, ductal epithelium of the sublingual gland, and white and brown adipocytes). Solution hybridization of hGH mRNA expressed from the transgenes indicated that white and brown fat-specific elements are located distally (-2088 to -888 bp) and that liver-, gut-, and kidney-specific elements are located proximally (-600 to +69 bp). However, elements outside of the region tested are necessary for the correct developmental pattern and level of PEPCK expression in kidney. Both the -2088 and -402 transgenes responded in a tissue-specific manner to dietary stimuli, and the -2088 transgene responded to glucocorticoid stimuli. Thus, different tissues utilize distinct cell-specific cis-acting elements to direct and regulate the PEPCK gene.

scholarly journals Adrenocortical Zonation in Human Growth Hormone Gene (hGH) Transgenic Mice

1993 ◽  
Vol 42 (2) ◽  
pp. 237-241 ◽  
Author(s):  
Shin TANAKA ◽  
Hideaki TOJO ◽  
Akio MATSUZAWA
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Growth Hormone Gene ◽  
Human Growth Hormone Gene
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