scholarly journals Transcriptional Activity of Heat Shock Factor 1 at 37 oC Is Repressed through Phosphorylation on Two Distinct Serine Residues by Glycogen Synthase Kinase 3α and Protein Kinases Cα and Cζ

1998 ◽  
Vol 273 (29) ◽  
pp. 18640-18646 ◽  
Author(s):  
Boyang Chu ◽  
Rong Zhong ◽  
Fabrice Soncin ◽  
Mary Ann Stevenson ◽  
Stuart K. Calderwood
Keyword(s):  
Heat Shock ◽  
Protein Kinases ◽  
Transcriptional Activity ◽  
Glycogen Synthase ◽  
Heat Shock Factor ◽  
Glycogen Synthase Kinase ◽  
Heat Shock Factor 1

scholarly journals Glycogen Synthase Kinase 3β Negatively Regulates Both DNA-binding and Transcriptional Activities of Heat Shock Factor 1

2000 ◽  
Vol 275 (37) ◽  
pp. 29147-29152 ◽  
Author(s):  
Ilungo J. Xavier ◽  
Phillipe A. Mercier ◽  
Christine M. McLoughlin ◽  
Adnan Ali ◽  
James R. Woodgett ◽  
...  
Keyword(s):  
Heat Shock ◽  
Dna Binding ◽  
Glycogen Synthase ◽  
Heat Shock Factor ◽  
Glycogen Synthase Kinase ◽  
Heat Shock Factor 1 ◽  
Glycogen Synthase Kinase 3Β

scholarly journals DAXX interacts with heat shock factor 1 during stress activation and enhances its transcriptional activity

2004 ◽  
Vol 101 (12) ◽  
pp. 4100-4105 ◽  
Author(s):  
F. Boellmann ◽  
T. Guettouche ◽  
Y. Guo ◽  
M. Fenna ◽  
L. Mnayer ◽  
...  
Keyword(s):  
Heat Shock ◽  
Transcriptional Activity ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1 ◽  
Stress Activation

scholarly journals Regulation of Molecular Chaperone Gene Transcription Involves the Serine Phosphorylation, 14-3-3ε Binding, and Cytoplasmic Sequestration of Heat Shock Factor 1

2003 ◽  
Vol 23 (17) ◽  
pp. 6013-6026 ◽  
Author(s):  
XiaoZhe Wang ◽  
Nicholas Grammatikakis ◽  
Aliki Siganou ◽  
Stuart K. Calderwood
Keyword(s):  
Heat Shock ◽  
Molecular Chaperone ◽  
Nuclear Export ◽  
Cellular Proliferation ◽  
Glycogen Synthase ◽  
Specific Inhibitor ◽  
Heat Shock Factor ◽  
Serine Phosphorylation ◽  
Heat Shock Factor 1 ◽  
Erk Pathway

ABSTRACT Heat shock factor 1 (HSF1) regulates the transcription of molecular chaperone hsp genes. However, the cellular control mechanisms that regulate HSF1 activity are not well understood. In this study, we have demonstrated for the first time that human HSF1 binds to the essential cell signaling protein 14-3-3ε. Binding of HSF1 to 14-3-3ε occurs in cells in which extracellular signal regulated kinase (ERK) is activated and blockade of the ERK pathway by treatment with the specific ERK pathway inhibitor PD98059 in vivo strongly suppresses the binding. We previously showed that ERK1 phosphorylates HSF1 on serine 307 and leads to secondary phosphorylation by glycogen synthase kinase 3 (GSK3) on serine 303 within the regulatory domain and that these phosphorylation events repress HSF1. We show here that HSF1 binding to 14-3-3ε requires HSF1 phosphorylation on serines 303 and 307. Furthermore, the serine phosphorylation-dependent binding of HSF1 to 14-3-3ε results in the transcriptional repression of HSF1 and its sequestration in the cytoplasm. Leptomycin B, a specific inhibitor of nuclear export receptor CRM1, was found to reverse the cytoplasmic sequestration of HSF1 mediated by 14-3-3ε, suggesting that CRM1/14-3-3ε directed nuclear export plays a major role in repression of HSF1 by the ERK/GSK3/14-3-3ε pathway. Our experiments indicate a novel pathway for HSF1 regulation and suggest a mechanism for suppression of its activity during cellular proliferation.

Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2

2003 ◽  
Vol 303 (2) ◽  
pp. 700-706 ◽  
Author(s):  
Fabrice Soncin ◽  
Xinfeng Zhang ◽  
Boyang Chu ◽  
Xiaozhe Wang ◽  
Alexzander Asea ◽  
...  
Keyword(s):  
Heat Shock ◽  
Dna Binding ◽  
Transcriptional Activity ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1

scholarly journals c-Jun NH2-terminal Kinase Targeting and Phosphorylation of Heat Shock Factor-1 Suppress Its Transcriptional Activity

2000 ◽  
Vol 275 (24) ◽  
pp. 18210-18218 ◽  
Author(s):  
Rujuan Dai ◽  
Wojciech Frejtag ◽  
Bin He ◽  
Yan Zhang ◽  
Nahid F. Mivechi
Keyword(s):  
Heat Shock ◽  
Transcriptional Activity ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1

scholarly journals Evidence for a Mechanism of Repression of Heat Shock Factor 1 Transcriptional Activity by a Multichaperone Complex

2001 ◽  
Vol 276 (49) ◽  
pp. 45791-45799 ◽  
Author(s):  
Yongle Guo ◽  
Toumy Guettouche ◽  
Mary Fenna ◽  
Frank Boellmann ◽  
William B. Pratt ◽  
...  
Keyword(s):  
Heat Shock ◽  
Transcriptional Activity ◽  
Heat Shock Factor ◽  
Heat Shock Factor 1
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