scholarly journals A Long, Weakly Charged Actin-binding Loop Is Required for Phosphorylation-dependent Regulation of Smooth Muscle Myosin

1998 ◽  
Vol 273 (43) ◽  
pp. 27939-27944 ◽  
Author(s):  
Arthur S. Rovner
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Muscle Myosin ◽  
Binding Loop

scholarly journals Visualization of Head–Head Interactions in the Inhibited State of Smooth Muscle Myosin

1999 ◽  
Vol 147 (7) ◽  
pp. 1385-1390 ◽  
Author(s):  
Thomas Wendt ◽  
Dianne Taylor ◽  
Terri Messier ◽  
Kathleen M. Trybus ◽  
Kenneth A. Taylor
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Intramolecular Interactions ◽  
Actin Binding ◽  
Structural Basis ◽  
Binding Interface ◽  
Structural Differences ◽  
Muscle Myosin ◽  
Positively Charged ◽  
Phosphoryla Tion

The structural basis for the phosphoryla- tion-dependent regulation of smooth muscle myosin ATPase activity was investigated by forming two- dimensional (2-D) crystalline arrays of expressed unphosphorylated and thiophosphorylated smooth muscle heavy meromyosin (HMM) on positively charged lipid monolayers. A comparison of averaged 2-D projections of both forms at 2.3-nm resolution reveals distinct structural differences. In the active, thiophosphorylated form, the two heads of HMM interact intermolecularly with adjacent molecules. In the unphosphorylated or inhibited state, intramolecular interactions position the actin-binding interface of one head onto the converter domain of the second head, thus providing a mechanism whereby the activity of both heads could be inhibited.

scholarly journals Structural and actin-binding properties of the trypsin-produced HMM and S1 from gizzard smooth muscle myosin

FEBS Letters ◽  
1983 ◽  
Vol 159 (1-2) ◽  
pp. 211-216 ◽  
Author(s):  
T. Marianne-Pépin ◽  
D. Mornet ◽  
E. Audemard ◽  
R. Kassab
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Binding Properties ◽  
Muscle Myosin

scholarly journals Smooth muscle myosin mutants containing a single tryptophan reveal molecular interactions at the actin-binding interface

1998 ◽  
Vol 95 (22) ◽  
pp. 12944-12949 ◽  
Author(s):  
C. M. Yengo ◽  
P. M. Fagnant ◽  
L. Chrin ◽  
A. S. Rovner ◽  
C. L. Berger
Keyword(s):  
Smooth Muscle ◽  
Molecular Interactions ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Binding Interface ◽  
Muscle Myosin

scholarly journals Actin-induced Closure of the Actin-binding Cleft of Smooth Muscle Myosin

2002 ◽  
Vol 277 (27) ◽  
pp. 24114-24119 ◽  
Author(s):  
Christopher M. Yengo ◽  
Enrique M. De La Cruz ◽  
Lynn R. Chrin ◽  
Donald P. Gaffney ◽  
Christopher L. Berger
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Binding Cleft ◽  
Muscle Myosin

scholarly journals Localization and topography of antigenic domains within the heavy chain of smooth muscle myosin.

1985 ◽  
Vol 101 (1) ◽  
pp. 66-72 ◽  
Author(s):  
M D Schneider ◽  
J R Sellers ◽  
M Vahey ◽  
Y A Preston ◽  
R S Adelstein
Keyword(s):  
Smooth Muscle ◽  
Immunoelectron Microscopy ◽  
Heavy Chain ◽  
Muscle Development ◽  
Solid Phase ◽  
Smooth Muscles ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Heavy Meromyosin ◽  
Muscle Myosin

We have produced and characterized monoclonal antibodies that label antigenic determinants distributed among three distinct, nonoverlapping peptide domains of the 200-kD heavy chain of avian smooth muscle myosin. Mice were immunized with a partially phosphorylated chymotryptic digest of adult turkey gizzard myosin. Hybridoma antibody specificities were determined by solid-phase indirect radioimmunoassay and immunoreplica techniques. Electron microscopy of rotary-shadowed samples was used to directly visualize the topography of individual [antibody.antigen] complexes. Antibody TGM-1 bound to a 50-kD peptide of subfragment-1 (S-1) previously found to be associated with actin binding and was localized by immunoelectron microscopy to the distal aspect of the myosin head. However, there was no antibody-dependent inhibition of the actin-activated heavy meromyosin ATPase, nor was antibody TGM-1 binding to actin-S-1 complexes inhibited. Antibody TGM-2 detected an epitope of the subfragment-2 (S-2) domain of heavy meromyosin but not the S-2 domain of intact myosin or rod, consistent with recognition of a site exposed by chymotryptic cleavage of the S-2:light meromyosin junction. Localization of TGM-2 to the carboxy-terminus of S-2 was substantiated by immunoelectron microscopy. Antibody TGM-3 recognized an epitope found in the light meromyosin portion of myosin. All three antibodies were specific for avian smooth muscle myosin. Of particular interest is that antibody TGM-1, unlike TGM-3, bound poorly to homogenates of 19-d embryonic smooth muscles. This indicates the expression of different myosin heavy chain epitopes during smooth muscle development.

Actin-binding peptide from smooth muscle myosin light chain kinase

Biochemistry ◽  
1993 ◽  
Vol 32 (34) ◽  
pp. 8902-8907 ◽  
Author(s):  
Satoshi Kanoh ◽  
Masaaki Ito ◽  
Eiji Niwa ◽  
Yasushi Kawano ◽  
David J. Hartshorne
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Binding Peptide ◽  
Muscle Myosin

scholarly journals Identification of a Novel Actin Binding Motif in Smooth Muscle Myosin Light Chain Kinase

1999 ◽  
Vol 274 (41) ◽  
pp. 29433-29438 ◽  
Author(s):  
Lula Smith ◽  
Xujun Su ◽  
Pei-ju Lin ◽  
Gang Zhi ◽  
James T. Stull
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Binding Motif ◽  
Muscle Myosin

scholarly journals Studies of functional amino acid residues in smooth muscle myosin : mutagenesis of actin binding site

2000 ◽  
Vol 40 (supplement) ◽  
pp. S60
Author(s):  
H. Onishi ◽  
S. Kojima ◽  
K. Konishi ◽  
K. Katoh ◽  
K. Fujiwara ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Amino Acid ◽  
Binding Site ◽  
Smooth Muscle Myosin ◽  
Actin Binding ◽  
Amino Acid Residues ◽  
Muscle Myosin ◽  
Actin Binding Site
Sign in / Sign up

Export Citation Format

Share Document