scholarly journals Characterization of the Elongation Complex of Dengue Virus RNA Polymerase

2010 ◽  
Vol 286 (3) ◽  
pp. 2067-2077 ◽  
Author(s):  
Zhinan Jin ◽  
Jerome Deval ◽  
Kenneth A. Johnson ◽  
David C. Swinney
Keyword(s):  
Dengue Virus ◽  
Rna Polymerase ◽  
Elongation Complex ◽  
Virus Rna

Biochemical characterization of the inhibition of the dengue virus RNA polymerase by beta-d-2′-ethynyl-7-deaza-adenosine triphosphate

2010 ◽  
Vol 87 (2) ◽  
pp. 213-222 ◽  
Author(s):  
Derek R. Latour ◽  
Andreas Jekle ◽  
Hassan Javanbakht ◽  
Robert Henningsen ◽  
Peter Gee ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rna Polymerase ◽  
Adenosine Triphosphate ◽  
Biochemical Characterization ◽  
Virus Rna

scholarly journals Analysis of Ribonucleotide 5′-Triphosphate Analogs as Potential Inhibitors of Zika Virus RNA-Dependent RNA Polymerase by Using Nonradioactive Polymerase Assays

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Gaofei Lu ◽  
Gregory R. Bluemling ◽  
Paul Collop ◽  
Michael Hager ◽  
Damien Kuiper ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Zika Virus ◽  
Nonstructural Protein ◽  
Rna Dependent Rna Polymerase ◽  
Antiviral Compounds ◽  
Double Stranded Dna ◽  
Virus Rna ◽  
Potential Inhibitors

ABSTRACT Zika virus (ZIKV) is an emerging human pathogen that is spreading rapidly through the Americas and has been linked to the development of microcephaly and to a dramatically increased number of Guillain-Barré syndrome cases. Currently, no vaccine or therapeutic options for the prevention or treatment of ZIKV infections exist. In the study described in this report, we expressed, purified, and characterized full-length nonstructural protein 5 (NS5) and the NS5 polymerase domain (NS5pol) of ZIKV RNA-dependent RNA polymerase. Using purified NS5, we developed an in vitro nonradioactive primer extension assay employing a fluorescently labeled primer-template pair. Both purified NS5 and NS5pol can carry out in vitro RNA-dependent RNA synthesis in this assay. Our results show that Mn2+ is required for enzymatic activity, while Mg2+ is not. We found that ZIKV NS5 can utilize single-stranded DNA but not double-stranded DNA as a template or a primer to synthesize RNA. The assay was used to compare the efficiency of incorporation of analog 5′-triphosphates by the ZIKV polymerase and to calculate their discrimination versus that of natural ribonucleotide triphosphates (rNTPs). The 50% inhibitory concentrations for analog rNTPs were determined in an alternative nonradioactive coupled-enzyme assay. We determined that, in general, 2′-C-methyl- and 2′-C-ethynyl-substituted analog 5′-triphosphates were efficiently incorporated by the ZIKV polymerase and were also efficient chain terminators. Derivatives of these molecules may serve as potential antiviral compounds to be developed to combat ZIKV infection. This report provides the first characterization of ZIKV polymerase and demonstrates the utility of in vitro polymerase assays in the identification of potential ZIKV inhibitors.

scholarly journals Establishment and characterization of plasmid-driven minigenome rescue systems for Nipah virus: RNA polymerase I- and T7-catalyzed generation of functional paramyxoviral RNA

Virology ◽  
2008 ◽  
Vol 370 (1) ◽  
pp. 33-44 ◽  
Author(s):  
Alexander Freiberg ◽  
Lhia Krista Dolores ◽  
Sven Enterlein ◽  
Ramon Flick
Keyword(s):  
Rna Polymerase ◽  
Nipah Virus ◽  
Rna Polymerase I ◽  
Virus Rna ◽  
Polymerase I

Biochemical Characterization of Respiratory Syncytial Virus RNA-Dependent RNA Polymerase Complex

2020 ◽  
Vol 6 (10) ◽  
pp. 2800-2811
Author(s):  
Anand Balakrishnan ◽  
Edmund Price ◽  
Catherine Luu ◽  
Jacob Shaul ◽  
Charles Wartchow ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rna Polymerase ◽  
Biochemical Characterization ◽  
Rna Dependent Rna Polymerase ◽  
Polymerase Complex ◽  
Virus Rna ◽  
Syncytial Virus

scholarly journals Full-length Dengue Virus RNA-dependent RNA Polymerase-RNA/DNA Complexes

2011 ◽  
Vol 286 (38) ◽  
pp. 33095-33108 ◽  
Author(s):  
Michal R. Szymanski ◽  
Maria J. Jezewska ◽  
Paul J. Bujalowski ◽  
Cecile Bussetta ◽  
Mengyi Ye ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rna Polymerase ◽  
Full Length ◽  
Rna Dependent Rna Polymerase ◽  
Dna Complexes ◽  
Virus Rna

Production and characterization of active hepatitis C virus RNA-dependent RNA polymerase

2010 ◽  
Vol 71 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Kisun Ryu ◽  
Kyun-Hwan Kim ◽  
Seong-Yeon Yoo ◽  
Eun-Young Lee ◽  
Keo-Heun Lim ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Hepatitis C Virus Rna ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna

scholarly journals Conformational Flexibility of the Dengue Virus RNA-Dependent RNA Polymerase Revealed by a Complex with an Inhibitor

2013 ◽  
Vol 87 (9) ◽  
pp. 5291-5295 ◽  
Author(s):  
C. G. Noble ◽  
S. P. Lim ◽  
Y.-L. Chen ◽  
C. W. Liew ◽  
L. Yap ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rna Polymerase ◽  
Conformational Flexibility ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna
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