Biochemical Characterization of Respiratory Syncytial Virus RNA-Dependent RNA Polymerase Complex

2020 ◽  
Vol 6 (10) ◽  
pp. 2800-2811
Author(s):  
Anand Balakrishnan ◽  
Edmund Price ◽  
Catherine Luu ◽  
Jacob Shaul ◽  
Charles Wartchow ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rna Polymerase ◽  
Biochemical Characterization ◽  
Rna Dependent Rna Polymerase ◽  
Polymerase Complex ◽  
Virus Rna ◽  
Syncytial Virus

scholarly journals Biochemical Characterization of Enzyme Fidelity of Influenza A Virus RNA Polymerase Complex

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e10372 ◽  
Author(s):  
Shilpa Aggarwal ◽  
Birgit Bradel-Tretheway ◽  
Toru Takimoto ◽  
Stephen Dewhurst ◽  
Baek Kim
Keyword(s):  
Rna Polymerase ◽  
Influenza A Virus ◽  
Influenza A ◽  
Biochemical Characterization ◽  
Polymerase Complex ◽  
Virus Rna

scholarly journals Biochemical characterization of a hepatitis C virus RNA-dependent RNA polymerase mutant lacking the C-terminal hydrophobic sequence

2000 ◽  
Vol 81 (3) ◽  
pp. 759-767 ◽  
Author(s):  
Licia Tomei ◽  
Alessandra Vitelli ◽  
Raffaele De Francesco ◽  
Rosa Letizia Vitale ◽  
Ilario Incitti ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Biochemical Characterization ◽  
Hepatitis C Virus Rna ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna

scholarly journals Optimization of Potent and Selective Quinazolinediones: Inhibitors of Respiratory Syncytial Virus That Block RNA-Dependent RNA-Polymerase Complex Activity

2014 ◽  
Vol 57 (24) ◽  
pp. 10314-10328 ◽  
Author(s):  
Daljit S. Matharu ◽  
Daniel P. Flaherty ◽  
Denise S. Simpson ◽  
Chad E. Schroeder ◽  
Donghoon Chung ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rna Polymerase ◽  
Complex Activity ◽  
Rna Dependent Rna Polymerase ◽  
Polymerase Complex ◽  
Syncytial Virus

Intrinsic Disorder to Order Transitions in the Scaffold Phosphoprotein P from the Respiratory Syncytial Virus RNA Polymerase Complex

Biochemistry ◽  
2016 ◽  
Vol 55 (10) ◽  
pp. 1441-1454 ◽  
Author(s):  
María G. Noval ◽  
Sebastian A. Esperante ◽  
Ivana G. Molina ◽  
Lucía B. Chemes ◽  
Gonzalo de Prat-Gay
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rna Polymerase ◽  
Intrinsic Disorder ◽  
Polymerase Complex ◽  
Virus Rna ◽  
Syncytial Virus

scholarly journals Analysis of Ribonucleotide 5′-Triphosphate Analogs as Potential Inhibitors of Zika Virus RNA-Dependent RNA Polymerase by Using Nonradioactive Polymerase Assays

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Gaofei Lu ◽  
Gregory R. Bluemling ◽  
Paul Collop ◽  
Michael Hager ◽  
Damien Kuiper ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Zika Virus ◽  
Nonstructural Protein ◽  
Rna Dependent Rna Polymerase ◽  
Antiviral Compounds ◽  
Double Stranded Dna ◽  
Virus Rna ◽  
Potential Inhibitors

ABSTRACT Zika virus (ZIKV) is an emerging human pathogen that is spreading rapidly through the Americas and has been linked to the development of microcephaly and to a dramatically increased number of Guillain-Barré syndrome cases. Currently, no vaccine or therapeutic options for the prevention or treatment of ZIKV infections exist. In the study described in this report, we expressed, purified, and characterized full-length nonstructural protein 5 (NS5) and the NS5 polymerase domain (NS5pol) of ZIKV RNA-dependent RNA polymerase. Using purified NS5, we developed an in vitro nonradioactive primer extension assay employing a fluorescently labeled primer-template pair. Both purified NS5 and NS5pol can carry out in vitro RNA-dependent RNA synthesis in this assay. Our results show that Mn2+ is required for enzymatic activity, while Mg2+ is not. We found that ZIKV NS5 can utilize single-stranded DNA but not double-stranded DNA as a template or a primer to synthesize RNA. The assay was used to compare the efficiency of incorporation of analog 5′-triphosphates by the ZIKV polymerase and to calculate their discrimination versus that of natural ribonucleotide triphosphates (rNTPs). The 50% inhibitory concentrations for analog rNTPs were determined in an alternative nonradioactive coupled-enzyme assay. We determined that, in general, 2′-C-methyl- and 2′-C-ethynyl-substituted analog 5′-triphosphates were efficiently incorporated by the ZIKV polymerase and were also efficient chain terminators. Derivatives of these molecules may serve as potential antiviral compounds to be developed to combat ZIKV infection. This report provides the first characterization of ZIKV polymerase and demonstrates the utility of in vitro polymerase assays in the identification of potential ZIKV inhibitors.

scholarly journals Cryo-EM structure of the respiratory syncytial virus RNA polymerase

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Dongdong Cao ◽  
Yunrong Gao ◽  
Claire Roesler ◽  
Samantha Rice ◽  
Paul D’Cunha ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Rna Polymerase ◽  
Virus Rna ◽  
Syncytial Virus

Production and characterization of active hepatitis C virus RNA-dependent RNA polymerase

2010 ◽  
Vol 71 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Kisun Ryu ◽  
Kyun-Hwan Kim ◽  
Seong-Yeon Yoo ◽  
Eun-Young Lee ◽  
Keo-Heun Lim ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Hepatitis C Virus Rna ◽  
Rna Dependent Rna Polymerase ◽  
Virus Rna
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