Analysing microbial community structure by means of terminal restriction fragment length polymorphism (T-RFLP).

2009 ◽  
pp. 84-98 ◽  
Author(s):  
C. B. Blackwood
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Microbial Community Structure ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Restriction Fragment Length

scholarly journals Fingerprinting Microbial Assemblages from the Oxic/Anoxic Chemocline of the Black Sea

2003 ◽  
Vol 69 (11) ◽  
pp. 6481-6488 ◽  
Author(s):  
Costantino Vetriani ◽  
Hiep V. Tran ◽  
Lee J. Kerkhof
Keyword(s):  
Microbial Community ◽  
Restriction Fragment Length Polymorphism ◽  
Water Column ◽  
Black Sea ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
The Black Sea ◽  
Restriction Fragment Length

ABSTRACT Biomass samples from the Black Sea collected in 1988 were analyzed for SSU genes from Bacteria and Archaea after 10 years of storage at −80°C. Both clonal libraries and direct fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analyses were used to assess the microbial community. Uniform and discrete depth distributions of different SSU phylotypes were observed. However, most recombinant clones were not restricted to a specific depth in the water column, and many of the major T-RFLP peaks remain uncharacterized. Of the clones obtained, an ε-Proteobacteria and a Pseudoalteromonas-like clone accounted for major peaks in the fingerprint, while deeply branching lineages of α- and γ-Proteobacteria were associated with smaller peaks. Additionally, members were found among both the δ-Proteobacteria related to sulfate reducers and the Archaea related to phylotypes from the ANME groups that anaerobically oxidize methane.

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