Transformation ofAspergillus oryzaethrough Plasmid-mediated Complementation of the Methionine-auxotrophic Mutation

SummaryAlachlor, butachlor and carbaryl were highly toxic to the N2-fixing cyanobacterium Nostoc muscorum, when grown on solid minimal (nitrogen-free) medium. At pesticides doses allowing about 20, 50 and 60% survival of the organism, various mutations were obtained in N. muscorum, reverse mutation from het+nif– and het–nif+ auxotrophy to het+nif+ prototrophy, forward mutation for resistance to 0·37 mM L-methionine-DL-sulfoximine, and auxotrophic mutation for the achievement of carbon-auxotrophy through 5 mM methylamine-resistance. The toxic and mutagenic effects of the three pesticides were similar to those of the well known mutagen MNNG.

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Transformation of Aspergillus oryzae through plasmid-mediated complementation of the methionine-auxotrophic mutation.

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.51.323 ◽

1987 ◽

Vol 51 (2) ◽

pp. 323-328 ◽

Cited By ~ 23

Author(s):

Yuzuru IIMURA ◽

Katsuya GOMI ◽

Hiroshi Uzu ◽

Shodo HARA

Keyword(s):

Aspergillus Oryzae ◽

Auxotrophic Mutation

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Documentation of Auxotrophic Mutation in Blue-Green Bacteria: Characterization of a Tryptophan Auxotroph in Agmenellum quadruplicatum

Journal of Bacteriology ◽

10.1128/jb.111.1.112-118.1972 ◽

1972 ◽

Vol 111 (1) ◽

pp. 112-118 ◽

Cited By ~ 20

Author(s):

Lonnie O. Ingram ◽

Duane Pierson ◽

James F. Kane ◽

C. Van Baalen ◽

Roy A. Jensen

Keyword(s):

Green Bacteria ◽

Agmenellum Quadruplicatum ◽

Auxotrophic Mutation

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Mutation of an Arginine Biosynthesis Gene Causes Reduced Pathogenicity in Fusarium oxysporum f. sp. melonis

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2001.14.4.580 ◽

2001 ◽

Vol 14 (4) ◽

pp. 580-584 ◽

Cited By ~ 176

Author(s):

Fumio Namiki ◽

Michiko Matsunaga ◽

Mitsuru Okuda ◽

Iori Inoue ◽

Kazufumi Nishi ◽

...

Keyword(s):

Structural Analysis ◽

Fusarium Oxysporum ◽

Restriction Enzyme ◽

Argininosuccinate Lyase ◽

Arginine Biosynthesis ◽

Biosynthesis Gene ◽

Remi Mutagenesis ◽

Auxotrophic Mutation ◽

Arginine Auxotroph

Restriction enzyme-mediated integration (REMI) mutagenesis was used to tag genes required for pathogenicity of Fusarium oxysporum f. sp. melonis. Of the 1,129 REMI transformants tested, 13 showed reduced pathogenicity on susceptible melon cultivars. One of the mutants, FMMP95–1, was an arginine auxotroph. Structural analysis of the tagged site in FMMP95-1 identified a gene, designated ARG1, which possibly encodes argininosuccinate lyase, catalyzing the last step for arginine biosynthesis. Complementation of FMMP95–1 with the ARG1 gene caused a recovery in pathogenicity, indicating that arginine auxotrophic mutation causes reduced pathogenicity in this pathogen.

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