Studies on Human Hepatic Cholesterol Ester Hydrolase in Liver Disease

1978 ◽  
Vol 38 (sup150) ◽  
pp. 218-222 ◽  
Author(s):  
J. B. Simon ◽  
R. W. M. Poon
Keyword(s):  
Liver Disease ◽  
Cholesterol Ester ◽  
Hepatic Cholesterol ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase

CHOLESTEROL ESTER HYDROLYSIS BY HOMOGENATES OF WHOLE TESTIS, SEMINIFEROUS TUBULES AND INTERSTITIAL CELLS OF MATURE RATS

1977 ◽  
Vol 75 (2) ◽  
pp. 235-243 ◽  
Author(s):  
J. P. RENSTON ◽  
T. J. IHRIG ◽  
R. H. RENSTON ◽  
B. GONDOS ◽  
R. J. MORIN
Keyword(s):  
Cholesterol Ester ◽  
Incubation Temperature ◽  
Hydrolytic Activity ◽  
Interstitial Cells ◽  
Seminiferous Tubules ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Optimum Ph ◽  
Hormone Biosynthesis ◽  
Hydrolysis Of

The characteristics and localization of a cholesterol ester hydrolase enzyme in homogenates of whole testis and in isolated seminiferous tubules and interstitial cells of mature rats have been investigated. Hydrolysis of cholesteryl [1-14C]oleate occurred at an optimum pH of 7·0 was linearly related to time up to 5–6 h of incubation and increased linearly up to 0·25 mg protein/incubation. Hydrolytic activity was inhibited by increasing the incubation temperature from 29 to 41 °C and by sonication. Cholesterol ester hydrolase activity/mg protein was three times greater in homogenates of seminiferous tubules than in interstitial cells. Cholesterol ester hydrolase may function to provide precursors for use in seminiferous tubular steroid hormone biosynthesis or germ cell maturation.

scholarly journals Regulation of cholesterol ester hydrolase by cyclic AMP-dependent protein kinase

FEBS Letters ◽  
1986 ◽  
Vol 201 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Roger J. Colbran ◽  
Andrew J. Garton ◽  
Susan R. Cordle ◽  
Stephen J. Yeaman
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Cholesterol Ester ◽  
Dependent Protein Kinase ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Dependent Protein

Proposed Treatment for Infants With Wolman Disease

PEDIATRICS ◽  
1989 ◽  
Vol 83 (6) ◽  
pp. 1074-1075
Author(s):  
MOSHE WOLMAN
Keyword(s):  
Lysosomal Enzyme ◽  
Cholesterol Ester ◽  
Reticuloendothelial System ◽  
Cholesterol Esters ◽  
Enzyme Molecule ◽  
Acid Lipase ◽  
Wolman Disease ◽  
Cholesterol Ester Hydrolase ◽  
Ester Hydrolase ◽  
Available Information

Presently available information indicates that Wolman disease is due to a defect in a single lysosomal enzyme molecule, called acid lipase, esterase, or cholesterol ester hydrolase (EC3.1.1.13). Cells of the reticuloendothelial system, hepatocytes, adrenocortical, and presumably many other cells normally, contain a neutral esterase associated with their microsomes, which is not deficient in Wolman disease. Theoretically, catabolism of intracellular hydrophobic cholesterol (esters and triglycerides) should proceed normally in Wolman disease cells whenever the metabolic chain does not depend on lysosomal hydrolysis.

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