THE EFFECT OF ALGINATE ON THE INVASION OF CYSTIC FIBROSIS RESPIRATORY EPITHELIAL CELLS BY CLINICAL ISOLATES OF PSEUDOMONAS AERUGINOSA

2000 ◽  
Vol 26 (3) ◽  
pp. 163-178 ◽  
Author(s):  
A. Rene ◽  
D. Massengale ◽  
F. J. Quinn ◽  
A. Williams ◽  
S. Gallagher ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Clinical Isolates ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

scholarly journals Microarray Analysis Reveals Induction of Lipoprotein Genes in Mucoid Pseudomonas aeruginosa: Implications for Inflammation in Cystic Fibrosis

2004 ◽  
Vol 72 (9) ◽  
pp. 5012-5018 ◽  
Author(s):  
Aaron M. Firoved ◽  
Wojciech Ornatowski ◽  
Vojo Deretic
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Microarray Analysis ◽  
High Morbidity ◽  
Dependent Manner ◽  
Respiratory Epithelial Cells ◽  
Therapeutic Benefits ◽  
Human Respiratory Epithelial Cells ◽  
Respiratory Epithelial

ABSTRACT The main cause of the high morbidity and mortality of cystic fibrosis (CF) is the progressive lung inflammation associated with Pseudomonas aeruginosa colonization. During the course of chronic CF infections, P. aeruginosa undergoes a conversion to a mucoid phenotype. The emergence of mucoid P. aeruginosa in CF is associated with increased inflammation, respiratory decline, and a poor prognosis. Here we show, by the use of microarray analysis, that upon P. aeruginosa conversion to mucoidy, there is a massive and preferential induction of genes encoding bacterial lipoproteins. Bacterial lipoproteins are potent agonists of Toll-like receptor 2 (TLR2) signaling. The expression of TLR2 in human respiratory epithelial cells was ascertained by Western blot analysis. Human respiratory epithelial cells responded in a TLR2-dependent manner to bacterial lipopeptides derived from Pseudomonas lipoproteins induced in mucoid strains. The TLR2 proinflammatory response was further augmented in CF cells. Thus, the excessive inflammation in CF is the result of a global induction in mucoid P. aeruginosa of lipoproteins that act as proinflammatory toxins (here termed lipotoxins) superimposed on the hyperexcitability of CF cells. Blocking the signaling cascade responding to bacterial lipotoxins may provide therapeutic benefits for CF patients.

X-ray micro-analysis of cultured respiratory epithelial cells from patients with cystic fibrosis

1992 ◽  
Vol 146 (2) ◽  
pp. 213-220 ◽  
Author(s):  
S. SAGSTRÖM ◽  
G. M. ROOMANS ◽  
R. WROBLEWSKI ◽  
J. L. M. KEULEMANS ◽  
A. T. HOOGEVEEN ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Respiratory Epithelial Cells ◽  
X Ray ◽  
Respiratory Epithelial ◽  
Micro Analysis
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