Designing of dual biosensor system for detection of zinc and chromium from contaminated soil using saponin biosurfactant and bioluminescence bacteria

Author(s):  
Reza Hajimohammadi ◽  
Mohammad Johari -Ahar ◽  
Sajjad Ahmadpour
2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Aziz Babapoor ◽  
Reza Hajimohammadi ◽  
Seyyed Mohammad Jokar ◽  
Meysam Paar

In this study, a biosensor is designed to remove mercury as a toxic metal contaminant from the soil. The rhamnolipid biosurfactant was used to extract the mercury sorbed to soil to the aqueous phase. An immobilized bioluminescent bacterium (Escherichia coli MC106) with pmerRBPmerlux plasmid is assisted for mercury detection. A significant decrease in luminescence level was observed in a biosensor system containing contaminated soil sample extract. The concentrations of extracting mercury are well correlated with the mercury toxicity data obtained from experimental biosensor systems according to the RBL value. The optimum aeration rate of 20 ml/min was obtained for the biosensor systems. The advantage of such a biosensor is the in situ quantification of mercury as a heavy metal contaminant in soils. Therefore, this system could be proposed as a good biosensor-based alternative for future detection of heavy toxic metals in soils.


2019 ◽  
Vol 10 (3) ◽  
pp. 148
Author(s):  
Nesreen Kurdy Al-Obaidy ◽  
Assad Al-Shueli ◽  
Hawraa Sattar ◽  
Zainab Majeed ◽  
Noor Al Huda Hamid

Author(s):  
A.I. Barbashev ◽  
◽  
S.N. Sushkova ◽  
T.M. Minkina ◽  
T.S. Dudnikova ◽  
...  

The effect of diatomite and tripoli added into the soil artificially contaminated with benzo[a]pyrene was studied. A decrease in the toxic effect of BaP on barley plants was established when diatomite and tripoli were added as ameliorants to contaminated soil. The improvement of plants grown сharacteristics on soils contaminated with BaP with the addition of diatomite and tripoli was shown up to 2-4 times compare to contaminated samples.


Author(s):  
V.V. Zinchenko ◽  
◽  
E.S Fedorenko ◽  
A.V Gorovtsov ◽  
T.M Minkina ◽  
...  

As a result of the model experiment, an increase in the enzymatic activity of meadow chernozem of the impact zone of Ataman Lake with the introduction of a strains mixture of metal-resistant microorganisms into the soil was established. The experiment has shown that the application of bacterial strains increases the dehydrogenase activity of contaminated soil by 51.8% compared to the variant without remediation


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