Amino acid changes in the variable region of VP2 in three infectious bursal disease viruses with different virulence, originating from a common ancestor

Nucleotide sequences of the VP2 hypervariable region (VP2-HVR) of 10 infectious bursal disease viruses detected in indigenous and exotic chickens in Zambia from 2004 to 2005 were determined. Phylogenetic analysis showed that the viruses diverged into two genotypes and belonged to the African very virulent types (VV1 and VV2). In the phylogenetic tree, strains in one genotype clustered in a distinct group and were closely related to some strains isolated in western Africa (VV1), with nucleotide similarities of 95.7%– 96.5%. Strains in the other genotype were clustered within the eastern African VV type (VV2), with nucleotide similarities of 97.3%– 98.5%. Both genotypes were distributed in the southern parts of Zambia and had a unique conserved amino acid substitution at 300 (E→A) in addition to the putative virulence marker at positions 222(A), 242(I), 256(I), 294(I) and 299(S). These findings represent the first documentation of the existence of the African VV-IBDV variants in both indigenous and exotic chickens in Zambia.

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Spatiotemporal Phylogenetic Analysis and Molecular Characterisation of Infectious Bursal Disease Viruses Based on the VP2 Hyper-Variable Region

PLoS ONE ◽

10.1371/journal.pone.0065999 ◽

2013 ◽

Vol 8 (6) ◽

pp. e65999 ◽

Cited By ~ 15

Author(s):

Abdulahi Alfonso-Morales ◽

Orlando Martínez-Pérez ◽

Roser Dolz ◽

Rosa Valle ◽

Carmen L. Perera ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Variable Region ◽

Infectious Bursal Disease ◽

Molecular Characterisation ◽

Infectious Bursal Disease Viruses ◽

Bursal Disease ◽

Hyper Variable Region

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Molecular characterisation of infectious bursal disease viruses isolated in recent acute outbreaks in Solvenia

Acta Veterinaria Hungarica ◽

10.1556/avet.56.2008.2.13 ◽

2008 ◽

Vol 56 (2) ◽

pp. 255-264 ◽

Cited By ~ 2

Author(s):

Olga Zorman Rojs ◽

Uroš Krapež ◽

Brigita Slavec ◽

Sara Mankoč ◽

Rahela Jurišič-Cizerl ◽

...

Keyword(s):

Amino Acid ◽

Phylogenetic Analyses ◽

Hypervariable Region ◽

Infectious Bursal Disease ◽

Amino Acid Substitutions ◽

Molecular Characterisation ◽

Broiler Breeder ◽

Infectious Bursal Disease Viruses ◽

Bursal Disease ◽

Serotype 1

In 2004 and then in 2006 several outbreaks of infectious bursal disease (IBD) were reported in broiler and broiler breeder flocks in Slovenia. In this report ten recently emerged IBD viruses (IBDV) were characterised by sequence analysis of the VP2 hypervariable region and compared to previous Slovene IBDV strains from 1995/1996 and to some representative serotype 1 IBDV strains of different pathotypes. On the basis of nucleotide and amino acid identities, phylogenetic analyses and the presence of very virulent IBDV (vvIBDV) conserved amino acid substitutions, all Slovene isolates from recent outbreaks were identified as vvIBDV. Although some unique nucleotide exchanges and amino acid substitutions have been observed, the results of this study indicated that recent vvIBDV isolates are closely related with those from outbreaks in the 1990s. However, acute IBD has not been reported in commercial flocks in Slovenia for some years. This could lead to the conclusion that poor biosecurity and relaxed vaccination could be responsible for the re-emergence of vvIBDV.

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Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity

Virology ◽

10.1016/j.virol.2008.04.018 ◽

2008 ◽

Vol 377 (1) ◽

pp. 110-116 ◽

Cited By ~ 60

Author(s):

D.J. Jackwood ◽

B. Sreedevi ◽

L.J. LeFever ◽

S.E. Sommer-Wagner

Keyword(s):

Amino Acid ◽

Amino Acid Substitution ◽

Single Amino Acid Substitution ◽

Infectious Bursal Disease ◽

Single Amino Acid ◽

Naturally Occurring ◽

Infectious Bursal Disease Viruses ◽

Bursal Disease

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Amino Acid Comparison of Infectious Bursal Disease Viruses Placed in the Same or Different Molecular Groups by RT/PCR-RFLP

Avian Diseases ◽

10.2307/1592972 ◽

2001 ◽

Vol 45 (2) ◽

pp. 330 ◽

Cited By ~ 38

Author(s):

D. J. Jackwood ◽

S. E. Sommer ◽

H. V. Knoblich

Keyword(s):

Amino Acid ◽

Infectious Bursal Disease ◽

Rt Pcr ◽

Infectious Bursal Disease Viruses ◽

Pcr Rflp ◽

Amino Acid Comparison ◽

Bursal Disease ◽

Acid Comparison

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APPLICATION OF SHIFTING ASSAY OF STANDARDIZED INDIRECT AGGLUTINATION (SSIA) FOR DETECTION OF ANTIGENS OF NEWCASTLE DISEASE AND INFECTIOUS BURSAL DISEASE VIRUSES IN CHICKEN FAECES

HUE UNIVERSITY JOURNAL OF SCIENCE AGRICULTURE AND RURAL DEVELOPMENT ◽

10.26459/jard.v83i5.3079 ◽

2013 ◽

Vol 83 (5) ◽

Author(s):

Phạm Hồng Sơn ◽

Phạm Hồng Kỳ ◽

Nguyễn Thị Lan Hương ◽

Phạm Thị Hồng Hà

Keyword(s):

Newcastle Disease ◽

Winter Season ◽

Summer Season ◽

Infectious Bursal Disease ◽

Infection Rates ◽

Faecal Samples ◽

Infectious Bursal Disease Viruses ◽

Bursal Disease ◽

Long Time ◽

Higher Sensitivity

. Using the method of shifting assay of standardized indirect agglutination (SSIA), the prevalence of Newcastle disease viruses (NDV) and infectious bursal disease viruses (IBDV) in chickens reared in several districts of Thua Thien Hue province in the Spring-Summer and Fall-Winter seasons was determined. In the Spring-Summer season of 2011, about 22.3% of the chickens were infected with NDV, in which A Luoi accounted for the highest percentage of 25% of the infected chickens and Huong Thuy the lowest of 18.2%. Meanwhile, 36% of the same chickens were infected with IBDV, with the highest percentage (46.66%) also in A Luoi and the lowest (30.3%) also in Huong Thuy. The intensity of NDV infection in the Spring-Summer season in A Luoi and Phu Vang was highest (GMT = 1.45), and in Huong Thuy lowest (GMT = 1.31). In addition, in the Fall-Winter season, about 46% of the chickens were infected with NDV and 46.3% with IBDV in Huong Thuy and Phu Vang – two neighbouring districts of Hue City, in which NDV was detected in 54.4% of the chickens in Huong Thuy and 33.9% in Phu Vang. In contrast, IBDV was detected in 41.9% and 52.7% of the chickens respectively in the two districts. The infection was not inter-dependent. Methodically, although the differences in the infection rates were insignificant with the accuracy of 95%, faecal samples showed higher sensitivity in SSIA analyses for both cases of NDV and IBDV infection in comparision with mouth exudates. By SSIA method, results could be read clearly with unaided eyes for a long time after the performance, and it was also proven applicable for cases of haemagglutinating viruses if proper treatments for depletion of animal RBCs’ surface agglutinins could be applied.

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