The anti-nuclease humoral immune response of broiler chickens exposed toStaphylococcus aureus, infectious bursal disease virus and chicken anaemia virus in an experimental model for bacterial chondronecrosis and osteomyelitis

Abstract The present study was conducted to decipher the effect of silver nanoparticles conjugated marker recombinant VP2 immunogen of Infectious bursal disease virus on humoral immune response in chickens. The hypervariable VP2 gene segment of field Infectious bursal disease virus, consisting of major and minor hydrophilic loops, was amplified using reverse transcription-polymerase chain reaction. The gene segment of size 664 bp was cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated recombinant VP2 protein (rVP2, 19 kDa) was expressed in a prokaryotic expression system using Escherichia coli BL32DE3 cells. The rVP2 protein showed reactivity with specific anti VP2 chicken antibodies. The results of Western blot revealed its utility in serological diagnosis. The recombinant antigen was tested for immunogenic potential by vaccinating the chickens with and without silver nanoparticles. The rVP2 protein blended with adjuvant grade montanide oil showed a highly significant rise in serum IgY titers. The titers induced by rVP2 protein mixed with montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and silver nanoparticles than in chickens vaccinated with conventional vaccines or rVP2 protein. The results represent Infectious bursal disease virus rVP2 protein as a promising candidate for the DIVA vaccine and sero-diagnostic tool. For the first time, the current study elucidated the adjuvanticity effect of silver nanoparticles on avian Infectious bursal disease virus rVP2 vaccine potency.

Download Full-text

Comparative Efficacy and Humoral Responses of an Inactivated Infectious Bursal Disease Virus Vaccine Prepared from a Local Isolate with that of a Commercial Live Vaccine in Layer Birds

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v2i1.1932 ◽

1970 ◽

Vol 2 (1) ◽

pp. 37-38

Author(s):

MIA Begum ◽

MA Islam ◽

ATM Mahbub-E-Elahi ◽

M Rahman ◽

MER Bhuiyan

Keyword(s):

Immune Response ◽

Disease Virus ◽

Humoral Immune Response ◽

Infectious Bursal Disease Virus ◽

Live Vaccine ◽

Infectious Bursal Disease ◽

Inactivated Vaccine ◽

Humoral Immune ◽

Local Isolate ◽

Bursal Disease

The humoral immune response and efficacy of an inactivated adjuvanted infectious bursal disease virus (IBDV) vaccine prepared with a virulent local isolate was compared with a live commercial IBDV vaccine (Nobilis D78Â®, Intervet) in 20 layer birds during the period from October to November 2002. These day-old experimental birds were divided into four groups, A, B, C and D, each consisting of 5 birds. Each bird of groups A, B and C was immunized with live IBDV vaccine (Nobilis D78Â®, Intervet), live + inactivated vaccine, and inactivated IBDV vaccine, respectively at day 7, day 21 and day 28, whereas birds of group D served as unvaccinated controls. The sera of chickens vaccinated with either combined (live + inactivated) or only inactivated IBDV vaccine showed clear band of precipitation with agar gel immunodiffusion test (AGIDT) and higher antibody titre with ELISA. The protection test revealed that the experimentally prepared inactivated IBDV vaccine gave 100% protection against 80% protection in layer birds immunized with live commercial vaccine. Key words: Efficacy; humoral immune response; IBD: inactivated vaccine; live vaccine; layer birds doi: 10.3329/bjvm.v2i1.1932 Bangl. J. Vet. Med. (2004). 2 (1) : 37-38

Download Full-text