Therapeutic Effects against Tissue Necrosis of Remote Ischemic Preconditioning Combined with Human Adipose-Derived Stem Cells in Random-Pattern Skin Flap Rat Models

Adipose-derived stem cells (ADSCs) can maintain self-renewal and enhanced multidifferentiation potential through the release of a variety of paracrine factors and extracellular vesicles, allowing them to repair damaged organs and tissues. Consequently, considerable attention has increasingly been paid to their application in tissue engineering and organ regeneration. Here, we provide a comprehensive overview of the current status of ADSC preparation, including harvesting, isolation, and identification. The advances in preclinical and clinical evidence-based ADSC therapy for bone, cartilage, myocardium, liver, and nervous system regeneration as well as skin wound healing are also summarized. Notably, the perspectives, potential challenges, and future directions for ADSC-related researches are discussed. We hope that this review can provide comprehensive and standardized guidelines for the safe and effective application of ADSCs to achieve predictable and desired therapeutic effects.

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MP89-06 THERAPEUTIC EFFECTS OF ADIPOSE-DERIVED STEM CELLS-BASED MICRO-TISSUES ON ERECTILE DYSFUNCTION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

The Journal of Urology ◽

10.1016/j.juro.2016.02.2467 ◽

2016 ◽

Vol 195 (4S) ◽

Author(s):

Bicheng Yang ◽

Yu Hui ◽

Hongen Lei ◽

Ruili Guan ◽

Zhezhu Gao ◽

...

Keyword(s):

Stem Cells ◽

Erectile Dysfunction ◽

Diabetic Rats ◽

Therapeutic Effects ◽

Adipose Derived Stem Cells

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Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α

Journal of Cellular and Molecular Medicine ◽

10.1111/j.1582-4934.2011.01313.x ◽

2011 ◽

Vol 15 (12) ◽

pp. 2575-2585 ◽

Cited By ~ 51

Author(s):

Weicheng Gao ◽

Xing Qiao ◽

Shaolin Ma ◽

Lei Cui

Keyword(s):

Stem Cells ◽

Skin Flap ◽

Hypoxia Inducible Factor ◽

Adipose Derived Stem Cells ◽

Hypoxia Inducible Factor 1Α

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Improved Viability of Random Pattern Skin Flaps through the Use of Adipose-Derived Stem Cells

Plastic & Reconstructive Surgery ◽

10.1097/01.prs.0000293876.10700.b8 ◽

2008 ◽

Vol 121 (1) ◽

pp. 50-58 ◽

Cited By ~ 156

Author(s):

Feng Lu ◽

Hiroshi Mizuno ◽

Cagri A. Uysal ◽

Xiaobo Cai ◽

Rei Ogawa ◽

...

Keyword(s):

Stem Cells ◽

Random Pattern ◽

Adipose Derived Stem Cells ◽

Skin Flaps

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Improved Random Component Viability of Axial Skin Flap through the Use of Human Adipose Derived Stem Cells

Plastic & Reconstructive Surgery ◽

10.1097/01.prs.0000455399.37294.b2 ◽

2014 ◽

Vol 134 ◽

pp. 58 ◽

Cited By ~ 1

Author(s):

Chin-Jung Feng ◽

Cherng-Kang Perng ◽

Hsu Ma

Keyword(s):

Stem Cells ◽

Skin Flap ◽

Random Component ◽

Adipose Derived Stem Cells

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Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by up-regulation of angiogenesis and glycolysis

10.21203/rs.3.rs-70182/v2 ◽

2020 ◽

Author(s):

Xiang Wan ◽

Min-kai Xie ◽

Huan Xu ◽

Zi-wei Wei ◽

Hai-jun Yao ◽

...

Keyword(s):

Stem Cells ◽

Umbilical Vein ◽

Urethral Reconstruction ◽

Therapeutic Effects ◽

Adipose Derived Stem Cells ◽

Promising Alternative ◽

Hypoxia Preconditioning ◽

Novel Scaffold

Abstract Rationale: Tissue engineering is a promising alternative for urethral reconstruction, and adipose-derived stem cells (ADSCs) are widely used as seeding cells. Hypoxia preconditioning can significantly enhance the therapeutic effects of ADSCs. The low oxygen tension of postoperative wound healing is inevitable and may facilitate the nutritional function of ADSCs. This study aimed to investigate if hypoxia preconditioned ADSCs, compared to normxia preconditioned ADSCs, combined with scaffold could better promote urethral reconstruction and exploring the underlying mechanism.Methods: In vitro, paracrine cytokines and secretomes that were secreted by hypoxia- or normoxia-preconditioned ADSCs were added to cultures of human umbilical vein endothelial cells (HUVECs) to measure their functions. In vivo, hypoxia- or normoxia-preconditioned ADSCs were seeded on a porous nanofibrous scaffold for urethral repair on a defect model in rabbits.Results: The in vitro results showed that hypoxia could enhance the secretion of VEGFA by ADSCs, and hypoxia-preconditioned ADSCs could enhance the viability, proliferation, migration, angiogenesis and glycolysis of HUVECs (p < 0.05). After silencing VEGFA, angiogenesis and glycolysis were significantly inhibited (p < 0.05). The in vivo results showed that compared to normoxia-preconditioned ADSCs, hypoxia-preconditioned ADSCs combined with scaffolds led to a larger urethral lumen diameter, preserved urethral morphology and enhanced angiogenesis (p < 0.05). Conclusions: Hypoxia preconditioning of ADSCs combined with scaffold could better promote urethral reconstruction by upregulating angiogenesis and glycolysis. Hypoxia-preconditioned ADSCs combined with novel scaffold may provide a promising alternative treatment for urethral reconstruction.

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