Radiation-induced DNA Damage and Repair in Radiosensitive and Radioresistant Human Tumour Cells Measured by Field Inversion Gel Electrophoresis

1993 ◽  
Vol 63 (6) ◽  
pp. 703-713 ◽  
Author(s):  
M.F.M.A. Smeets ◽  
E.H.M. Mooren ◽  
A.C. Begg
Keyword(s):  
Dna Damage ◽  
Gel Electrophoresis ◽  
Tumour Cells ◽  
Human Tumour ◽  
Dna Damage And Repair ◽  
Human Tumour Cells ◽  
Radiation Induced ◽  
Field Inversion

scholarly journals MEPE/OF45 as a new target for sensitizing human tumour cells to DNA damage inducers

2010 ◽  
Vol 102 (5) ◽  
pp. 862-866 ◽  
Author(s):  
P Zhang ◽  
H Wang ◽  
P S N Rowe ◽  
B Hu ◽  
Y Wang
Keyword(s):  
Dna Damage ◽  
Tumour Cells ◽  
Human Tumour ◽  
Human Tumour Cells

scholarly journals Radiation-induced DNA double-strand break rejoining in human tumour cells

1995 ◽  
Vol 71 (2) ◽  
pp. 311-316 ◽  
Author(s):  
MI Núñez ◽  
M Villalobos ◽  
N Olea ◽  
MT Valenzuela ◽  
V Pedraza ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Tumour Cells ◽  
Human Tumour ◽  
Human Tumour Cells ◽  
Dna Double Strand Break ◽  
Radiation Induced

Role of p53 mutations, protein function and DNA damage for the radiosensitivity of human tumour cells

2004 ◽  
Vol 80 (1) ◽  
pp. 53-63 ◽  
Author(s):  
A. Böhnke ◽  
F. Westphal ◽  
A. Schmidt ◽  
R. A. El‐Awady ◽  
J. Dahm‐Daphi
Keyword(s):  
Dna Damage ◽  
Protein Function ◽  
Tumour Cells ◽  
P53 Mutations ◽  
Human Tumour ◽  
Human Tumour Cells

Determination of spatial patterns of DNA damage and repair in intestinal crypts by multi-cell gel electrophoresis

1996 ◽  
Vol 109 (8) ◽  
pp. 2061-2068
Author(s):  
R.A. Brooks ◽  
D.J. Winton
Keyword(s):  
Dna Damage ◽  
Gamma Radiation ◽  
Gel Electrophoresis ◽  
Topoisomerase Ii ◽  
Spatial Relationship ◽  
Dna Damage And Repair ◽  
Strand Breaks ◽  
Incremental Change ◽  
Radiation Induced ◽  
Dna Strand

We have developed a method to quantitate DNA strand breaks as a measure of DNA damage and repair in intact, isolated intestinal crypts. The assay is a modified form of the single-cell gel electrophoresis or ‘comet’ assay. By maintaining the spatial relationship between the cells we were able to characterise the repair response and the susceptibility to DNA damage of cells as a function of their position in the crypt. All cells were equally repair competent over the first 30 minutes of the repair of UV-C and gamma-radiation induced lesions. DNA damage was equally distributed following gamma-radiation but following incubation with the topoisomerase II inhibitor etoposide, damage was greater in the lower crypt with an unusual component to the comet tall which was tapered, implying an incremental change in susceptibility by cell position. This tapered component of the comet tail resolved rapidly after removal of etoposide. The pattern of damage produced by hydrogen peroxide was dose dependent with lower doses producing more strand breaks in the base of the crypt-an effect lost at higher doses. The assay has the ability to detect differences between cells in their susceptibility to DNA damage and their subsequent repair response which may vary with their proliferative or differentiative status.

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