Digestive Alkaline Proteases from the Goby (Zosterisessor ophiocephalus): Characterization and Potential Application as Detergent Additive and in the Deproteinization of Shrimp Wastes

2012 ◽  
Vol 21 (2) ◽  
pp. 118-133 ◽  
Author(s):  
Assaâd Sila ◽  
Rim Nasri ◽  
Ali Bougatef ◽  
Moncef Nasri
Keyword(s):  
Potential Application ◽  
Alkaline Proteases ◽  
Detergent Additive ◽  
Shrimp Wastes ◽  
Zosterisessor Ophiocephalus

scholarly journals Digestive Alkaline Proteases from Zosterisessor ophiocephalus, Raja clavata, and Scorpaena scrofa: Characteristics and Application in Chitin Extraction

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Rim Nasri ◽  
Islem Younes ◽  
Imen Lassoued ◽  
Sofiane Ghorbel ◽  
Olfa Ghorbel-Bellaaj ◽  
...  
Keyword(s):  
Proteolytic Enzymes ◽  
Tween 20 ◽  
Sodium Perborate ◽  
Alkaline Proteases ◽  
Shrimp Waste ◽  
Wide Range ◽  
Raja Clavata ◽  
Shrimp Wastes ◽  
Scorpaena Scrofa ◽  
Zosterisessor Ophiocephalus

The aim of this work was to study some biochemical characteristics of crude alkaline protease extracts from the viscera of goby (Zosterisessor ophiocephalus), thornback ray (Raja clavata), and scorpionfish (Scorpaena scrofa), and to investigate their applications in the deproteinization of shrimp wastes. At least four caseinolytic proteases bands were observed in zymogram of each enzyme preparation. The optimum pH for enzymatic extracts activities of Z. ophiocephalus, R. clavata, and S. scrofa were 8.0-9.0, 8.0, and 10.0, respectively. Interestingly, all the enzyme preparations were highly stable over a wide range of pH from 6.0 to 11.0. The optimum temperatures for enzyme activity were 50∘C for Z. ophiocephalus and R. clavata and 55∘C for S. scrofa crude alkaline proteases. Proteolytic enzymes showed high stability towards non-ionic surfactants (5% Tween 20, Tween 80, and Triton X-100). In addition, crude proteases of S. scrofa, R. clavata, and Z. ophiocephalus were found to be highly stable towards oxidizing agents, retaining 100%, 70%, and 66%, respectively, of their initial activity after incubation for 1 h in the presence of 1% sodium perborate. They were, however, highly affected by the anionic surfactant SDS. The crude alkaline proteases were tested for the deproteinization of shrimp waste in the preparation of chitin. All proteases were found to be effective in the deproteinization of shrimp waste. The protein removals after 3 h of hydrolysis at 45∘C with an enzyme/substrate ratio (E/S) of 10 were about 76%, 76%, and 80%, for Z. ophiocephalus, R. clavata, and S. scrofa crude proteases, respectively. These results suggest that enzymatic deproteinization of shrimp wastes by fish endogenous alkaline proteases could be applicable to the chitin production process.

Alkaline proteases and thermostable α-amylase co-produced by Bacillus licheniformis NH1: Characterization and potential application as detergent additive

2009 ◽  
Vol 47 (1-3) ◽  
pp. 71-79 ◽  
Author(s):  
Noomen Hmidet ◽  
Nedra El-Hadj Ali ◽  
Anissa Haddar ◽  
Safia Kanoun ◽  
Sellami-Kamoun Alya ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Potential Application ◽  
Alkaline Proteases ◽  
Detergent Additive

Albacore tuna spleen trypsin: Potential application as laundry detergent additive and in carotenoprotein extraction from Pacific white shrimp shells

2019 ◽  
Vol 17 ◽  
pp. 638-646 ◽  
Author(s):  
Tanchanok Poonsin ◽  
Benjamin K. Simpson ◽  
Soottawat Benjakul ◽  
Wonnop Visessanguan ◽  
Asami Yoshida ◽  
...  
Keyword(s):  
Potential Application ◽  
Laundry Detergent ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Detergent Additive ◽  
Albacore Tuna ◽  
Shrimp Shells

Trypsin from zebra blenny (Salaria basilisca) viscera: Purification, characterisation and potential application as a detergent additive

2012 ◽  
Vol 130 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Naourez Ktari ◽  
Hayet Ben Khaled ◽  
Rim Nasri ◽  
Kemel Jellouli ◽  
Sofiane Ghorbel ◽  
...  
Keyword(s):  
Potential Application ◽  
Detergent Additive

An Oxidant- and Solvent-Stable Protease Produced by Bacillus cereus SV1: Application in the Deproteinization of Shrimp Wastes and as a Laundry Detergent Additive

2009 ◽  
Vol 160 (8) ◽  
pp. 2308-2321 ◽  
Author(s):  
Laila Manni ◽  
Kemel Jellouli ◽  
Olfa Ghorbel-Bellaaj ◽  
Rym Agrebi ◽  
Anissa Haddar ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Laundry Detergent ◽  
Detergent Additive ◽  
Shrimp Wastes ◽  
Solvent Stable
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