Daily cocoa intake reduces the susceptibility of low-density lipoprotein to oxidation as demonstrated in healthy human volunteers

AbstractThe role of triglycerides carried in the triglyceride-rich lipoproteins (TRL) in the progression of atherosclerosis is uncertain. Identification of oxidized triglycerides and its possible association with atherosclerosis were largely ignored. Here we applied mass spectrometric approach to detect and identify triglyceride hydroperoxides (TGOOH) in human plasma and lipoproteins.EDTA plasma was collected from healthy human volunteers (n=9) after 14–16 h of fasting. Very low-density lipoprotein (VLDL)We identified 11 molecular species of TGOOH in either plasma or VLDL and IDL, of which TGOOH-18:1/18:2/16:0, TGOOH-18:1/18:1/16:0, TGOOH-16:0/18:2/16:0, TGOOH-18:1/18:1/18:1, and TGOOH-16:0/20:4/16:0 were most dominant. These TGOOH molecules are carried by TRL but not by LDL and HDL. Mean concentration of TGOOH in plasma, VLDL and IDL were, respectively, 56.1±25.6, 349.8±253.6 and 512.5±173.2 μmol/mol of triglycerides.This is the first report to identify several molecular species of oxidized triglycerides in TRL. Presence of oxidized triglyceride may contribute to the atherogenicity of TRL. Further work is needed to elucidate the association of the oxidized triglyceride in atherosclerosis.

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4P-0980 Efficacy of quercetin and ganhuangenin on oxidatively modified low density lipoprotein obtained from healthy human plasma

Atherosclerosis Supplements ◽

10.1016/s1567-5688(03)91238-4 ◽

2003 ◽

Vol 4 (2) ◽

pp. 289

Author(s):

B.O. Lim ◽

S.H. Sun Hee Lee ◽

I.S. Lee ◽

K.H. Park ◽

M.O. Lee ◽

...

Keyword(s):

Human Plasma ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Healthy Human ◽

Oxidatively Modified

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Effect of simvastatin on receptor-dependent low density lipoprotein catabolism in normocholesterolemic human volunteers

Atherosclerosis ◽

10.1016/0021-9150(89)90017-8 ◽

1989 ◽

Vol 80 (2) ◽

pp. 101-109 ◽

Cited By ~ 32

Author(s):

Claude L. Malmendier ◽

Jean-F. Lontie ◽

Claude Delcroix ◽

Thierry Magot

Keyword(s):

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density ◽

Human Volunteers

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Intralysosomal Accumulation of Colloidal Gold-Low Density Lipoprotein Conjugates in Chloroquine-Treated Fibroblasts

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011951x ◽

1985 ◽

Vol 43 ◽

pp. 546-547 ◽

Cited By ~ 1

Author(s):

Dean A. Handley ◽

Cynthia M. Arbeeny ◽

Larry D. Witte

Keyword(s):

Colloidal Gold ◽

Cultured Cells ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Coated Vesicle ◽

Low Density ◽

Cholesterol Esters ◽

Cultured Fibroblasts ◽

Surface Receptors ◽

Ldl Particles

Low density lipoproteins (LDL) are the major cholesterol carrying particles in the blood. Using cultured cells, it has been shown that LDL particles interact with specific surface receptors and are internalized via a coated pit-coated vesicle pathway for lysosomal catabolism. This (Pathway has been visualized using LDL labeled to ferritin or colloidal gold. It is now recognized that certain lysomotropic agents, such as chloroquine, inhibit lysosomal enzymes that degrade protein and cholesterol esters. By interrupting cholesterol ester hydrolysis, chloroquine treatment results in lysosomal accumulation of cholesterol esters from internalized LDL. Using LDL conjugated to colloidal gold, we have examined the ultrastructural effects of chloroquine on lipoprotein uptake by normal cultured fibroblasts.

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