Solid state fermentation of Bacillus gottheilii M2S2 in laboratory-scale packed bed reactor for tannase production

2018 ◽  
Vol 48 (9) ◽  
pp. 799-807 ◽  
Author(s):  
Subbalaxmi Selvaraj ◽  
Ramachandra Murty Vytla
2007 ◽  
Vol 145 (1-3) ◽  
pp. 87-98 ◽  
Author(s):  
Antoine A. Assamoi ◽  
Jacqueline Destain ◽  
Frank Delvigne ◽  
Georges Lognay ◽  
Philippe Thonart

2017 ◽  
Vol 40 (7) ◽  
pp. 1123-1132 ◽  
Author(s):  
Felisbela Oliveira ◽  
José Manuel Salgado ◽  
Luís Abrunhosa ◽  
Noelia Pérez-Rodríguez ◽  
José M. Domínguez ◽  
...  

2013 ◽  
Vol 96 (1) ◽  
pp. 196-199 ◽  
Author(s):  
M. Dilipkumar ◽  
N. Rajamohan ◽  
M. Rajasimman

Author(s):  
Antoine A. Assamoi ◽  
Jacqueline Destain ◽  
Frank Delvigne ◽  
Georges Lognay ◽  
Philippe Thonart

2021 ◽  
Author(s):  
Antonio Martínez-Ruiz ◽  
Luz Tovar-Castro ◽  
Cristóbal N. Aguilar ◽  
Gerardo Saucedo-Castañeda ◽  
Ernesto Favela-Torres

Abstract Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF), and fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with varied temperature, sucrose concentration and feed flow rate. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70ºC for at least 70 h of continuous operation. The highest hydrolysis rate (12.3 mg/U h) was obtained at 70ºC with 2M sucrose under batch conditions. Continuous hydrolysis in 20-mL and 200-mL bioreactors at 60ºC led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification and immobilisation stages offers an economical alternative for the development of accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.


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