Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-immobilise Aspergillus niger Biocatalyst Obtained by Solid-State Fermentation

2021 ◽  
Author(s):  
Antonio Martínez-Ruiz ◽  
Luz Tovar-Castro ◽  
Cristóbal N. Aguilar ◽  
Gerardo Saucedo-Castañeda ◽  
Ernesto Favela-Torres
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Sucrose Hydrolysis

scholarly journals Sucrose Hydrolysis in a Continuous Packed-Bed Reactor with Auto-Immobilised Aspergillus Niger Biocatalyst Obtained by Solid-State Fermentation

2021 ◽  
Author(s):  
Antonio Martínez-Ruiz ◽  
Luz Tovar-Castro ◽  
Cristóbal N. Aguilar ◽  
Gerardo Saucedo-Castañeda ◽  
Ernesto Favela-Torres
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Sucrose Concentration ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Hydrolysis Rate ◽  
Residence Times ◽  
Sucrose Hydrolysis ◽  
Operational Conditions

Abstract Invertase from Aspergillus niger C28B25 was produced by solid-state fermentation (SSF), and fermented solids were used directly as a biocatalyst for batch and continuous hydrolysis of sucrose in a packed-bed reactor under different operational conditions with varied temperature, sucrose concentration and feed flow rate. The SSF allowed obtaining a biocatalyst with an invertase activity of 82.2 U/g db. The biocatalyst maintained its activity in the range of 40 to 70ºC for at least 70 h of continuous operation. The highest hydrolysis rate (12.3 mg/U h) was obtained at 70ºC with 2M sucrose under batch conditions. Continuous hydrolysis in 20-mL and 200-mL bioreactors at 60ºC led to sucrose hydrolysis above 60% (8.5 residence times) and above 55% (4.5 residence times), respectively. The auto-immobilised biocatalyst produced by SSF without recovery, purification and immobilisation stages offers an economical alternative for the development of accessible biocatalysts that can be applied in batch or continuous sucrose hydrolysis processes. This study shows the potential of biocatalyst production by SSF for other enzymatic systems.

Optimization of solid-state fermentation of citrus dried peel by aspergillus niger in a packed bed column

1986 ◽  
Vol 6 (3) ◽  
pp. 253-258 ◽  
Author(s):  
J. A. Rodriguez ◽  
W. Bechstedt ◽  
J. Echevarria ◽  
N. Sierra ◽  
G. Delgado ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Packed Bed Column

Solid-state Fermentation of Xylanase from Penicillium canescens 10–10c in a Multi-layer-packed Bed Reactor

2007 ◽  
Vol 145 (1-3) ◽  
pp. 87-98 ◽  
Author(s):  
Antoine A. Assamoi ◽  
Jacqueline Destain ◽  
Frank Delvigne ◽  
Georges Lognay ◽  
Philippe Thonart
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Packed Bed Reactor ◽  
Penicillium Canescens

scholarly journals Valorization of Banana Peel for Citric Acid Production under Solid State Fermentation with Aspergillus niger

2020 ◽  
Vol 34 (1) ◽  
pp. 49-57
Author(s):  
Teerin Chysirichote
Keyword(s):  
Citric Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Aeration Rate ◽  
Banana Peel ◽  
Initial Ph ◽  
Glass Column ◽  
Cooling Air

Valorization of banana peel (BP) through solid state fermentation (SSF) was aimed<br /> in this research. The appropriate conditions of citric acid (CA) production by SSF of<br /> Aspergillus niger were investigated. Firstly, the optimum initial pH of the BP and the<br /> aeration rate were studied by conducting SSF in a 250-mL flask and 2-L glass column,<br /> respectively. It was found that the initial pH of the BP and aeration rates affected the CA<br /> production. The results showed that the initial pH of 5.0 and 1.0 vvm aeration were appropriate for the CA production of A. niger using BP as a substrate. The problem of rising temperature during SSF was found when applying the optimum condition to the SSF<br /> in the 20-L packed bed bioreactor (PBB), which caused a decrease in the CA production<br /> compared to that of the glass column. The cooling air jacket constructed to the PBB to<br /> remove the heat during the SSF helped increase the CA production from that in the PBB.<br /> The maximum CA production in the 20-L air-jacketed PBB was 124.0±19.2 mg g–1DS.

Intermittent agitation contributes to uniformity across the bed during pectinase production by Aspergillus niger grown in solid-state fermentation in a pilot-scale packed-bed bioreactor

2017 ◽  
Vol 121 ◽  
pp. 1-12 ◽  
Author(s):  
Anelize Terezinha Jung Finkler ◽  
Alessandra Biz ◽  
Luana Oliveira Pitol ◽  
Bruna Schweitzer Medina ◽  
Henrique Luithardt ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Pilot Scale ◽  
Packed Bed Bioreactor ◽  
Pectinase Production

Inulinase production in a packed bed reactor by solid state fermentation

2013 ◽  
Vol 96 (1) ◽  
pp. 196-199 ◽  
Author(s):  
M. Dilipkumar ◽  
N. Rajamohan ◽  
M. Rajasimman
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Packed Bed ◽  
Packed Bed Reactor
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