Effects of sucrose and pH levels on in vitro shoot regeneration from leaf explants of Bacopa monnieri and accumulation of bacoside A in regenerated shoots

2009 ◽  
Vol 100 (2) ◽  
pp. 235-239 ◽  
Author(s):  
Poornananda Madhava Naik ◽  
Shirugumbi Hanamanthagouda Manohar ◽  
Nagella Praveen ◽  
Hosakatte Niranjana Murthy
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Bacopa Monnieri ◽  
Bacoside A ◽  
In Vitro Shoot Regeneration

scholarly journals In vitro shoot regeneration of boldo from leaf explants

2010 ◽  
Vol 40 (10) ◽  
pp. 2210-2213
Author(s):  
Monalize Salete Mota ◽  
Juliana de Magalhães Bandeira ◽  
Eugenia Jacira Bolacel Braga ◽  
Valmor João Bianchi ◽  
José Antonio Peters
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Shoot Development ◽  
High Potential ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Bud Induction ◽  
Molecular Studies ◽  
In Vitro Shoot Regeneration

A shoot regeneration system for Plectranthus neochilus was studied from leaf explants. Leaves developed under in vitro conditions were cultured on Wood Plant Medium supplemented with 0.2mg dm-3 α-naphthaleneacetic acid (NAA) and different 6-benzilaminopurine (BAP) or thidiazuron (TDZ) concentrations (0, 1.5, 3.0, 4.5 and 6.0mg dm-3). An increase in percentage of responsive explants (85.3%) and in the number of shoots developed per explant (3.2) was observed when the explants were treated with 5.3 and 4.7mg dm-3 BAP, respectively. The leaf explants cultured on media supplemented with TDZ became vitreous and did not form buds. The regeneration system used is efficient for boldo bud induction and shoot development, showing high potential for advanced cellular and molecular studies.

scholarly journals In Vitro Shoot Regeneration and Polyploid Induction from Leaves of Hypericum Species

HortScience ◽  
2009 ◽  
Vol 44 (7) ◽  
pp. 1957-1961 ◽  
Author(s):  
Elisabeth M. Meyer ◽  
Darren H. Touchell ◽  
Thomas G. Ranney
Keyword(s):  
Shoot Regeneration ◽  
Chromosome Doubling ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Induction ◽  
Bluish Green ◽  
Spontaneous Chromosome ◽  
Ornamental Traits ◽  
In Vitro Shoot Regeneration

Hypericum L. H2003-004-016 is a complex hybrid among Hypericum frondosum Michx., Hypericum galioides Lam., and Hypericum kalmianum L. and exhibits valuable ornamental characteristics, including compact habit, bluish green foliage, and showy flowers. Inducing polyploidy may further enhance the ornamental traits of this hybrid and provide new opportunities for hybridizing with other naturally occurring polyploid Hypericum sp. In this study, in vitro shoot regeneration and treatment of regenerative callus with the dinitroaniline herbicide oryzalin (3,5-dinitro-N4,N4-dipropylsufanilamide) were investigated as a means of inducing allopolyploidy. First, in vitro regeneration was optimized for callus and shoot induction by culture of leaf explants on medium supplemented with benzylamino purine (BA) or meta-topolin (mT) at 5, 10, or 15 μM in combination with indoleacetic acid (IAA) at 0, 1.25, 2.5, or 5 μM. Both BA and mT treatments successfully induced regenerative callus and shoots. Multiple regression analysis estimated maximum regenerative callus (94%) and shoot induction (18 shoots per explant) in medium supplemented with 5 μM BA and 3.75 μM IAA. In the second part of the study, exposure of regenerative callus to oryzalin at 0, 7.5, 15, 30, 60, or 90 μM for durations of 3, 6, or 9 d was investigated for polyploid induction. There was no survival for any of the calli in the 60- or 90-μM oryzalin treatments, but calli subjected to the other treatments exhibited some survival and polyploid induction. Duration had no effect on callus survival or ploidy level, but oryzalin concentration was a significant factor in both. The greatest percentage (44%) of polyploids was induced with 30 μM oryzalin. Spontaneous chromosome doubling was observed in 8% of control explants receiving no oryzalin treatment.

The Effect of Heavy Metals on In Vitro Adventitious Shoot Production and Bacoside A Content in Bacopa Monnieri (L)

2017 ◽  
Vol 14 (4) ◽  
pp. 1-10
Author(s):  
Poornananda Madhava Naik Manoj Godbole
Keyword(s):  
Heavy Metals ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Dry Weight ◽  
Bacopa Monnieri ◽  
Optimum Number ◽  
Bacoside A ◽  
Shoot Production

In the present study the effect of heavy metals (MnSO 4, ZnSO 4 and CuSO 4 ) on adventitious shoot regeneration, biomass and bacoside A accumulation in Bacopa monnieri was carried out. The leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg l -1 kinetin (Kin) with varying concentration of heavy metals (Control: Mn-0.10 mM, Zn-0.03 mM and Cu- 0.0001mM; Mn: 0.20, 0.40, 0.80 and 1.60 mM; Zn: 0.06, 0.12, 0.24 and 0.48 mM; Cu: 0.02, 0.05, 0.10 and 0.20 mM). Optimum number of adventitious shoots (123.50 shoot/explants), fresh weight (3.826 g) and dry weight (0.226 g) of Bacopa monnieri were obtained in the medium with 0.12 mM Zn concentration. The highest production

Enhancement of in vitro shoot regeneration from leaf explants of apple rootstock G.41

2013 ◽  
Vol 50 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Xin Zhang ◽  
Yuan Qin ◽  
Dong Liang ◽  
Yangjun Zou ◽  
Fengwang Ma
Keyword(s):  
Shoot Regeneration ◽  
Leaf Explants ◽  
Apple Rootstock ◽  
In Vitro Shoot Regeneration

scholarly journals Influence of light-emitting diodes and benzylaminopurin on adventitious shoot regeneration of water hyssop (Bacopa monnieri (L.) pennell) in vitro

2016 ◽  
Vol 68 (3) ◽  
pp. 501-508 ◽  
Author(s):  
Mehmet Karataş ◽  
Muhammad Aasim ◽  
Muraz Dazkirli
Keyword(s):  
Shoot Regeneration ◽  
Light Emitting Diodes ◽  
Tap Water ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Bacopa Monnieri ◽  
Lower Half ◽  
Light Emitting ◽  
Number Of Shoots

Water hyssop (Bacopa monnieri (L.) Pennell) is a medicinal plants. Its upper and lower halves of leaf explants were incubated in Murashige and Skoog (MS) medium supplemented with 0.25, 0.50 and 1.0 mg/L benzylaminopurine (BA) for 8 weeks; the explants were exposed to white (W) and red and blue (R and B, respectively) light-emitting diodes (LEDs), at 4:1, 3:1, 2:1 and 1:1 R and B light ratios, respectively. Shoot regeneration (100%) was achieved from all explants at all applied concentrations of BA and LED types. All explants showed different BA concentration requirements for regeneration of the maximum number of shoots. Longer shoots were obtained on medium with 0.25 mg/L BA. The W LED lighting system was found to be more effective for regenerating the maximum number of shoots (26.11) per explant (on the upper half of the leaf). Conversely, longer and shorter shoots were generated under 1:1 R:B and W LEDs, respectively. The number of shoots per explant ranged from 9.67-24.0 (full leaf), 6.33-25.92 (lower half of the leaf) and 7.33-27.33 (upper half of the leaf), respectively, in response to BA and LED light. Shoot length ranged from 0.94-1.90 cm (full lamina), 0.70-2.11 cm (lower half of the leaf) and 0.93-1.83 cm (upper half of the lamina) in response to BA and LED lifght. Regenerated shoots were successfully rooted using indole-3-butyric acid (IBA) and acclimatized in the aquarium provided with tap water.

In Vitro Shoot Regeneration from Callus Derived from Marubakaido Apple Rootstock under Different Aluminium Concentrations

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 460e-460 ◽  
Author(s):  
Marisa F. de Oliveira ◽  
Gerson R. de L. Fortes ◽  
João B. da Silva
Keyword(s):  
Shoot Regeneration ◽  
Dichlorophenoxyacetic Acid ◽  
Apple Rootstock ◽  
Under Five ◽  
Significant Difference ◽  
Previously Treated ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
In Vitro Shoot Regeneration

The aim of this work was to evaluate the organogenesis of Marubakaido apple rootstock under different aluminium concentratons. The explants were calli derived from apple internodes treated with either 2,4-dichlorophenoxyacetic acid or pichloram at 0.5 and 1.0 μM and under five different aluminium concentrations (0, 5, 10, 15, 20 mg/L). These calli were then treated with aluminium at 0, 5, 10, 15, and 20 mg/L. It was observed shoot regeneration only for those calli previously treated with pichloram. There were no significant difference among the aluminium concentrations.

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

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