Molecular cloning and characterization of the genic male sterility related geneCYP86MFin Chinese cabbage (Brassica campestrisL. ssp. chinensis Makino var.communisTsen et Lee)

2003 ◽  
Vol 78 (3) ◽  
pp. 319-323 ◽  
Author(s):  
Wan-Zhi Ye ◽  
Jia-Shu Cao ◽  
Xun Xiang ◽  
Guang-Wen Zeng
2009 ◽  
Vol 134 (6) ◽  
pp. 632-640 ◽  
Author(s):  
Li Huang ◽  
Wan-zhi Ye ◽  
Ting-ting Liu ◽  
Jia-shu Cao

Cytological features of ‘Aijiaohuang’ chinese cabbage-pak-choi (Brassica campestris ssp. chinensis) Bcajh97-01A/B genic male-sterile AB line were examined to determine phenotypic reasons for male sterility. The sterile line Bcajh97-01A was found to undergo aberrant cytokinesis during male meiosis. Transcriptional profiling of the flower buds of both fertile and sterile plants was performed at the periods preceding meiosis, at the tetrad to uninucleate pollen period, and at the binucleate to mature pollen period. Transcript-derived fragments (TDFs) from corresponding genes that were expressed in flower buds at these three different stages could be divided into nine classes. We sequenced a total of 14 new TDFs that were differentially displayed at particular pollen developmental stages, including eight genes with unknown or hypothetical functions and six genes showing significant homology with known genes. This characterization of the Bcajh97-01A genic male-sterile line allowed the identification of candidate genes underlying genic male sterility.


1998 ◽  
pp. 133-138
Author(s):  
F. Hui ◽  
W. Yutang ◽  
J. Shujuan ◽  
J. Gang ◽  
J. Jusheng ◽  
...  

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