Simultaneous determination of ochratoxin A and enterotoxin A in milk by magnetic nanoparticles based fluorescent immunoassay

Doenjang, a Korean fermented soybean paste, is vulnerable to contamination by mycotoxins because it is directly exposed to environmental microbiota during fermentation. A method that simultaneously determines 20 mycotoxins in doenjang, including aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEN), and fumonisins (FBs) with an immunoaffinity column cleanup was optimized and validated in doenjang using LC-MS/MS. The method showed good performance in the analysis of 20 mycotoxins in doenjang with good linearity (R2 > 0.999), intra- and inter-day precision (<16%), recovery (72–112%), matrix effect (87–104%), and measurement uncertainty (<42%). The validated method was applied to investigate mycotoxin contamination levels in commercial and homemade doenjang. The mycotoxins that frequently contaminated doenjang were AFs, OTA, ZEN, and FBs and the average contamination level and number of co-occurring mycotoxins in homemade doenjang were higher than those in commercially produced doenjang.

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Simultaneous determination of aflatoxins and ochratoxin A in baby foods and paprika by HPLC with fluorescence detection: A single-laboratory validation study

Talanta ◽

10.1016/j.talanta.2010.11.031 ◽

2011 ◽

Vol 83 (5) ◽

pp. 1442-1446 ◽

Cited By ~ 29

Author(s):

C. Brera ◽

F. Debegnach ◽

B. De Santis ◽

E. Pannunzi ◽

C. Berdini ◽

...

Keyword(s):

Simultaneous Determination ◽

Ochratoxin A ◽

Fluorescence Detection ◽

Validation Study ◽

Baby Foods ◽

Single Laboratory

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Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

Journal of AOAC International ◽

10.5740/jaoacint.14-211 ◽

2015 ◽

Vol 98 (4) ◽

pp. 939-945 ◽

Cited By ~ 4

Author(s):

Işil Gazioğlu ◽

Ufuk Kolak

Keyword(s):

Quantitative Analysis ◽

Simultaneous Determination ◽

Ochratoxin A ◽

Fluorescence Detection ◽

Aflatoxin B1 ◽

Hplc Analysis ◽

Extraction Procedure ◽

Immunoaffinity Column ◽

Rp Hplc

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

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