ALLOGRAFT REJECTION IN C'6 DEFECTIVE RABBITS

27 skin allografts were transplanted in duplicate to 13 rabbits defective in the sixth component (C'6) of complement (C'). 14 were rejected within the normal period of time and 9 only after a significant delay. In four grafts, no rejection was observed. Grafts exchanged between C' active and C' defective littermates tended to persist viable longer on the C' defective partners. It is concluded that in a species of higher vertebrates, the rabbit, allograft rejection can proceed in the absence of C'6 and the biological functions depending on it. This includes the cytotoxic function of complement. However, the prolonged survival in C'6 defective rabbits of some allografts strongly suggests that two pathways may be operative in the rejection reaction. The results are consistent with the view that a mechanism independent of C'6 was alone fully effective in the majority of the donor-host combinations. A C'6-dependent mechanism became influential only in some specific donor-host combinations.

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The Allograft Rejection Reaction: The Leading Cause of Late Failure of Clinical Corneal Grafts

Ciba Foundation Symposium 15 - Corneal Graft Failure - Novartis Foundation Symposia ◽

10.1002/9780470719985.ch9 ◽

2008 ◽

pp. 151-167 ◽

Cited By ~ 15

Author(s):

Ali A. Khodadoust

Keyword(s):

Allograft Rejection ◽

Rejection Reaction ◽

Late Failure

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Prolonged Survival of Skin Allografts after Treatment with Direct Current

European Surgical Research ◽

10.1159/000127460 ◽

1969 ◽

Vol 1 (1) ◽

pp. 50-55 ◽

Cited By ~ 2

Author(s):

G. Lemperle ◽

H.E. Köhnlein ◽

R. Mende

Keyword(s):

Direct Current ◽

Prolonged Survival ◽

Skin Allografts

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PROLONGED SURVIVAL OF MOUSE SKIN ALLOGRAFTS IN RECIPIENTS TREATED WITH DONOR SPLENOCYTES AND ANTIBODY TO CD40 LIGAND1

Transplantation Journal ◽

10.1097/00007890-199707270-00026 ◽

1997 ◽

Vol 64 (2) ◽

pp. 329-335 ◽

Cited By ~ 127

Author(s):

Thomas G. Markees ◽

Nancy E. Phillips ◽

Randolph J. Noelle ◽

Leonard D. Shultz ◽

John P. Mordes ◽

...

Keyword(s):

Mouse Skin ◽

Prolonged Survival ◽

Skin Allografts

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THE SENSITIZATION OF RATS BY ALLOGRAFTS TRANSPLANTED TO ALYMPHATIC PEDICLES OF SKIN

Journal of Experimental Medicine ◽

10.1084/jem.133.5.951 ◽

1971 ◽

Vol 133 (5) ◽

pp. 951-962 ◽

Cited By ~ 42

Author(s):

N. L. Tilney ◽

J. L. Gowans

Keyword(s):

Life Span ◽

Lymphatic Drainage ◽

Prolonged Survival ◽

Peripheral Sensitization ◽

Skin Allografts ◽

Rapid Destruction ◽

Immunological Attack

Pedicles of skin which lacked a lymphatic drainage were raised on the backs of rats in order to study the importance of afferent lymphatics in sensitization by skin allografts. Although allografts transplanted to the alymphatic pedicles enjoyed a prolonged survival, they contracted progressively from about 3 wk after transplantation and were reduced eventually to small scars. In contrast, autografts survived unchanged in size for the life-span of the pedicles which carried them. The slow contracture of the allografts was associated with sensitization of the host because test allografts applied orthotopically were destroyed with a second-set tempo. No regeneration of lymphatics from the long-standing pedicles could be demonstrated, and it was concluded that sensitization had occurred eventually through the blood, presumably by the process of peripheral sensitization. Allografts on skin pedicles could be destroyed rapidly by active or adoptive immunization, so it is probable that the level of sensitization to which they themselves gave rise was a low one. Although it is not disputed that afferent lymphatics are essential for the rapid destruction of skin allografts, it is clear that the absence of a lymphatic supply does not permanently exempt them from immunological attack in the rat.

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Prolonged survival of skin allografts exposed to heterologous antilymphocyte serum in vitro

Journal of Surgical Research ◽

10.1016/0022-4804(69)90075-4 ◽

1969 ◽

Vol 9 (6) ◽

pp. 327-330 ◽

Cited By ~ 8

Author(s):

S. Raju ◽

James B. Grogan ◽

James D. Hardy

Keyword(s):

Prolonged Survival ◽

Antilymphocyte Serum ◽

Skin Allografts

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Virus-Induced Abrogation of Transplantation Tolerance Induced by Donor-Specific Transfusion and Anti-CD154 Antibody

Journal of Virology ◽

10.1128/jvi.74.5.2210-2218.2000 ◽

2000 ◽

Vol 74 (5) ◽

pp. 2210-2218 ◽

Cited By ~ 120

Author(s):

Raymond M. Welsh ◽

Thomas G. Markees ◽

Bruce A. Woda ◽

Keith A. Daniels ◽

Michael A. Brehm ◽

...

Keyword(s):

Allograft Rejection ◽

Viral Infections ◽

Acute Infection ◽

Tolerance Induction ◽

Lymphocytic Choriomeningitis ◽

Murine Cytomegalovirus ◽

Transplantation Tolerance ◽

High Dose ◽

Pichinde Virus ◽

Skin Allografts

ABSTRACT Treatment with a 2-week course of anti-CD154 antibody and a single transfusion of donor leukocytes (a donor-specific transfusion or DST) permits skin allografts to survive for >100 days in thymectomized mice. As clinical trials of this methodology in humans are contemplated, concern has been expressed that viral infection of graft recipients may disrupt tolerance to the allograft. We report that acute infection with lymphocytic choriomeningitis virus (LCMV) induced allograft rejection in mice treated with DST and anti-CD154 antibody if inoculated shortly after transplantation. Isografts resisted LCMV-induced rejection, and the interferon-inducing agent polyinosinic:polycytidylic acid did not induce allograft rejection, suggesting that the effect of LCMV is not simply a consequence of nonspecific inflammation. Administration of anti-CD8 antibody to engrafted mice delayed LCMV-induced allograft rejection. Pichinde virus also induced acute allograft rejection, but murine cytomegalovirus and vaccinia virus (VV) did not. Injection of LCMV ∼50 days after tolerance induction and transplantation had minimal effect on subsequent allograft survival. Treatment with DST and anti-CD154 antibody did not interfere with clearance of LCMV, but a normally nonlethal high dose of VV during tolerance induction and transplantation killed graft recipients. We conclude that DST and anti-CD154 antibody induce a tolerant state that can be broken shortly after transplantation by certain viral infections. Clinical application of transplantation tolerance protocols may require patient isolation to facilitate the procedure and to protect recipients.

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