scholarly journals INDUCTION OF ARGINASE ACTIVITY WITH THE SHOPE PAPILLOMA VIRUS IN TISSUE CULTURE CELLS FROM AN ARGININEMIC PATIENT

1973 ◽  
Vol 137 (4) ◽  
pp. 1091-1096 ◽  
Author(s):  
Stanfield Rogers ◽  
A. Lowenthal ◽  
H. G. Terheggen ◽  
J. P. Columbo
Keyword(s):  
Tissue Culture ◽  
Human Fibroblasts ◽  
Arginase Activity ◽  
Tissue Cultures ◽  
Papilloma Virus ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Shope Papilloma Virus

Inoculation of the Shope virus in tissue cultures of human fibroblasts from a patient with a deficiency of the enzyme arginase results in an induction of arginase activity, apparently virus coded.

Transformation of Bovine Tissue Culture Cells by Bovine Papilloma Virus

Nature ◽  
1963 ◽  
Vol 199 (4897) ◽  
pp. 1016-1018 ◽  
Author(s):  
PAUL H. BLACK ◽  
JANET W. HARTLEY ◽  
WALLACE P. ROWE ◽  
ROBERT J. HUEBNER
Keyword(s):  
Tissue Culture ◽  
Papilloma Virus ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Bovine Tissue ◽  
Bovine Papilloma Virus

Further studies on tumor-specific immunity induced by nontumorigenic mouse ascites tissue-culture cells

1972 ◽  
Vol 18 (6) ◽  
pp. 775-781 ◽  
Author(s):  
C. P. Eng ◽  
J. F. Morgan
Keyword(s):  
Tissue Culture ◽  
Solid Tumor ◽  
Tumor Development ◽  
Tissue Cultures ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Mouse Ascites ◽  
Physical Treatments ◽  
High Degree

Long-term tissue cultures derived from the TA3 ascites tumor of the mouse became nontransplantable but induced a high degree of immunoprotection against challenge with as many as 106 virulent cells. Intraperitoneal immunization proved more effective than the intravenous or intramuscular routes. After chemical and physical treatments virulent TA3 cells showed no immunoprotective capacity. After similar treatments the nontumorigenic TA3 tissue-culture cells showed a moderate immunoprotective capacity. Nontumorigenic cells of the 6C3HED and TA3 lines induced a high degree of immunoprotection against solid tumor development produced by the subcutaneous injection of as many as 107 virulent cells. Protection against solid tumor development could be demonstrated provided immunization with nontumorigenic cells was begun within 2 weeks of implantation of the virulent cells.

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

1993 ◽  
Vol 51 ◽  
pp. 342-343
Author(s):  
Heide Schatten ◽  
Neidhard Paweletz ◽  
Ron Balczon
Keyword(s):  
Tissue Culture ◽  
Cell Cycle Progression ◽  
Group Formation ◽  
Sulfhydryl Group ◽  
Mammalian Tissue ◽  
Mitotic Chromosomes ◽  
Microtubule Network ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

UDP-D-glucose 4-epimerase activity of the tissue culture of white poplars (Populus alba L. var. pyramidalis)

1982 ◽  
Vol 47 (5) ◽  
pp. 1530-1536 ◽  
Author(s):  
Ladislav Bilisics ◽  
Štefan Karácsonyi ◽  
Marta Kubačková
Keyword(s):  
Tissue Culture ◽  
Cell Walls ◽  
Enzyme Preparation ◽  
Uridine Diphosphate ◽  
Populus Alba ◽  
Activity Change ◽  
White Poplar ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Galactose Content

The presence of UDP-D-glucose 4-epimerase (EC 5.1.3.2) in the culture tissue of white poplar was evidenced. As found, the partially purified enzyme preparation contained UDP-D-glucose glucosyltransferase, UDP-D-galactose galactosyltransferase and non-specific enzymes able to cleave the uridine-diphosphate saccharides into the appropriate hexose monophosphates. The activity change of UDP-D-glucose 4-epimerase in tissue culture cells during the growth was in accord with changes in D-galactose content in cell walls and indicated the possibility to regulate the formation of polysaccharides containing D-galactose at the level of production of UDP-D-galactose in cells.

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