scholarly journals GENETICS OF A NEW IgVH (T15 IDIOTYPE) MARKER IN THE MOUSE REGULATING NATURAL ANTIBODY TO PHOSPHORYLCHOLINE

1974 ◽  
Vol 139 (4) ◽  
pp. 983-1001 ◽  
Author(s):  
R. Lieberman ◽  
M. Potter ◽  
E. B. Mushinski ◽  
W. Humphrey ◽  
S. Rudikoff
Keyword(s):  
Recombinant Inbred ◽  
Inbred Strains ◽  
Normal Serum ◽  
Natural Antibody ◽  
Myeloma Protein ◽  
Germ Free ◽  
Inbred Strains Of Mice ◽  
Low Levels ◽  
Linkage Of Genes ◽  
F2 Progeny

The idiotype present on the Fab of a phosphorylcholine-binding IgA myeloma protein TEPC 15 (T15) of BALB/c origin was found in normal serum of BALB/c mice. Molecules carrying the T15 idiotype in normal serum could be adsorbed with Sepharose phosphorylcholine beads and R36A pneumococci. The T15 idiotype is absent in germ-free BALB/c but appears when the mice are conventionalized. A survey of normal sera of inbred strains for the T15 idiotype showed it to be present in BALB/c, 129, C57L, C58, and ST and absent or in low levels in CBA, C3H, C57BL/6, C57BL/Ka, C57BL/10, SJL, B10.D2, DBA/2, RIII, A, AL, AKR, NZB, and NH inbred strains of mice. The T15 idiotype is associated with some but not all strains carrying the IgCH allotypes found in BALB/c. Linkage of genes controlling the T15 idiotype in normal serum to the IgCH locus of BALB/c was demonstrated in F2 progeny of a BALB/c and C57BL cross, Bailey's recombinant inbred strains, C x BD, C x BE, C x BG, C x BH, C x BI, C x BJ, C x BK, and CB20 congenic strains. Among these strains, only those possessing the IgCH locus of BALB/c including the F2 progeny consisting of BALB/c homozygotes and BALB/c/C57BL heterozygotes and C x BG and C x BJ recombinants showed the T15 idiotype.

GENETIC ANALYSIS OF PLASMA CORTICOSTERONE LEVELS IN TWO INBRED STRAINS OF MICE

1972 ◽  
Vol 55 (2) ◽  
pp. 415-420 ◽  
Author(s):  
B. E. ELEFTHERIOU ◽  
D. W. BAILEY
Keyword(s):  
Genetic Analysis ◽  
Recombinant Inbred ◽  
Epistatic Interaction ◽  
Genetic Model ◽  
Inbred Strains ◽  
Plasma Corticosterone ◽  
Experimental Results ◽  
Recombinant Inbred Strains ◽  
Inbred Strains Of Mice

SUMMARY Plasma corticosterone levels were determined fluorometrically in mice of two unrelated highly inbred strains, C57BL/6By and BALB/cBy, and in seven of their derived recombinant-inbred strains as well as their F1 hybrid and backcross generations necessary to arrive at a genetic model for plasma corticosterone levels. It was concluded that the simplest genetic model, and one which fits the experimental results, was one which assumed that plasma corticosterone levels are controlled genetically by two loci with the epistatic interaction indicating dependency of pathways of action for the two genes.

scholarly journals Genetic Contribution to Initial and Progressive Alcohol Intake Among Recombinant Inbred Strains of Mice

2018 ◽  
Vol 9 ◽  
Author(s):  
Megan K. Mulligan ◽  
Wenyuan Zhao ◽  
Morgan Dickerson ◽  
Danny Arends ◽  
Pjotr Prins ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Alcohol Intake ◽  
Inbred Strains ◽  
Recombinant Inbred Strains ◽  
Genetic Contribution ◽  
Inbred Strains Of Mice

The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci.

Genetics ◽  
1990 ◽  
Vol 125 (2) ◽  
pp. 431-446
Author(s):  
E M Eicher ◽  
B K Lee
Keyword(s):  
X Chromosome ◽  
Mammary Tumor ◽  
Recombinant Inbred ◽  
Inbred Strains ◽  
Genetic Profile ◽  
Inbred Strains Of Mice ◽  
Strain Data ◽  
Mink Cell ◽  
Using Data ◽  
Murine Leukemia

Abstract We report the construction of 17 recombinant inbred (RI) strains of mice derived from the progenitor strains NZB/BINRe and SM/J and the typing of this RI strain set, designated NXSM, for 58 loci distributed on 16 autosomes and the X chromosome. Two backcrosses involving NZB/BINJ and SM/J were constructed to confirm chromosomal assignments and determine gene orders suggested from NXSM RI strain data. From these results we recommend that chromosomal assignments and gene orders suggested from analyses of RI strain sets be confirmed using data obtained by other means. We also typed NZB/BINJ and SM/J for mammary tumor proviral (Mtv) loci. Both strains share three previously described Mtv loci: Mtv-7, Mtv-14 and Mtv-17. In addition, NZB/BINJ contains the previously described Mtv-3 and Mtv-9 loci and two new Mtv proviral loci: Mtv-27 located on chromosome (Chr) 1 and Mtv-28 located on the X chromosome. SM/J contains the previously described loci Mtv-6 and Mtv-8. Four LTR, mink cell focus-forming murine leukemia viral loci were identified and mapped: Ltrm-1 on Chr 12, Ltrm-2 on Chr 16, Ltrm-3 on Chr 5, and Ltrm-4 on Chr 13. The Tgn locus was positioned proximal to the Ly-6 locus on Chr 15.

scholarly journals Genetic Analysis of Tongue Size and Taste Papillae Number and Size in Recombinant Inbred Strains of Mice

2008 ◽  
Vol 33 (8) ◽  
pp. 693-707 ◽  
Author(s):  
D. J. Reiner ◽  
T. A. Jan ◽  
J. D. Boughter ◽  
C.-X. Li ◽  
L. Lu ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Recombinant Inbred ◽  
Inbred Strains ◽  
Recombinant Inbred Strains ◽  
Inbred Strains Of Mice

scholarly journals Susceptibility to phenytoin-induced cleft lip with or without cleft palate: many genes are involved

1987 ◽  
Vol 49 (1) ◽  
pp. 43-49 ◽  
Author(s):  
I. Jill Karolyi ◽  
Sharon Liu ◽  
Robert P. Erickson
Keyword(s):  
Glucocorticoid Receptor ◽  
Cleft Palate ◽  
Recombinant Inbred ◽  
Cleft Lip ◽  
Inbred Strains ◽  
Cyclic Monophosphate ◽  
Major Histocompatibility Locus ◽  
Inbred Strains Of Mice ◽  
Histocompatibility Locus ◽  
Isolated Cleft Palate

SummaryIn a search for genetic differences in susceptibility to cleft lip with or without cleft palate [CL(P)], congenic and recombinant inbred strains of mice were treated with phenytoin or control injections. Of six loci tested, five were found to affect susceptibility to phenytoin-induced and/or sporadic CL(P): (1) the major histocompatibility locus, H-2; (2) the locus controlling β2-microglobulin, B2m; (3) a locus controlling β-glucuronidase, Gus; (4) the locus controlling N-acetyl transferase, Nat; and (5) the locus for brown pigmentation, b. B2m and Gus only affected the sporadic incidence of CL(P), while the b locus only affected phenytoin-induced incidence of CL(P). Three of these loci are also known to affect glucocorticoid-induced isolated cleft palate (CP), but different alleles of the loci are involved. Phenytoin did not affect levels of adenosine 3′,5′-cyclic monophosphate (cAMP) in palates and tongues of day 15 fetuses. A comparison of glucocorticoid receptor parameters with the incidence of phenytoin-induced CL(P) found no correlation.

scholarly journals Differential expression of an equivalent clonotype among BALB/c and C57BL/6 mice

1978 ◽  
Vol 147 (1) ◽  
pp. 1-12 ◽  
Author(s):  
MP Cancro ◽  
NH Sigal ◽  
NR Klinman
Keyword(s):  
Serum Levels ◽  
Inbred Strains ◽  
Cross Reactivity ◽  
Normal Serum ◽  
Cell Populations ◽  
Myeloma Protein ◽  
Regulatory Processes ◽  
Histocompatibility Complex ◽  
Immunoglobulin Allotype

The primary anti-phosphorylcholine (PC) response in BALB/c, C57BL/6, and congenic and recombinant inbred strains of these parental types has been examined in the splenic focus system. The frequencies of PC-specific precursors were shown to vary among these strains from 2 to 20 precursors per 10(6) splenic B cells. The distribution of these frequencies suggests that elements closely linked to or within the major histocompatibility complex may play a role in the determination of this parameter, although additional experiments are necessary to adequately assess this possibility. Moreover, all strains tested, regardless of immunoglobulin allotype, expressed monoclonal antibodies indistinguishable from the TEPC 15 myeloma protein (T15) clonotype. Further, the frequency of this clonotype in a given strain did not appear related to allotype, since both high and low T15 frequencies were found among strains of either the BALB/c (a(1)) or C57BL/6 (a(2)) allotype. The examination of normal serum for the T15 idiotype, however, revealed that only mice of the BALB/c allotype (a(1)) expressed the T15 idiotype in detectable quantities. After immunization with Diplococcus pneumoniae, sera from mice of the a(1) allotype consistently contained large quantities of the T15 idiotype, whereas sera from mice of the a(2) allotype exhibited various degrees of cross-reactivity with anti-T15 antibody. These results suggest that: (a) the allotype of an individual, although closely related to serum levels of an idiotype, is unrelated to the proportion of the precursor population which expresses that idiotype and; (b) the serum expression of a given idiotype may reflect regulatory processes, which act either during or before antigenic stimulation, rather than the actual clonotype representation in the repertoire. These findings indicate that distinctions must be made between the expression of idiotypic determinants within precursor B-cell populations and elements which regulate the subsequent appearance of those idiotypes in serum antibodies.

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