scholarly journals Immunochemical characterization of the "native" type III polysaccharide of group B Streptococcus.

1976 ◽  
Vol 143 (2) ◽  
pp. 258-270 ◽  
Author(s):  
C J Baker ◽  
D L Kasper ◽  
C E Davis
Keyword(s):  
Group B Streptococcus ◽  
Molecular Size ◽  
Antigenic Determinants ◽  
Capsular Type ◽  
Polysaccharide Antigen ◽  
Native Form ◽  
Intact Cells ◽  
Type Iii ◽  
Group B

The type III polysaccharide of -roup B Streptococcus has been isolated and purified by a method that employs washing of intact cells at neutral pH. That the polysaccharide prepared by this procedure is the "native" type III antigen is suggested by its molecular size in excess of 10(6) daltons, its degradation by acid and heat treatment to a fragment with immunologic characteristics of the classical HCl antigen, and its type-specific serologic activity. The type III polysaccharide in native form contains sialic acid, galactose, glucose, glucosamine, heptose, and mannose. It is acidic in nature, is resistant to neuramindiase degradation, contains no O-acetyl groups, and does not share antigenic determinants with capsular type K1 antigen of Escherichia coli or Group B polysaccharide antigen of Neiserria meningitidis.

Group B Streptococcal and Pneumococcal Sepsis in Newborn Infants: The Role of Pneumococcal Antibody

PEDIATRICS ◽  
1978 ◽  
Vol 62 (4) ◽  
pp. 620-621
Author(s):  
Gerald W. Fischer ◽  
James W. Bass ◽  
George H. Lowell ◽  
Martin H. Crumrine
Keyword(s):  
Streptococcus Pneumoniae ◽  
Hydrochloric Acid ◽  
Group B Streptococcus ◽  
Newborn Infants ◽  
Polysaccharide Antigen ◽  
Type Iii ◽  
Pneumococcal Sepsis ◽  
Group B

The article by Bortolussi et al. on pneumococcal septicemia and meningitis in the neonat (Pediatrics 60:352, September 1977) was of great interest to us, since we have been analyzing the effect of antibody directed against Streptococcus pneumoniae on group B Streptococcus type III. We have recently shown (unpublished data) that antibody directed against S. pneumoniae type 14 precipitates the hot hydrochloric acid-extracted polysaccharide antigen of group B Streptococcus type III. Further studies have shown that this antibody is opsonic for group B Streptococcus type III in an in vitro bactericidal assay and protective in a suckling rat model of group B Streptococcus type III sepsis.1

Demonstration and Characterization of Buoyant-Density Subpopulations of Group B Streptococcus Type III

1990 ◽  
Vol 161 (4) ◽  
pp. 741-746 ◽  
Author(s):  
S. Hakansson ◽  
M. Granlund-Edstedt ◽  
M. Sellin ◽  
S. E. Holm
Keyword(s):  
Buoyant Density ◽  
Group B Streptococcus ◽  
Type Iii ◽  
Group B

Structural determination and serology of the native polysaccharide antigen of type-III group B Streptococcus

1980 ◽  
Vol 58 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Harold J. Jennings ◽  
Karl-Gunnar Rosell ◽  
Dennis L. Kasper
Keyword(s):  
Sialic Acid ◽  
Group B Streptococcus ◽  
Rabbit Antiserum ◽  
Ph Control ◽  
Molar Ratio ◽  
Polysaccharide Antigen ◽  
Type Iii ◽  
Group B ◽  
Unit Formula ◽  
Ph Controlled

The native polysaccharide antigen isolated from type-III group B Streptococcus contains D-galactose (Gal), D-glucose (Glc), 2-acetamido-2-deoxy-D-glucose (GlcNAc), and sialic acid (NeuNAc) in the molar ratio of 2:1:1:1 and its structure can be represented by the following repeating unit:[Formula: see text]By cleavage of all the labile sialic acid end groups the incomplete type-III antigen is obtained which is structurally identical to the S. pneumoniae type-14 polysaccharide. Thus, the native type-III polysaccharide is serologically distinct from the incomplete type-III antigen by virtue of the former having determinants terminating in sialic acid and the latter in β-D-galactopyranose units. None of these latter determinants could be detected in streptococcal organisms grown under pH-controlled conditions (pH 7.0) or in rabbit antiserum made to these pH-controlled organisms. However, in antisera produced in rabbits to the same organisms grown without pH control (Lancefield procedures), antibodies to both types of determinant could be detected. This can be attributed to the removal of some of the masking sialic acid residues from the cell-associated native polysaccharide by degradative procedures which occur during these latter conditions.

scholarly journals Protection of mice from experimental infection with type III group B Streptococcus using monoclonal antibodies.

1983 ◽  
Vol 158 (3) ◽  
pp. 1006-1011 ◽  
Author(s):  
M L Egan ◽  
D G Pritchard ◽  
H C Dillon ◽  
B M Gray
Keyword(s):  
Monoclonal Antibodies ◽  
Experimental Infection ◽  
Group B Streptococcus ◽  
Antigenic Determinants ◽  
Group B Streptococci ◽  
Lethal Challenge ◽  
Type Iii ◽  
Group B

Mouse hybridoma antibodies of several major classes against group B streptococcus type III have been produced. Mice were immunized with either whole heat-killed or acid-treated organisms to obtain antibodies against both the complete (sialated) or incomplete (nonsialated) forms of the type III polysaccharide. Resulting monoclonal antibodies showed exclusive specificity for either the complete or incomplete antigen. The ability of these antibodies to protect mice from a lethal challenge of live type III organisms was tested with a mucin model that permitted use of very small inocula given intraperitoneally with antibody and mucin. Antibodies specific for the nonsialated antigen were not protective, whether of IgM, IgG2a, or IgG3 isotypes. Antibodies specific for the complete antigen were, however, highly protective, including monoclonals of IgM, IgG2a, and IgA isotypes. These mouse monoclonal antibodies against group B streptococci that are directed against either complete or incomplete antigenic determinants, and include isotypes other than IgM, should be particularly useful for studying the mechanism of protection against experimental infection.

scholarly journals Characterization of Group B Streptococcus Isolated from Women in Saitama City, Japan

2012 ◽  
Vol 65 (6) ◽  
pp. 516-521 ◽  
Author(s):  
Hiroyuki Ueno ◽  
Yoshihiko Yamamoto ◽  
Akiko Yamamichi ◽  
Koji Kikuchi ◽  
Sumie Kobori ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Group B
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