scholarly journals The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. I. Proliferative response.

1977 ◽  
Vol 145 (1) ◽  
pp. 151-162 ◽  
Author(s):  
A S Kong ◽  
S I Morse
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Dna Replication ◽  
Bordetella Pertussis ◽  
Proliferative Response ◽  
Bone Marrow Cells ◽  
Specific Antigen ◽  
Lymph Node Cells ◽  
In Vitro Effects

The lymphocytosis-promoting factor of Bordetella pertussis is a potent mitogen for murine lymphocytes in vitro. The stimulatory response was not the result of specific antigen stimulation. Spleen and lymph node cells were responsive, whereas normal thymocytes were unresponsive. However, DNA replication was induced in cortisone-resistant thymocytes by lymphocytosis-promoting factor (LPF). Bone marrow cells were not stimulated by LPF.

scholarly journals Truncated mu (mu') chains in murine IgM. Evidence that mu' chains lack variable regions.

1985 ◽  
Vol 162 (6) ◽  
pp. 1862-1877 ◽  
Author(s):  
R Marks ◽  
M J Bosma
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Molecular Mass ◽  
Tumor Cells ◽  
Isoelectric Focusing ◽  
Bone Marrow Cells ◽  
Normal Serum ◽  
Antigenic Determinants ◽  
Variable Regions ◽  
Lymph Node Cells

Secreted IgM was shown to contain truncated mu (mu') chains with an apparent molecular mass of approximately 55 kD. The estimated percentage of IgM heavy (H) chains in the mu' form ranged from less than or equal to 1% in the case of one tumor IgM protein (104E) to greater than or equal to 30% in normal serum IgM. Serum mu' chains lacked antigenic determinants characteristic of immunoglobulin variable regions and showed a restricted isoelectric focusing pattern compared with that of conventional mu chains. Intracellular mu' chains were readily detected in bone marrow cells but not in spleen or lymph node cells; mu' chains were also detected in IgM-producing tumor cells and in a hybridoma cell line that deleted its productive mu allele. These results predict irregularities in IgM structure and recall an old controversy concerning the valence of IgM molecules.

scholarly journals THE ROLE OF THYMUS AND BONE MARROW CELLS IN DELAYED HYPERSENSITIVITY

1971 ◽  
Vol 134 (5) ◽  
pp. 1144-1154 ◽  
Author(s):  
David G. Tubergen ◽  
Joseph D. Feldman
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Specific Antigen ◽  
Delayed Hypersensitivity ◽  
Cell Type ◽  
Skin Reactions ◽  
Lymph Node Cells ◽  
Thymus Cells ◽  
Marrow Cells

Adoptive transfer experiments were performed to define the immunological role of thymus and bone marrow cells in the induction of delayed hypersensitivity (DH). The results indicated the following, (a) Bone marrow from immune donors contained cells capable of being stimulated by antigen to initiate the expression of DH. (b) Bone marrow from nonimmune or tolerant donors contained cells that were needed to complete the expression of DH after the infusion of immune lymph node cells. (c) Normal bone marrow and thymus cells cooperated in the irradiated recipient to induce the most vigorous skin reactions to specific antigen; these reactions were seen only when the recipients were stimulated by antigen. Either cell type alone was ineffective. (d) In the presence of tolerant bone marrow cells, thymus cells from immune donors gave a more vigorous response than did thymus cells from normal or tolerant donors. (e) There was suggestive evidence that thymus cells were the source of trigger elements that initiated DH. (f) Antigen in the irradiated recipient was necessary to induce DH after infusion of bone marrow cells alone, or bone marrow and thymus cells together.

scholarly journals A Method of Reducing the Incidence of the Secondary Syndrome in Allogenic Marrow Transplantation

Blood ◽  
1963 ◽  
Vol 22 (1) ◽  
pp. 44-52 ◽  
Author(s):  
GEORGES MATHÉ ◽  
JEAN-LOUIS AMIEL ◽  
LÉON SCHWARZENBERG ◽  
ANNE-MARIE MERY ◽  
F. Lapeyraque
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Marrow Transplantation ◽  
Clinical Medicine ◽  
Bone Marrow Cells ◽  
Allogenic Bone ◽  
Allogenic Bone Marrow ◽  
Lymph Node Cells ◽  
Bone Marrow Grafting ◽  
Marrow Cells

Abstract Preservation of a graft of lymph node cells or semi-allogenic bone marrow cells at 37 C. in Tyrode’s solution for 2 hours, which reduces the percentage of cells not permeable to eosin by half, has a statistically significant reducing effect on the frequency of acute or chronic secondary syndromes which occur in irradiated recipients. Preservation at 18 C. for 6 hours, which in the same way increases the percentage of cells permeable to eosin, does not have the same effect. These two methods of preserving bone marrow cells do not appreciably reduce the myeloid-restoring capacity of compatible or incompatible irradiated recipients. Application of these results to bone marrow grafting in clinical medicine is discussed.

scholarly journals CYTOTOXICITY MEDIATED BY SOLUBLE ANTIGEN AND LYMPHOCYTES IN DELAYED HYPERSENSITIVITY

1968 ◽  
Vol 128 (6) ◽  
pp. 1255-1265 ◽  
Author(s):  
Nancy H. Ruddle ◽  
Byron H. Waksman
Keyword(s):  
Lymph Node ◽  
Cytotoxic Effect ◽  
Specific Antigen ◽  
Delayed Hypersensitivity ◽  
Soluble Antigen ◽  
Heterologous Proteins ◽  
Rat Embryo ◽  
Protein Carrier ◽  
Lymph Node Cells

Damage of rat embryo fibroblasts in the presence of sensitized lymph node cells reacting with specific antigen was shown to be closely correlated with delayed hypersensitivity in the animals from which the lymph node cells were taken. The phenomenon was not correlated with Arthus reactivity. In. animals sensitized with picryl conjugates of ovalbumin or human serum albumin, skin reactivity and the in vitro cytotoxic effect could be elicited only with the homologous conjugate or the protein carrier alone and not with picryl conjugates of heterologous proteins. Lewis rats developed more intense delayed sensitivity than BN rats, and Lewis lymph node cells were correspondingly more effective in producing specific damage of both syngeneic and allogeneic fibroblasts.

ABILITY OF BONE MARROW CELLS AND LYMPH NODE CELLS TO INDUCE TOLERANCE OF SKIN IN MHC-CONGENIC RATS

1993 ◽  
Vol 56 (4) ◽  
pp. 962-963
Author(s):  
LISE DESQUENNE-CLARK ◽  
HIROMITSU KIMURA ◽  
WILLYS K. SILVERS
Keyword(s):  
Bone Marrow ◽  
Lymph Node ◽  
Bone Marrow Cells ◽  
Lymph Node Cells ◽  
Marrow Cells

In vitro effects of OK-432 on irradiated mouse bone marrow cells

1994 ◽  
Vol 29 (3) ◽  
pp. 631-634 ◽  
Author(s):  
Masako Nose ◽  
Yoshiro Aoki ◽  
Yoshiko Kawase ◽  
Gen Suzuki ◽  
Makoto Akashi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Mouse Bone Marrow ◽  
In Vitro Effects ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells
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