scholarly journals QUANTITATIVE CHEMICAL STUDIES ON COMPLEMENT OR ALEXIN

1941 ◽  
Vol 73 (6) ◽  
pp. 681-694 ◽  
Author(s):  
Michael Heidelberger

1. A quantitative micro method, conforming to the criteria of analytical chemistry, is proposed for the estimation of complement, or its combining component or components, in milligrams per milliliter instead of in the customary relative and variable volume units. 2. Data are given showing the range of accuracy and reproducibility of the new method.

1942 ◽  
Vol 75 (3) ◽  
pp. 285-295 ◽  
Author(s):  
Michael Heidelberger ◽  
Manfred Mayer

1. A modified method is given for the titration of human complement so that C'1 titers are measured, as in guinea pig serum, instead of the C'2 titers yielded by the usual titration. 2. The measurement of complement combining component or components in weight units, instead of relative terms, is carried out as in guinea pig serum and leads to similar values, 0.03 to 0.05 mg. of C' N per ml. of human serum. 3. Other similarities in human and guinea pig complements are noted and discussed.


1916 ◽  
Vol 14 (2) ◽  
pp. 26-27 ◽  
Author(s):  
R. L. Kahn ◽  
J. B. Neal

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
A. Borji ◽  
Fz. Borji ◽  
A. Jourani

Analytical chemistry is a set of procedures and techniques used to identify and quantify the composition of a sample of material. It is also focused on improvements in experimental design and the creation of new measurement tools. Analytical chemistry has broad applications to forensics, medicine, science, and engineering. The objective of this study is to develop a new method of sucrose dosage using a spectrophotometry method in a pure and impure system (presence of glucose and fructose). The work performed shows the reliability of this method. A model linking sucrose solution absorbance and mass percentage of glucose and fructose has been developed using experimental design. The results obtained show that all the investigated factors (sucrose concentration, mass percentage of glucose, and mass percentage of fructose) have a positive effect on the absorbance. The effect of the interaction between glucose and fructose on the absorbance is very significant.


1943 ◽  
Vol 22 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Margaret Dann ◽  
Eleanor Marples ◽  
Samuel Z. Levine

2000 ◽  
Vol 405 (1-2) ◽  
pp. 17-21 ◽  
Author(s):  
Hirofumi Shiono ◽  
Hitoshi Nohta ◽  
Chika Utsuyama ◽  
Mitsuo Hiramatsu

1941 ◽  
Vol 73 (6) ◽  
pp. 695-709 ◽  
Author(s):  
Michael Heidelberger ◽  
Alfred J. Weil ◽  
Henry P. Treffers

1. The molecular quantities of hemolysin and complement combining component or components (C'1) involved in hemolysis have been calculated on the basis of new, quantitative, absolute methods of analysis. 2. Molecular combining ratios between antigen, antibody, and C'1 have been established. 3. The data are shown to be in accord with the theory of combination of multivalent antigen with multivalent antibody. 4. The fixation of complement by antigen-antibody combination is qualitatively and quantitatively accounted for on this basis.


1941 ◽  
Vol 74 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Michael Heidelberger ◽  
M. Rocha e Silva ◽  
Manfred Mayer

1. Quantitative data are given on the effect of variations in the time of contact and the proportions of the reactants on the quantity of complement combining component nitrogen (C'1 N) found in active guinea pig serum. 2. C'1 N was the same when determined with precipitates containing excess antibody or excess antigen. 3. Finely divided specific precipitates took up the complement combining component (C'1) from subsequently added guinea pig serum almost as well as specific precipitates formed in the presence of complement.


Biochemistry ◽  
1974 ◽  
Vol 13 (5) ◽  
pp. 1022-1027 ◽  
Author(s):  
K. R. Sommer ◽  
R. Chalkley

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