Label-Free and High-Throughput Detection of Protein Microarrays by Oblique-Incidence Reflectivity Difference Method

2010 ◽  
Vol 27 (10) ◽  
pp. 107801 ◽  
Author(s):  
Wang Xu ◽  
Lu Heng ◽  
Wen Juan ◽  
Yuan Kun ◽  
LÜ Hui-Bin ◽  
...  
Keyword(s):  
High Throughput ◽  
Difference Method ◽  
Oblique Incidence ◽  
Protein Microarrays ◽  
Label Free ◽  
High Throughput Detection

scholarly journals Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format

2007 ◽  
Vol 46 (10) ◽  
pp. 1890 ◽  
Author(s):  
Xiangdong Zhu ◽  
James P. Landry ◽  
Yung-Shin Sun ◽  
Jeff P. Gregg ◽  
Kit S. Lam ◽  
...  
Keyword(s):  
High Throughput ◽  
Oblique Incidence ◽  
Label Free ◽  
Biochemical Reactions ◽  
High Throughput Detection ◽  
Microarray Format

Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

2012 ◽  
Vol 55 (12) ◽  
pp. 2347-2350 ◽  
Author(s):  
Jun Dai ◽  
Lin Li ◽  
JingYi Wang ◽  
LiPing He ◽  
HuiBin Lu ◽  
...  
Keyword(s):  
Difference Method ◽  
Oblique Incidence ◽  
Protein Microarrays ◽  
Label Free ◽  
Label Free Detection

Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method

2010 ◽  
Vol 12 (9) ◽  
pp. 095301 ◽  
Author(s):  
Heng Lu ◽  
Juan Wen ◽  
Xu Wang ◽  
Kun Yuan ◽  
Wei Li ◽  
...  
Keyword(s):  
Difference Method ◽  
Oblique Incidence ◽  
Specific Binding ◽  
Protein Microarrays

Label-free high-throughput detection and content sensing of individual droplets in microfluidic systems

Lab on a Chip ◽  
2015 ◽  
Vol 15 (20) ◽  
pp. 4008-4019 ◽  
Author(s):  
Gurkan Yesiloz ◽  
Muhammed Said Boybay ◽  
Carolyn L. Ren
Keyword(s):  
High Throughput ◽  
Integrated Approach ◽  
Label Free ◽  
Microfluidic Systems ◽  
High Throughput Detection ◽  
Individual Droplet

We report a microwave-microfluidics integrated approach capable of detecting droplet at high-throughput and label-free sensing of individual droplet content without physical intrusion.

Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

2003 ◽  
Vol 773 ◽  
Author(s):  
James P. Landry ◽  
X. D. Zhu ◽  
X. W. Guo ◽  
J. P. Gregg
Keyword(s):  
Oblique Incidence ◽  
Protein Microarrays ◽  
Fluorescent Labeling ◽  
Fluorescent Label ◽  
Label Free ◽  
Detection Techniques ◽  
Glass Substrates ◽  
Standard Glass ◽  
Non Destructive ◽  
Microarray Detection

AbstractWe have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

Protein microarrays imaging using oblique-incidence reflectivity difference method

2007 ◽  
Author(s):  
Heng Lu ◽  
Hongyan Zhang ◽  
Wei Li ◽  
Yueliang Zhou ◽  
Huibin Lu ◽  
...  
Keyword(s):  
Difference Method ◽  
Oblique Incidence ◽  
Protein Microarrays
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