Detection of the specific binding on protein microarrays by oblique-incidence reflectivity difference method

2010 ◽  
Vol 12 (9) ◽  
pp. 095301 ◽  
Author(s):  
Heng Lu ◽  
Juan Wen ◽  
Xu Wang ◽  
Kun Yuan ◽  
Wei Li ◽  
...  
2007 ◽  
Author(s):  
Heng Lu ◽  
Hongyan Zhang ◽  
Wei Li ◽  
Yueliang Zhou ◽  
Huibin Lu ◽  
...  

2010 ◽  
Vol 53 (7) ◽  
pp. 1230-1233 ◽  
Author(s):  
Heng Lu ◽  
Juan Wen ◽  
Xu Wang ◽  
Kun Yuan ◽  
HuiBin Lu ◽  
...  

2012 ◽  
Vol 57 (22) ◽  
pp. 2898-2900 ◽  
Author(s):  
Yue Sun ◽  
LiPing He ◽  
Jun Dai ◽  
JingYi Wang ◽  
HuiBin Lü ◽  
...  

2012 ◽  
Vol 55 (12) ◽  
pp. 2347-2350 ◽  
Author(s):  
Jun Dai ◽  
Lin Li ◽  
JingYi Wang ◽  
LiPing He ◽  
HuiBin Lu ◽  
...  

2003 ◽  
Vol 773 ◽  
Author(s):  
James P. Landry ◽  
X. D. Zhu ◽  
X. W. Guo ◽  
J. P. Gregg

AbstractWe have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.


AIP Advances ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 095219 ◽  
Author(s):  
H. L. Zhan ◽  
K. Zhao ◽  
H. B. Lü ◽  
J. Zhu ◽  
K. J. Jin ◽  
...  

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