Label-free detection of biomarker alpha fetoprotein in serum by ssDNA aptamer functionalized magnetic nanoparticles

2019 ◽  
Vol 31 (9) ◽  
pp. 095104 ◽  
Author(s):  
Yu Su ◽  
Tiantian Xue ◽  
Lianpin Wu ◽  
Yunliang Hu ◽  
Jie Wang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Functionalized Magnetic Nanoparticles ◽  
Label Free Detection ◽  
Ssdna Aptamer

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

scholarly journals A Simple Displacement Aptamer Assay on Resistive Pulse Sensor for Small Molecule Detection

2020 ◽  
Author(s):  
Rhushabh Maugi ◽  
bernadette gamble ◽  
david bunka ◽  
Mark Platt
Keyword(s):  
Small Molecules ◽  
Small Molecule ◽  
Label Free ◽  
Resistive Pulse ◽  
Pulse Sensor ◽  
Label Free Detection ◽  
Ssdna Aptamer ◽  
Aptamer Assay ◽  
Small Molecule Detection ◽  
Surface Changes

A universal aptamer-based sensing strategy is proposed using DNA modified nanocarriers and Resistive Pulse Sensing for the rapid and label free detection of small molecules. The surface of a magnetic nanocarrier was first modified with a ssDNA aka linker which is designed to be partially complimentary in sequence to a ssDNA aptamer. The aptamer and linker form a stable dsDNA complex on the nanocarriers surface. Upon the addition of the target molecule, a conformational change takes place where the aptamer preferentially binds to the target over the linker; causing the aptamer to be released into solution. The RPS measures the change in velocity of the nanocarrier as its surface changes from dsDNA to ssDNA, and its velocity is used as a proxy for the concentration of the target. We illustrate the versatility of the assay by demonstrating the detection of the antibiotic Moxifloxacin, and chemotherapeutics Imatinib and Irinotecan.

Rapid and sensitive detection of E. coli O157:H7 based on antimicrobial peptide functionalized magnetic nanoparticles and urease-catalyzed signal amplification

2017 ◽  
Vol 9 (35) ◽  
pp. 5204-5210 ◽  
Author(s):  
Zhaohui Qiao ◽  
Chunyang Lei ◽  
Yingchun Fu ◽  
Yanbin Li
Keyword(s):  
Magnetic Nanoparticles ◽  
Antimicrobial Peptide ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
E Coli ◽  
Functionalized Magnetic Nanoparticles

Rapid and sensitive detection of E. coli O157:H7 based on antimicrobial peptide functionalized magnetic nanoparticles and label-free urease-catalyzed signal amplification.

Real-time detection of the interaction between alpha-fetoprotein and its ssDNA aptamer by dual polarization interferometry

2018 ◽  
Vol 42 (24) ◽  
pp. 19564-19570 ◽  
Author(s):  
Chenggang Shao ◽  
Yuxin Liu ◽  
Jinxia Qi ◽  
Yu Su ◽  
Yonghui Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Alpha Fetoprotein ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Dual Polarization Interferometry ◽  
Ssdna Aptamer ◽  
Real Time Detection

A real-time and label-free strategy to understand the interaction between biomarkers and ssDNA aptamers.

Highly sensitive label-free immunosensor for ochratoxin A based on functionalized magnetic nanoparticles and EIS/SPR detection

2011 ◽  
Vol 159 (1) ◽  
pp. 178-184 ◽  
Author(s):  
Lucian-Gabriel Zamfir ◽  
Irina Geana ◽  
Sondes Bourigua ◽  
Lucian Rotariu ◽  
Camelia Bala ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Ochratoxin A ◽  
Label Free ◽  
Functionalized Magnetic Nanoparticles ◽  
Highly Sensitive

Real-time and label-free detection of bisphenol A by an ssDNA aptamer sensor combined with dual polarization interferometry

2018 ◽  
Vol 42 (4) ◽  
pp. 2850-2856 ◽  
Author(s):  
Yu Su ◽  
Huaguo Xu ◽  
Yonghui Chen ◽  
Jinxia Qi ◽  
Xiang Zhou ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Real Time ◽  
Organic Pollutants ◽  
Label Free ◽  
Dual Polarization ◽  
Polarization Interferometry ◽  
Label Free Detection ◽  
Dual Polarization Interferometry ◽  
Ssdna Aptamer ◽  
Aptamer Sensor

DPI is an efficient and reliable platform for detection of organic pollutants and toxicants.

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