The effect of native strains of nodule bacteria on the development of symbiotic apparatus and on the productivity of new soybean cultivars

Tests of 84 cultures of root-nodule bacteria isolated from four species of clover growing in the Lismore district of New South Wales (three soil types, 18 localities) showed them to be generally effective in association with white and red clovers, but almost always parasitic on subterranean and crimson clovers. Successful seed inoculation with beneficial strains of rhizobia is, therefore, essential to the introduction of subterranean and crimson clovers in these soils. Field observations have also demonstrated the need to inoculate seed of Medicago species, cowpea, and vetch.

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Symbiotic and Agronomic Performance of Cowpea Rhizobial Inoculants from Soil of Ethiopia under Field Conditions

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2020/v32i1530374 ◽

2020 ◽

pp. 65-75

Author(s):

B. C. Nandeshwar ◽

Girmaye Kenasa ◽

Fassil Assefa

Keyword(s):

Field Condition ◽

Root Nodule ◽

Field Conditions ◽

Agronomic Performance ◽

Nodule Bacteria ◽

Chemical Fertilizers ◽

Greenhouse Condition ◽

Rhizobial Inoculation ◽

Native Strains ◽

Better Than

The effect of inoculating indigenous cowpea nodule bacteria of Ethiopia on the crop performance was not tested under field condition with the ultimate goal of selecting effective inoculants. Root nodule bacteria which showed strong symbiotic effectiveness under greenhouse condition were selected for field experiment. Under field conditions, inoculation of cowpea with ECR-0+ECE-21 and ECR-101+ECE-21 resulted in a maximum grain yield (GY, 2713 kg ha-1) and above ground biomass (BW, 506 g m-2), respectively. The co-inoculation of cowpea with ECR-0 and ECE-21 and ECR-24 and ECE-21 significantly improved (p<0.05) the N-content and GY as compared to their single rhizobial inoculation. The native strains performed better than the exotic strain (Biofix). Performance of the strains was similar at the two test locations except ECR-14 and ECR-24 that resulted 13% greater of BW at Uke than at Bako. Symbio-agronomic performance of native rhizobia under field condition showed direct correlation (r>0.5; p<0.01) to each other except nodule number. In general, both single and co-inoculation of the native rhizobacterial inoculants improved cowpea performance. This is a promising biofertilizer to enhance cowpea production in Ethiopia, where the farmers cannot afford to buy chemical fertilizers.

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The root-nodule bacteria as factors in clover establishment in the red basaltic soils of the Lismore district, NSW. II. Survival and success of inocula in laboratory trials.

Australian Journal of Agricultural Research ◽

10.1071/ar9540061 ◽

1954 ◽

Vol 5 (1) ◽

pp. 61 ◽

Cited By ~ 29

Author(s):

JM Vincent ◽

LM Waters

Keyword(s):

Root Nodule ◽

Nodule Formation ◽

Nodule Bacteria ◽

Root Nodule Bacteria ◽

Reasonable Degree ◽

Seed Inoculation ◽

Serological Identification ◽

Small Rise ◽

Basaltic Soils ◽

Native Strains

Plant growth, nature of nodulation, and serological identification of cultures isolated from nodules have been used to determine the success achieved with inocula of clover rhizobia. The Lismore krasnozems offer difficulties in the way of successful seed inoculation. Milk and phosphate supplements with the inoculum do not obviate the difficulty, but heavier rates of inoculation give a reasonable degree of nodulation even with untreated soils. The unfavourable nature of these soils is reflected in their relatively low rhizobial population and the failure of added cells to persist or multiply at a rate comparable with other soils and conditions. Raising pH to c. 7 by the addition of Ca(OH)2 or CaCO3 markedly improves the soil as a medium for survival and multiplication of clover rhizobia. Even a small rise in pH gives appreciable improvement. Strains of root-nodule bacteria differ markedly in their ability to compete with each other for growth and nodule formation on a host. The relative performance of strains can be affected by such factors as the nature of the soil and the presence and nature of the host. Competition for nodulation need not be related in any simple way to competition for growth. Competition by :"native" strains can be an important factor contributing to success or failure with added inoculum.

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Affect of Rhizotorphin on chlorophyll content and productivity of introduced soybean cultivars

RUDN Journal of Agronomy and Animal Industries ◽

10.22363/2312-797x-2020-15-1-7-18 ◽

2020 ◽

Vol 15 (1) ◽

pp. 7-18

Author(s):

Yulia Vladislavovna Tsvetkova ◽

Marina Ustimovna Lyashko ◽

Inna Ivanovna Strazhnikova

Keyword(s):

Nitrogen Content ◽

Root Nodule ◽

Root Nodules ◽

N Fixation ◽

Nodule Bacteria ◽

Soybean Cultivars ◽

Biological N Fixation ◽

Seed Inoculation ◽

Soybean Leaves ◽

Seed Yields

Biotic nitrogen fi is a phenomenon mainly depended on mutualistic interrelation between host plant and root nodule bacteria. This interrelation affects photosynthesis and productivity of biological N-fixation and requires studies of biological particularities of grown legume cultivars and effectiveness of Bradyrhizobium strains used for seed inoculation. A field experiment was conducted on the territory of Nesvizhskaya Crop Testing Station (Minsk region, Republic of Belarus). Based on chlorophyll and nitrogen content in soybean leaves, number of nodules on roots of cultivars grown, and on quantity of nitrogen accumulated in plants, four soybean cultivars were assessed. The cultivar Pripyat (control) has poorly responded on inoculation, whereas cultivars Slavyankа an Sobrini responded well by increase of chlorophyll and nitrogen content in leaves and by larger number of root nodules. As a result, they produced unusually high seed yields. Therefore, these soybean cultivars may be recommended for wide use in regions of Belarus.

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The effects of high temperatures on certain root-nodule bacteria

Australian Journal of Agricultural Research ◽

10.1071/ar9670299 ◽

1967 ◽

Vol 18 (2) ◽

pp. 299 ◽

Cited By ~ 21

Author(s):

J Wilkins

Keyword(s):

New England ◽

High Temperatures ◽

Root Nodule ◽

New South ◽

New South Wales ◽

Nodule Bacteria ◽

Natural Conditions ◽

Root Nodule Bacteria ◽

South Wales ◽

Native Strains

Rhizobia present in soils from western New South Wales and from the New England Tableland were subjected to a series of high temperature survival tests. Rhizobia present in air-dry soils survived temperatures higher than would be experienced under natural conditions. In moist soils the tolerance of medic rhizobia to high temperatures was much lower. Strains of Acacia, Lotus and Psoralea rhizobia from western New South Wales survived higher temperatures than strains from the New England Tableland, but the tolerance to high temperatures of medic rhizobia did not vary with source. This suggests an ecological adaptation of the three native strains not found in the introduced Medicago rhizobium.

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